2007 Fiscal Year Final Research Report Summary
Reasrach on Biosynthetic Pathway of Betalains
| Project/Area Number |
18370017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
YOSHIHIRO Ozeki Tokyo University of Agriculture and Technology, The Institute of Symbiotic Science and Technology, Professor (50185592)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Hiroaki Chiba University, Graduate School of Horticulture, Associate Professor (70302536)
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| Project Period (FY) |
2006 – 2007
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| Keywords | betalain / biosynthesis / cyclo-DOPA / four o'clocks / globe amaranth / glucosyltransferase / phenylpropanoid |
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| Research Abstract |
cDNAs encoding for the homologous gene of DOPA 4,5 dioxygenase (DOD), which was a key enzyme involved in betalain biosynthesis, were isolated from four o'clocks, Arabidopsis, torenia and carnation. The cDNA was introduced into a expression vector and transformed into Escherichia coli In the crude extract prepared from the E. coli, DOD activity was detected to form betalamic acid, indicating that the enzymatic properties of DOD could be elucidated using this extract in vitro. Because betalains were modified by sugar moieties at the intermediate step of cyclo-DOPA of the synthetic pathway, cDNAs for cyclo-DOPA glucosyltransferase (cDOPA5GT) were isolated from four o'clocks, portulaca and globe amaranth. The amino acid sequences of cDOPA5GT cDNAs derived from these plant species consisted of a separated clade from others, suggesting that cDOPA5GT might have arisen early during the evolutionary divergence and established of the Caryophyllales except for Dianthus. The specific activities of other GTs encoded in cDNAs derived from these species isolated simultaneously were analyzed by heterologous expression in E. coli. The GT encoded in a cDNA derived from globe amaranth showed broad specific activity to phenylpropanoids including p-coumaric acid, caffeic acid, sinapic acid and ferulic acid as sugar acceptors. Using this cDNA together with the cDNA of Arabidopsis sucrose synthase, the efficient synthetic method for phenylpropanoid-glucosides was established. These phenylpropanoid-glucosides purified by high performance liquid chromatography could be used as good acyl-donors for anthocyanin acyltransfarase assays.
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[Journal Article] Enzymatic preparation of 1-O-hydroxycinnamoyl-β-D-gluooses and their application to the study of 1-O-hydroxycinnamoyl-β-D-gluucose-dependent acyltransferase inanthocyanin-producing cultured cells of Daucus carota and Glehnia littoralis.2008
Author(s)
Matsuba, Y., Okuda, Y., Abe, Y., Kitamura, Y., Terasaka, K., Mizukami, H., Kamakura, H., Kawahara, N., Goda, Y., Sasaki, N. and Ozeki, Y.
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Journal Title
Plant Biotechnol. 25(印刷中)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Enzymatic preparation of 1-O-hydroxycinnamoy1-β-D- glucoses and their application to the study of 1-O-hydroxycinnamoyl-β-D-glucose-dependent acyltransferase in anthocyanin-producing cultured cells of Daucus carota and Glehnia littoralis.2008
Author(s)
Y., Matsuba, Y., Okuda, Y., Abe, Y., Kitamura, K., Terasaka, H., Mizukami, H., Kamakura, N., Kawahara, Y., Goda, N., Sasaki, Y., Ozeki
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Journal Title
Plant Biotechnol. 25(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulation of anthocyanin biosynthesis involved in the formation of marginal picoteepetals in Petunia.2006
Author(s)
Saito, R., Fukuta, N., Ohmiya, A., Itoh, Y., Ozeki, Y., Kuchitsu, K. and Nakayama, M.
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Journal Title
Plant Science 170
Pages: 828-834
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Identification and characterization of genes induced for anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots using suppression subtractive hybridization, cDNA microarrays, and RNAi techniques.2006
Author(s)
Nagira, Y., Shimamura, K., Hirai, S., Shimanuki, M., Kodama, H. and Ozeki, Y.
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Journal Title
J. Plant Res. 119
Pages: 217-230
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Regulation of anthocyanin biosynthesis involved in the formation of marginal picotee petals in Petunia.2006
Author(s)
R., Saito, N., Fukuta A., Ohmiya, Y., Itoh, Y., Ozeki, K., Kuchitsu, M., Nakayama
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Journal Title
Plant Science 170
Pages: 828-834
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of genes induced for anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots using suppression subtractive hybridization, cDNA microarrays, and RNAi techniques.2006
Author(s)
Y., Nagira, K., Shimamura, S., Hirai, M., Shimanuki, H., Kodama, Y., Ozeki
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Journal Title
J. Plant Res. 119
Pages: 217-230
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Enzymatic preparation of 1-O-hydroxycinnamoy1-β-D-glucoses and their application to the study of 1-O-hydroxycinnamoy1-β-D-glucose-dependent acyltransferase in anthocyanin-producing cultured cells of Daucus carota and Glehnia littoralis.2008
Author(s)
Y., Matsuba, Y., Okuda, Y., Abe, Y., Kitamura, K., Terasaka, H., Mizukami, H., Kamakura, N., Kawahara, Y., Goda, N., Sasaki, Y., Ozeki
Organizer
The 49th Annual Meeting of Japanese Society of Plant Physiologists.
Place of Presentation
Sapporo
Year and Date
2008-03-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A transcription regulatory factor on expression of DcMYBJ gene induced by stresses in carrot suspension cultured cells.2007
Author(s)
S., Sato, K., Maeda, S., Kimura, W., Noma, N., Sasaki, Y., Ozeki
Organizer
The 25th Annual Meeting of Japanese Society of Plant Cell and Molecular Biology
Place of Presentation
Chiba
Year and Date
2007-08-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Isolation of DOPA 4,5-dioxygenase genes of higher plants.2007
Author(s)
N., Sasaki, Y., Nagira, K., Yabuki, T., Koda, T., Adachi, Y., Ozeki
Organizer
The 48th Annual Meeting of Japanese Society of Plant Physiologists
Place of Presentation
Matsuyama
Year and Date
2007-03-28
Description
「研究成果報告書概要(欧文)」より
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