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2007 Fiscal Year Final Research Report Summary

Studies on novel functions of plant peroxisomes

Research Project

Project/Area Number 18370021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionNational Institute for Basic Biology

Principal Investigator

NISHIMURA Mikio  National Institute for Basic Biology, Department of Cell Biology, Professor (80093061)

Project Period (FY) 2006 – 2007
Keywordsperoxisome / peroxisome targeting signal / PEX gene / polyamine metabolism / proteome / protein transport / morphogenesis of organelles
Research Abstract

1) Almost all of the peroxisomal matrix proteins are known to contain one of two targeting signals(PTS1 and PTS2) within the molecules. We identified 256 gene candidates of PTS1- and PTS2- containing proteins and another 30 genes of non-PTS-containing proteins from Arabidopsis genome. Custom-made DNA microarray covering all these genes was used to investigate expression profiles of the peroxisomal genes in various organs.
2) Bioinfomatic analysis of Arabidopsis genome predicted the presence of 15kinds of genes, called PEX genes, for peroxisomal biogenesis factors. We comprehensively investigated whether or not these predicted PEX genes function in peroxisome biogenesis by generating knock-down mutants that suppress PEX gene expression by RNA-interference. Phenotypes of these mutants allowed us to identify the functional PEX genes, which can be classified into two groups, i.e. PEX genes regulating for peroxisomal morphology and PEX genes regulating for peroxisomal protein import.
3) To clarify the function of root peroxisomes, we characterized a peroxisomal polyamine oxidase, AtPAO4 gene that is expressed in roots of Arabidopsis thaliana. This indicates that root peroxisomes are involved in polyamine metabolism.
4) We also made a two-dimensional protein map of glyoxysomes isolated from soybean. Peptide MS fingerprinting analyses allowed us to identify novel proteins existing in either glyoxysomes. Some of these proteins contain no obvious PTS1 and PTS2. Combination of the transcriptomic and proteomic analyses is providing us with a new insight into plant peroxisomal functions.

  • Research Products

    (52 results)

All 2008 2007 2006 Other

All Journal Article (13 results) (of which Peer Reviewed: 6 results) Presentation (38 results) Book (1 results)

  • [Journal Article] The plant organelles database(PODB):A collection of visualized plant organelles and protocols for plant organelle research2008

    • Author(s)
      Mano Shoji
    • Journal Title

      Nucleic Acid Res. 36

      Pages: D929-937

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons2008

    • Author(s)
      Arai Yuko
    • Journal Title

      Plant Cell Physiol 49

      Pages: 526-539

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but distinct from typical PTS1 import2008

    • Author(s)
      Oshima Yoshimi
    • Journal Title

      Plant Cell Physiol 49

      Pages: 671-677

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but distinct from typical PTSlimport.2008

    • Author(s)
      Oshima Yoshimi
    • Journal Title

      Plant Cell Physiol 49

      Pages: 671-677

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons.2008

    • Author(s)
      Arai Yuko
    • Journal Title

      Plant Cell Physiol 49

      Pages: 526-539

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The plant organelles database(PODB) : A collection of visualized plant organelles and protocols for plant organelle research.2008

    • Author(s)
      Mano Shoji
    • Journal Title

      Nucleic Acid Res. 36

      Pages: D929-937

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional identification of Arabidopsis peroxisome biogenesis factors using RNAi2007

    • Author(s)
      Nito Kazumasa
    • Journal Title

      Plant Cell Physiol. 48

      Pages: 763-774

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] HSP90 regulates the heat shock response in Arabidopsis thaliana that is responsible for heat adaptation2007

    • Author(s)
      Yamada Kenji
    • Journal Title

      J.Biol.Chem. 282

      Pages: 37794-37804

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] HSP9O regulates the heat shock response in Arabidopsis thaliana that is responsible for heat adaptation.2007

    • Author(s)
      Yamada Kenji
    • Journal Title

      J. Biol. Chem. 282

      Pages: 37794-37804

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional identification of Arabidopsis peroxisome biogenesis factors using RNAi.2007

    • Author(s)
      Nito Kazumasa
    • Journal Title

      Plant Cell Physiol. 48

      Pages: 763-774

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Arabidopsis pex12 and pex13 mutants are defective in both PTS1 and PTS-2dependent transport to peroxisomes2006

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant J. 47

      Pages: 1187-1194

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The Arabidopsis pexl2 and pexl3 mutants are defective in both PTS1 and PTS-2dependent transport to peroxisomes.2006

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant J. 47

      Pages: 1187-1194

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Plant Proteomics : Technologies, Strategies and Applications

    • Author(s)
      Arai Yuko
    • Journal Title

      John Wiley&Sons (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation2007

    • Author(s)
      Yamada Kenji
    • Organizer
      18th International Conference on Arabidopsis Research
    • Place of Presentation
      Beijing,China
    • Year and Date
      20070719-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation.2007

    • Author(s)
      Yamada Kenji
    • Organizer
      18th interbational Conference on Arabidopsis Research
    • Place of Presentation
      Beijing, China
    • Year and Date
      20070719-24
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Establishment of peroxisomal functions by peroxisomal biogenesisfactors.2007

    • Author(s)
      Hayashi Makoto
    • Organizer
      Spotlight on Japanese Plant Science
    • Place of Presentation
      Beijing, China
    • Year and Date
      20070719-24
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of Arabidopsis thaliana that was suppressed PEX10 gene expression2007

    • Author(s)
      Kamigaki Akane
    • Organizer
      Plant Biology and Botany 2007
    • Place of Presentation
      Chicago,USA
    • Year and Date
      20070709-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of Arabidopsis thaliana that was suppressed PEX10 gene expression.2007

    • Author(s)
      Kamigaki Akane
    • Organizer
      Plant Biology and Botany 2007
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20070709-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Identification and characterization of factors responsible for peroxisome biogenesis using arabidopsis apm mutants2007

    • Author(s)
      Mano Shoji
    • Organizer
      Global Opportunities Fund Collaboration Development Awards
    • Place of Presentation
      Leeds,England
    • Year and Date
      2007-11-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Identification and characterization of factors responsible for peroxisome biogenesis using arabidopsis apm mutants.2007

    • Author(s)
      Mano Shoji
    • Organizer
      Global Opportunities Fund Collaboration
    • Place of Presentation
      Leeds, England
    • Year and Date
      2007-11-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Establishment of peroxisomal functions by peroxisomal biogenesis factors2007

    • Author(s)
      Hayashi Makoto
    • Organizer
      Spotlight on Japanese Plant Science
    • Place of Presentation
      Leeds,UK
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Transcriptomic-and proteomicanalyses revealed unidentified functions of plant peroxisomes.2006

    • Author(s)
      Arai Yuko
    • Organizer
      International Symposium of Plant Molecular Biology (ISPMB)
    • Place of Presentation
      Adelaide, Australia
    • Year and Date
      20060820-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Functional Chracterization of Arabidopsis Peroxisome Biogenesis Factors.2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      International Symposium of Plant Molecular Biology (ISPMB)
    • Place of Presentation
      Adelaide, Australia
    • Year and Date
      20060820-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] HSP90 regulates stress response to adapt a high temperature in Arabidopsis2006

    • Author(s)
      Yamada Kenji
    • Organizer
      Plant Biology 2006 (ASPP 2006 Annual Meeting)
    • Place of Presentation
      Boston,USA
    • Year and Date
      20060804-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Plant organelles database2006

    • Author(s)
      Nishikawa Shuh-ichi
    • Organizer
      Plant Biology 2006 (ASPP 2006 Annual Meeting)
    • Place of Presentation
      Boston,USA
    • Year and Date
      20060804-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Plant organelles database2006

    • Author(s)
      Shuh-ichi Nishikawa
    • Organizer
      Plant Biology 2006
    • Place of Presentation
      Boston, USA
    • Year and Date
      20060804-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] HSP90 regulates stress response to adapt a high temperature in Arabidopsis2006

    • Author(s)
      Yamada Kenji
    • Organizer
      Plant Biology 2006
    • Place of Presentation
      Boston, USA
    • Year and Date
      20060804-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Establishment of peroxisomal functions by peroxisomal biogenesis factors2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      VIII Spanish Congress on Plant Molecular Biology
    • Place of Presentation
      Pamplona,Spain
    • Year and Date
      20060628-0701
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Establishment of peroxisomal functions by peroxisomal biogenesisfactors.2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      VIII Spanish Congress on Plant Molecular Biology
    • Place of Presentation
      Pamplona, Spain
    • Year and Date
      20060628-0701
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Functional Chracterization of Arabidopsis Peroxisome Biogenesis Factors2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      International Symposium of Plant Molecular Biology(ISPMB)
    • Place of Presentation
      Adelaide,Australia
    • Year and Date
      20060620-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Transcriptomic-and proteomicanalyses revealed unidentified functions of plant peroxisomes2006

    • Author(s)
      Arai Yuko
    • Organizer
      International Symposium of Plant Molecular Biology (ISPMB)
    • Place of Presentation
      Adelaide,Australia
    • Year and Date
      20060620-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of plant peroxisomesusing post-genomic techniques2006

    • Author(s)
      Arai Yuko
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto,Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Role of Arabidopsis HSP90 in the response to environmental stimuli2006

    • Author(s)
      Yamada Kenji
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto,Japan
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Investigation for function in root peroxisomes classified by transcriptomic-analyses of peroxisomal proteins2006

    • Author(s)
      Kamada-Nobusada Tomoe
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Knockdown mutants generated by RNA interference allowed functinonl classification of Arabidopsis PEX genes2006

    • Author(s)
      Arai Yuko
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Comprehensive identification of unknown proteins/genes involved in plant peroxiomal function2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation in Arabidopsis2006

    • Author(s)
      Yamada Kenji
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] HSP90 regulates heat shock response that is responsible for heat adaptation in Arabidopsis.2006

    • Author(s)
      Yamada Kenji
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Comprehensive identification of unknown proteins/genes involved in plant peroxiomal function.2006

    • Author(s)
      Hayashi Makoto
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Knockdown mutants generated by RNA interference allowed functinonl classification of Arabidopsis PEX genes.2006

    • Author(s)
      Arai Yuko
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Investigation for function in root peroxisomes classified by transcriptomic-analyses of peroxisomal proteins.2006

    • Author(s)
      Kamada-Nobusada Tomoe
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Biogenesis and differentiation of plant peroxisomes.2006

    • Author(s)
      Nishimura Mikio
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Construction of plant organelles database : Database of plant organelles visualized with fluorescent probes and database of protocols for functional analysis.2006

    • Author(s)
      Mano Shoji
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak, Japan
    • Year and Date
      20060614-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] CHUP1 Fun ction on the Chloroplast and Anchor Chloroplast at the Periclinal Plasma Membrane Localization2006

    • Author(s)
      Oikawa Kazusato
    • Organizer
      International Symposium of Plant Molecular Biology (ISPMB)
    • Place of Presentation
      Adelaide,Australia
    • Year and Date
      2006-08-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Construction of plant organelles database" 20th2006

    • Author(s)
      Mano Shoji
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto,Japan
    • Year and Date
      2006-06-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Construction of plant organelles database.2006

    • Author(s)
      Mano Shoji
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Role of Arabidopsis HSP90 in the response to environmental stimuli.2006

    • Author(s)
      Yamada Kenji
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Functional analysis of plant peroxisomesusing post-genomic techniques.2006

    • Author(s)
      Arai Yuko
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Biogenesis and differentiation of plant peroxisomes2006

    • Author(s)
      Nishimura Mikio
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Construction of plant organelles database:Database of plant organelles visualized with fluorescent probes and database of protocols for functional analysis2006

    • Author(s)
      Mano Shoji
    • Organizer
      The 53rd NIBB conference-Dynamic organelles in plants
    • Place of Presentation
      Okazak,Japan
    • Year and Date
      2006-06-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] CHUP1 Fun ction on the Chloroplast and Anchor Chloroplast at thePericlinal Plasma Membrane Localization

    • Author(s)
      Oikawa Kazusato
    • Organizer
      International Symposium of Plant Molecular Biology (ISPMB)
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Plant Proteomics:Technologies, Strategies and Applications

    • Author(s)
      Arai Yuko
    • Publisher
      John Wiley&Sons
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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