Function of neuregulin, a new paracrine factor, in spermatogenesis

2008 Fiscal Year Final Research Report

• Project/Area Number: 18370025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Kumamoto University
• Principal Investigator: ABE Shin-ichi Kumamoto University, 大学院・自然科学研究科, 教授 (90109637)
• Co-Investigator(Kenkyū-buntansha): ETO Ko 熊本大学, 大学院・自然科学研究科, 助教 (40359964)
• Project Period (FY): 2006 – 2008
• Keywords: イモリ / マウス / FSH / neuregulin / retinoic acid / プロラクチン / アポトーシス / RNA結合タンパク質

Research Abstract

イモリ精巣と生後マウス精巣の培養系を用いてneuregulin(NRG)1やretinoic acid(RA)の減数分裂開始に対する作用を解析した。NRG1やRAはイモリやマウス精原細胞の増殖を直接促進する一方、セルトリ細胞を介して間接的に減数分裂を開始させること、NRG1はRAの下流で働くことが分かった。またプロラクチンによるイモリ精原細胞のアポトーシス誘起機構は、RNA結合タンパク質を介することによる。

Research Products

• [Journal Article] The roles of pericystic cells and Sertoli cells in spermatogonial proliferation stimulated by some growth factors in organ culture of newt(Cynops pyrrhogaster)testis (2008)
  • Author(s): Li, Y., Oral, O., Abe, K., Eto, K., Abe, S.-I
  • Journal Title: Gen. Comp. Endocrinol 159 Pages: 80-87
  • Peer Reviewed
• [Journal Article] Promotion of spermatogonial proliferation by Neuregulin 1 in newt(Cynops pyrrhogaster)testis (2008)
  • Author(s): Oral, O., Uchida, I., Eto, K., Nakayama, Y., Nishimura, O., Hirao, Y., Ueda, J., Tarui, H., Agata, K., Abe, S.-I
  • Journal Title: Mech. Dev. 2008 125 Pages: 906-917
  • Peer Reviewed
• [Journal Article] Purification and cDNA cloning of a novel protease inhibitor secreted into culture supernatant by MDCK cells (2008)
  • Author(s): Nishiyama, K., Sugawara, K., Nouchi, T., Kawano, N., Soejima, K., Abe, S.-I., Mizokami, H
  • Journal Title: Biologicals 36 Pages: 122-133
  • Peer Reviewed
• [Journal Article] Epidermal growth factor mediates spermatogonial proliferation in newt testis (2008)
  • Author(s): Abe, K., Eto, K., Abe, S.-I
  • Journal Title: Reproductive Biology and Endocrinology 6 Pages: 7
  • Peer Reviewed
• [Journal Article] Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka(Oryzias latipes) (2008)
  • Author(s): Shiraishi, E., Yoshinaga, N., Miura, T., Yokoi, H., Wakamatsu, Y., Abe, S.-I., Kitano, T
  • Journal Title: Endocrinology 149 Pages: 1813-1819
  • Peer Reviewed
• [Journal Article] Size-selective junctional barrier and Ca2+-independent cell adhesion in the testis of Cynops pyrrhogaster : expression and function of occludin (2008)
  • Author(s): Jin, Y., Uchida, I., Eto, K., Kitano, T., Abe, S.-I
  • Journal Title: Mol. Reprod. Dev 75 Pages: 202-216
  • Peer Reviewed
• [Journal Article] Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and Mullerian inhibiting substance mRNA expression in Japanese flounder(Paralichthys olivaceus) (2007)
  • Author(s): Kitano, T., Yoshinaga, N., Shiraishi, E., Koyanagi, T., Abe, S.-I
  • Journal Title: Mol. Reprod. Dev 74 Pages: 1171-1177
  • Peer Reviewed
• [Journal Article] Functional demonstration of the ability of a primary spermatogonium as a stem cell by tracing a single cell destiny in Xenopus laevis. Develop (2006)
  • Author(s): Kawasaki T., Imura, F., Nakada, A., Kubota, H., Sakamaki, K., Abe, S.-I., Takamune, K
  • Journal Title: Growth, Differ 48 Pages: 525-535
  • Peer Reviewed
• [Journal Article] The immunoglobulin-like domain is involved in interaction of Neuregulin1 with ErbB (2006)
  • Author(s): Eto, K., Eda, K., Kanemoto, S., Abe, S.-I
  • Journal Title: Biochem. Biophys. Res. Comm 350 Pages: 263-271
  • Peer Reviewed
• [Journal Article] HSP90b is involved in signaling prolactin-induced apoptosis in newt testis (2006)
  • Author(s): Saribek, B., Jin, Y., Saigo, M., Eto, K., Abe, S.-I
  • Journal Title: Biochem. Biophys. Res. Comm 349 Pages: 1190-1197
  • Peer Reviewed
• [Journal Article] Assessment of estrogenic chemicals using an estrogen receptor α(ERα)- and ER β-mediated reporter gene assay in fish (2006)
  • Author(s): Kitano, T., Koyanagi, T., Adachi, R., Sakimura, N., Takamune, K. and Abe, S.-I
  • Journal Title: Marine Biology 149 Pages: 49-55
  • Peer Reviewed
• [Presentation] マウス精原細胞の増殖におけるErbB4受容体を介した細胞間、及び細胞内シグナル伝達機構の解析 (2008)
  • Author(s): 本明あすか
  • Organizer: 日本分子生物学会第31回大会
  • Place of Presentation: 神戸市
  • Year and Date: 20081200
• [Presentation] イモリ精原細胞におけるアポトーシスのRNA結合タンパク質を介した制御機構 (2008)
  • Author(s): 後藤翔太
  • Organizer: 日本比較内分泌学会第33回大会
  • Place of Presentation: 広島市
  • Year and Date: 20081200
• [Presentation] アフリカツメガエル変態過程におけるRNA結合タンパク質XCIRPの発現とアポトーシスの関係について (2008)
  • Author(s): 岩間智之
  • Organizer: 日本比較内分泌学会第33回大会
  • Place of Presentation: 広島市
  • Year and Date: 20081200
• [Presentation] イモリSertoli細胞における減数分裂開始/アポトーシス誘導関連タンパク質の2D-DIGE(2 Dimensional Fluorescence Difference Gel Electrophoresis)法による網羅的解析 (2008)
  • Author(s): 白濱陽一郎
  • Organizer: 日本比較内分泌学会第33回大会
  • Place of Presentation: 広島市
  • Year and Date: 20081200
• [Presentation] ErbB4の細胞内領域p8はカルパインで分解される (2008)
  • Author(s): 園田祥之
  • Organizer: 日本動物学会79回大会
  • Place of Presentation: 福岡市
  • Year and Date: 20080900
• [Presentation] イモリ精巣器官培養において精原細胞の増殖や分化を誘導する増殖因子の標的細胞の解析 (2008)
  • Author(s): 李ユブン
  • Organizer: 日本動物学会79回大会
  • Place of Presentation: 福岡市
  • Year and Date: 20080900
• [Presentation] マウス精巣におけるNociceptinの発現制御と機能の解析 (2008)
  • Author(s): 衛藤豪克
  • Organizer: 日本動物学会79回大会
  • Place of Presentation: 福岡市
  • Year and Date: 20080900
• [Presentation] Effect of NRGs and RA on spermatogonial proliferation and meiosis initiation in culture of neonatal mouse testis (2008)
  • Author(s): Jidong Zhang
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島市
  • Year and Date: 20080500
• [Presentation] Epidermal growth factor mediates spermatogonial proliferation in newt testis (2008)
  • Author(s): Keisuke Abe
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島市
  • Year and Date: 20080500
• [Presentation] Reduced expression of a conserved RNA-binding protein by prolactin leads to apoptosis in newt spermatogonia (2008)
  • Author(s): Ko Eto
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島市
  • Year and Date: 20080500
• [Presentation] Binary Switch Mechanism toward Meiosis or Apoptosis in Late Spermatogonia in the Testes in Japanese Newt (2007)
  • Author(s): Abe, S.-I
  • Organizer: Cynops pyrrhogaster. 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY
  • Place of Presentation: Siliguri, India
  • Year and Date: 20071200
• [Presentation] Transcriptional repression of a conserved RNA binding protein by prolactin leads to apoptosis in newt spermatogonia (2007)
  • Author(s): Eto, K
  • Organizer: 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY
  • Place of Presentation: Siliguri, India
  • Year and Date: 20071200
• [Presentation] Signaling Mechanism of Neuregulin1-dependent proliferation in Newt(Cynops pyrrhogaster)Spermatogonia (2007)
  • Author(s): Oral, O
  • Organizer: 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY
  • Place of Presentation: Siliguri, India
  • Year and Date: 20071200
• [Presentation] Neuregulins Promote Mouse Spermatogonial Proliferation and Expression of Meiosis-Specific Genes in Vitro (2007)
  • Author(s): Zhang, J
  • Organizer: 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY
  • Place of Presentation: Siliguri, India
  • Year and Date: 20071200
• [Presentation] Xtr protein, One of the key Molecules for Progression of Meiotic Cycle in Xenopus laevis (2007)
  • Author(s): Mostafa, G
  • Organizer: 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY
  • Place of Presentation: Siliguri, India
  • Year and Date: 20071200
• [Presentation] イモリ精原細胞のアポトーシスにおけるHSP90との結合タンパク質の単離、同定 (2007)
  • Author(s): 深浦一幸
  • Organizer: 日本比較内分泌学会第32回大会
  • Place of Presentation: 日光
  • Year and Date: 20071000
• [Presentation] イモリ精巣器官培養におけるEGFの精原細胞増殖促進効果 (2007)
  • Author(s): 安部恵祐
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
  • Year and Date: 20070900
• [Presentation] イモリ精原細胞における減数分裂開始かアポトーシスかの運命決定 (2007)
  • Author(s): 安部眞一
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前
  • Year and Date: 20070900
• [Presentation] Protective role of a conserved RNA binding protein in spermatogonial apoptosis induced by prolactin during newt spermatogenesis (2007)
  • Author(s): Ko Eto
  • Organizer: 日本発生生物学会第40回大会
  • Place of Presentation: 福岡
  • Year and Date: 20070500
• [Presentation] Neuregulinsはマウス器官培養において減数分裂の開始を促進する (2007)
  • Author(s): 張継東
  • Organizer: 日本発生生物学会第40回大会
  • Place of Presentation: 福岡
  • Year and Date: 20070500
• [Presentation] Protective role of a conserved RNA binding protein in spermatogonial apoptosis induced by prolactin during newt spermatogenesis (2007)
  • Author(s): Toyooka, I
  • Organizer: International Symposium on Amphibian and Reptile Endocrinology and Neurobiology
  • Place of Presentation: Berkeley, USA
  • Year and Date: 20070300
• [Presentation] The expression of prolactin receptor isoforms and the role of Sertoli cells in the apoptosis of spermatogonia in the testis of Japanese newt, Cynops pyrrhogaster (2007)
  • Author(s): Nishi, H
  • Organizer: International Symposium on Amphibian and Reptile Endocrinology and Neurobiology
  • Place of Presentation: Berkeley, USA
  • Year and Date: 20070300
• [Presentation] The function of Stem Cell Factor and Neuregulin in the proliferation and differentiation of newt spermatogonia by using culture system (2007)
  • Author(s): Uchida, I
  • Organizer: International Symposium on Amphibian and Reptile Endocrinology and Neurobiology
  • Place of Presentation: Berkeley, USA
  • Year and Date: 20070300
• [Presentation] Binary switch mechanism toward meiosis or apoptosis in late spermatogonia in the testes in Japanese newt, Cynops pyrrhogaster (2007)
  • Author(s): Abe, S.-I
  • Organizer: International Symposium on Amphibian and Reptile Endocrinology and Neurobiology
  • Place of Presentation: Berkeley, USA
  • Year and Date: 20070300
• [Presentation] HSP90 is involved in signaling prolactin-induced apoptosis in newt testis (2006)
  • Author(s): 江頭恒
  • Organizer: 日本比較内分泌学会第31回大会
  • Year and Date: 20061100
• [Presentation] イモリセルトリ細胞の培養 (2006)
  • Author(s): 内田一郎
  • Organizer: 日本動物学会第77回大会
  • Place of Presentation: 松江
  • Year and Date: 20060900
• [Presentation] Neuregulins promote spermatogonial proliferation and expression of meiosis-specific gene Spo11 during mouse spermatogenesis (2006)
  • Author(s): Ko Eto
  • Organizer: 23rd CONFERENCE OF EUROPEAN COMPARATIVE ENDOCRINOLOGISTS
  • Place of Presentation: Manchester, UK
  • Year and Date: 20060800
• [Presentation] Neuregulins promote spermatogonial proliferation and expression of meiosis-specific gene Spo11 during mouse spermatogenesis (2006)
  • Author(s): Jidong Zhang
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20060600
• [Presentation] Role of the Hsp90b in Prolactin-Induced Germ Cell Apoptosis during Spermatogenesis of Japanese Red-Bellied Newt(Cynops pyrrhogaster) (2006)
  • Author(s): Buget SARIBEK
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20060600
• [Presentation] イモリ精原細胞の減数分裂開始におけるイモリstem cell factorとneuregulinの作用 (2006)
  • Author(s): 内田一郎
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060600
• [Presentation] アフリカツメガエルの生殖細胞と初期胚で特異的に発現するXtrタンパク質の機能阻害による減数分裂抑制 (2006)
  • Author(s): 大神浩幹
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060600
• [Presentation] 雄性生殖細胞の純化の試み (2006)
  • Author(s): 河崎敏広
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060600
• [Presentation] メダカ性分化過程におけるミュラー管抑制物質受容体の発現・機能解析 (2006)
  • Author(s): 白石絵吏
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060600
• [Presentation] Neuregulinはマウス精子形成過程において精原細胞の増殖と減数分裂の開始に特異的な遺伝子の発現を促進する (2006)
  • Author(s): 張継東
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060600
• [Book] 第6章脊椎動物の精子分化-イモリ精巣をモデルにしてー、21世紀の動物科学。第4巻「性と生殖」(安部眞一、星元紀編) (2007)
  • Author(s): 安部眞一
  • Total Pages: 169-201(243)
  • Publisher: 培風館
• [Book] 第3章精子の分化、新編精子学(毛利秀雄・星元紀監修) (2006)
  • Author(s): 安部眞一
  • Total Pages: 55-73
  • Publisher: 東京大学出版会