2007 Fiscal Year Final Research Report Summary
Comprehensive understanding of the multifunctional GnRH peptidergic nervous system
| Project/Area Number |
18370029
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Animal physiology/Animal behavior
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
OKA Yoshitaka The University of Tokyo, Graduate School of Science, Professor (70143360)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKAZOME Yasuhisa The University of Tokyo, Graduate School of Science, Assistant professor (50302807)
ABE Hideki The University of Tokyo, Graduate School of Science, Assistant professor (90396804)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | Peptide Neuron / Exocytosis / Neuromodulation / Molecular Physiology / Calcium Ion / Imaging / Patch Clamp / GnRH |
|---|
| Research Abstract |
Sensor inputs to the TN-GnRH neurons: The environmental changes impinge upon the sensory organs of the animals and then are processed by the sensory processing mechanisms of the brain. Here, we analyzed the synaptic inputs to the TN-GnRH neurons that arise from the sensory processing brain areas. We obtained electrophysiological evidence to show the occurrence of depolarizing GABAergic synaptic inputs to the TN-GnRH neurons, which may play an important role in the transmission of sensory inputs to the neuromodulatory TN-GnRH neurons.
Isolated primary culture of TN-GnRH neurons : We newly developed a method to culture isolated TN-GnRH neurons, which should enable us to reconstitute three dimensional structures of the GnRH neurons in a two-dimensional primary culture system. We plan to use this new preparation for the simultaneous recording of intracellular calcium dynamics (calcium imaging), neural-activities (electrophysiology), and exocytotic GnRH release (carbon fiber amperometry and membrane capacitance measurement).
Generation and analysis of transgenic medaka fish: We first generated a transgenic medaka line in which three morphologically as well as functionally different GnRH neurons express GFP fluorescent proteins. Using one of such transgenic medaka fish, gnrh2-GFP transgenic medaka fish, we could record spontaneous electrical activities of the gnrh2 neurons for the first time in the vertebrate brains. We showed that they exhibit spontaneous regular pacemaker activities similar to the neuromodulatory TN-GnRH neurons, which may suggest that the spontaneous regular pacemaker activity may represent neuromodulatory functions. We also succeeded in recording the electrical activities of the hypophysiotropic GnRH neurons (gneh1 neurons) from gnrh1-GFP transgenic medaka, which showed irregular spontaneous activities quite different from the neuromodulatory gnrh2 and gnrh3 neurons.
|
-
[Journal Article] Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually-dimorphic kisspeptin neurons in medaka (Oryzias latipes)2008
Author(s)
Kanda, S., Akazome, Y., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., and Oka, Y.
-
Journal Title
Endocrinology 149
Pages: 2467-2476
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Immunohistochemical localization of GnRH-like peptide in the brain of cephalopod, spear squid Loligo bleekeri.2008
Author(s)
Amano, M., Oka, Y., Nagai, Y., Amiya, N., and Yamamori, K.
-
Journal Title
General and Comparative Endocrinology 156
Pages: 277-284
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
-
[Journal Article] Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually-dimorphic kisspeptin neurons in medaka (Oryzias latipes)2008
Author(s)
Kanda, S., Akazome, Y., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., Oka, Y.
-
Journal Title
Ebdicrinology 149
Pages: 2467-2476
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Immunohistochemical localization of a GnRH-like peptide in the brain of the cephalopod spear-squid, Loligo bleekeri2008
Author(s)
Amano, M., Oka, Y., Nagai, Y., Amiya, N., Yamamori, K.
-
Journal Title
General and Comparative Endocrinol 156
Pages: 277-284
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] Terminal nerve GnRH neurons express multiple GnRH receptors in a teleost, the dwarf gourami (Colisa lalia).2007
Author(s)
Hajdu, P., Ikemoto, T., Akazome, Y., Park, M. K., & Oka, Y.
-
Journal Title
Journal of Neuroendocrinology 19
Pages: 475-479
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Immunohistochemical localization of orexin/hypocretin-like immunoreactive peptides in the brain ahd pituitary of medaka.2007
Author(s)
Amiya, N., Amano, M., Oka, Y., Iigo, M., Takahashi, A., and Yamamori, K.
-
Journal Title
Neuroscience Letters 427
Pages: 16-21
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
-
-
-
-
-
-
[Journal Article] Terminal nerve GnRH neurons express multiple GnRH receptors in a teleost, the dwarf gourami (Colisalalia)2007
Author(s)
Hajdu, P., Ikemoto, T., Akazome, Y., Park, M. K., Oka, Y.
-
Journal Title
Journal of Neuroendocrinology 19
Pages: 475-479
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Immunohistochemical localization of orexin/hypocretin-like immunoreactive peptides in the brain and pituitary of medaka2007
Author(s)
Amiya, N., Amano, M., Oka, Y., Iigo, M., Takahashi, A., Yamamori, K.
-
Journal Title
Neuroscience Letters 427
Pages: 16-21
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Sexually dimorphic metastin neurons in medaka brain2007
Author(s)
Matsumoto, S., Akazome, Y., Yamamoto, N., Okubo, K., Yamada, S., Tsukamura, H., Maeda, K., Oka, Y.
Organizer
The 30th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Yokohama
Year and Date
20070910-12
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Possible novel function of Metastin neurons as dual neuromodulators in the vertebrate brain2006
Author(s)
Matsumoto, S., Yamamoto, N., Y. Akazome, Yamada, S., Tsukamura, H., Maeda, K., Oka, Y.
Organizer
The 77th Annual Meeting of the Zoological Society of Japan
Place of Presentation
Matsue
Year and Date
20060921-24
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] Orexin neurons in fish brain2006
Author(s)
Amiya, N., Sunuma, T., Amano, K., ligo, M., Kitamura, S., Oka, Y., Takahashi, A., Yamamori, K.
Organizer
The 31st Annual Meeting of the Japan Society for Comparative Endocrinology
Place of Presentation
Sapporo
Year and Date
20060903-04
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Immunohistochemical localization of prolactin-releasing peptide in the brain of the ovoviviparous fish species Poecilia reticulata (guppy)2006
Author(s)
Amano, K., Amiya, N., Matsuki, M., Ito, H., Oka, Y., Yamamori, K.
Organizer
The 31st Annual Meeting of the Japan Society for Comparative Endocrinology
Place of Presentation
Sapporo
Year and Date
20060903-04
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
