Origin of tripartite tubular mastigonemes and phylogeny of the Chromista lineage deduced from mastigoneme gene divergence

2008 Fiscal Year Final Research Report

• Project/Area Number: 18370037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Kobe University
• Principal Investigator: KAWAI Hiroshi Kobe University, 自然科学系先端融合研究環内海域環境教育研究センタ, 教授 (30161269)
• Co-Investigator(Kenkyū-buntansha): YAMAGISHI Takahiro 神戸大学, 自然科学系先端融合研究環重点研究部, 助教 (30379333) ; MOTOMURA Taizo 北海道大学, 北方生物圏フィールド科学センター, 教授 (30183974) ; NAKAYAMA Takeshi 筑波大学, 大学院・生命環境科学研究科, 講師 (40302369)
• Project Period (FY): 2006 – 2008
• Keywords: 不等毛藻類 / 黄金色藻 / クロミスタ / マスチゴネマ / 新規タンパク質 / ストラメノパイル / オクロモナス / 褐藻

Research Abstract

黄金藻Ochromonas danicaから単離した鞭毛からレクチンにより管状マスチゴネマの単離・精製を行い、構成タンパク質のアミノ酸配列を解析した。得られたアミノ酸配列から抗体を作製し、蛍光抗体法によりその局在部位を解析するとともに、DNA 塩基配列を決定しマスチゴネマ構成タンパク質Ocm1~4の遺伝子を決定した。またこの遺伝子情報によりストラメノパイル系統群の系統解析を行ったほか、遺伝子導入系の確立に向けた検討を行った。

Research Products

• [Journal Article] Novel proteins composing the stramenopile tripartite mastigoneme in Ochromonas danica (Chrysophyceae) (2009)
  • Author(s): Yamagishi, T., Motomura, T., Nagasato, C., Kawai, H
  • Journal Title: J. Phycol (In press)
  • Peer Reviewed
• [Journal Article] Nuclearia thermophila sp. nov. (Nucleariidae) (2009)
  • Author(s): Yoshida, M., Nakayama, T. Inouye, I
  • Journal Title: a new nucleariid species isolated from Yunoko Lake in Nikko (Japan)(European Journal of Protistology) (in press)
  • Peer Reviewed
• [Journal Article] 2009. The introduction to Japan of the Titan barnacle (2009)
  • Author(s): Yamaguchi, T., Prabowo, R. E., Ohshiro, Y., Shimono, T., Jones, D., Kawai, H., Otani, M., Oshino, A., Inagawa, S., Akaya, T., Tamura, I
  • Journal Title: Megabalanus coccopoma (Darwin, 1854) (Cirripedia : Balanomorpha) and the role of shipping in its translocation(Biofouling) (In press)
  • Peer Reviewed
• [Journal Article] Diaphragm development in cytokinetic vegetative cells of brown algae (2009)
  • Author(s): Katsaros, C., Motomura, T., Nagasato, C., Galatis, B
  • Journal Title: Bot. Mar 52 Pages: 150-161
  • Peer Reviewed
• [Journal Article] Ultrastructure of mitosis and cytokinesis during spematogenesis in Porphyra yezoensis (Bangiales, Rhodophyta) (2009)
  • Author(s): Ueki, C., Nagasato, C., Motomura, T., Saga, N
  • Journal Title: Bot. Mar 52 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Effect of latrunculin B and brefeldin A on cytokinesis in the brown alga, Scytosiphon lomentaria(Scytosiphonales, Phaeophyceae) (2009)
  • Author(s): Nagasato, C., Motomura, T
  • Journal Title: J. of Phycol 45 Pages: 404-412
  • Peer Reviewed
• [Journal Article] C. Functional and non-functional spindle formation affecting mitosis and cytokinesis in Fucus distichus zygotes : the role of the centrosome (2009)
  • Author(s): Motomura, T., Nagasato
  • Journal Title: Bot. Mar 52 Pages: 140-149
  • Peer Reviewed
• [Journal Article] Genetic diversity and introgression in two cultivated species(Porphyra yezoensis and P. tenera)and closely related wild species of Porphyra(Bangiales, Rhodophyta) (2009)
  • Author(s): Niwa, K., Iida, S., Kato, A., Kawai, H., Kikuchi, N., Kobiyama, A., Aruga, Y
  • Journal Title: J. Phycol 45 Pages: 493-502
  • Peer Reviewed
• [Journal Article] Geographical genetic structure and phylogeography of the Sargassum horneri/filicinum complex in Japan, based on the mitochondrial cox3 haplotype (2009)
  • Author(s): Uwai, S., Kogame, K., Yoshida, G., Kawai, H., Ajisaka, T
  • Journal Title: Marine Biology 156 Pages: 901-11
  • Peer Reviewed
• [Journal Article] Characterization of the crustose red alga Peyssonnelia japonica (Rhodophyta, Gigartinales) and its taxonomic relationship with P. boudouresquei based on morphological and molecular data (2009)
  • Author(s): Kato, A., Guimaraes, S.M.P.B., Kawai, H., Masuda, M
  • Journal Title: Phycol. Res 57 Pages: 74-86
  • Peer Reviewed
• [Journal Article] Reexamination of the Pit Plugs and the Characteristic Membranous Structures in Porphyra Yezoensis (Bangiales, Rhodophyta) (2008)
  • Author(s): Ueki, C., Nagasato, C., Motomura, T. and Saga, N
  • Journal Title: Phycologia 47 Pages: 5-11
  • Peer Reviewed
• [Journal Article] New records of Padina species from the western coast of the Pacific Ocean (2008)
  • Author(s): Ni-Ni-Win, Hanyuda, T., Arai, S., Uchimura, M., Abbott, I.A., Kawai, H
  • Journal Title: Phycol. Res 56 Pages: 288-300
  • Peer Reviewed
• [Journal Article] 2008. Morphology and molecular phylogeny of Aureophycus aleuticus gen. et sp. nov. (Laminariales, Phaeophyceae) from the Aleutian Islands (2008)
  • Author(s): Kawai, H., Hanyuda, T., Lindeberg, M., Lindstrom, S.C
  • Journal Title: J. Phycol 44 Pages: 1031-12
  • Peer Reviewed
• [Journal Article] 2008. Recurrent invation of mitochondrial group II introns in specimens of Pylaiella littoralis(brown alga), collected worldwide (2008)
  • Author(s): Ikuta, K., Kawai, H., Muller, D.G., Ohama, T
  • Journal Title: Current Genetics 53 Pages: 2007-16
  • Peer Reviewed
• [Journal Article] 2008. Disposal of seaweed wakame(Undaria pinnatifida) in composting process by marine bacteria Halomonas sp. AW4. Int. J (2008)
  • Author(s): Tang, J.C., Xiao Y., Oshima, A., Kawai, H., Nagata, S
  • Journal Title: Biotechnology 10 Pages: 73-85
  • Peer Reviewed
• [Journal Article] 2008. Comparative study on wild and cultivated Porphyra yezoensis (Bangiales, Rhodophyta) based on molecular phylogeny and morphology (2008)
  • Author(s): Niwa, K, Kato, A., Kobiyama, A., Kawai, H., Aruga, Y
  • Journal Title: J. Appl. Phycol 20 Pages: 261-70
  • Peer Reviewed
• [Journal Article] Gamagori City Office. Species diversity and seasonal changes of dominant Ulva species(Ulvales, Ulvophyceae) in Mikawa Bay (2007)
  • Author(s): Kawai, H., Shimada, S., Hanyuda, T., Suzuki, T.
  • Journal Title: Japan deduced from ITS2 rDNA region sequences 22 Pages: 222-30
  • Peer Reviewed
• [Journal Article] A new species of Dactylopusioides(Copepoda : Harpacticoida : Thalestridae)infesting brown algae, and its life history (2007)
  • Author(s): Shimono, T., Iwasaki, N., Kawai, H
  • Journal Title: Zootaxa 1582 Pages: 59-68
  • Peer Reviewed
• [Journal Article] Genetic diversity of Scytosiphon lomentaria(Scytosiphonaceae, Phaeophyceae)from the Pacific and Europe based on RuBisCO large subunit and spacer, and ITS nrDNA sequences (2007)
  • Author(s): Cho, G.Y., Kogame, K., Kawai, H., Boo, S.M
  • Journal Title: Phycologia 46 Pages: 657-65
  • Peer Reviewed
• [Journal Article] Molecular phylogeny of crustose brown algae (Ralfsiales, Phaeophyceae) inferred from rbcL sequences resulting in proposal for Neoralfsiaceae fam.nov (2007)
  • Author(s): Lim, P.E., Sakaguchi, M., Hanyuda, T., Kogame, K., Phang, S.-M., Kawai, H
  • Journal Title: Phycologia 46 Pages: 456-66
  • Peer Reviewed
• [Journal Article] New record of Sargassum filicinum Harvey (Fucales, Phaeophyceae) in the Pacific Coast of Mexico (2007)
  • Author(s): Aguilar-Rosas, L.E., Aguilar-Rosas, R., Kawai, H., Uwai, S., Valenzuela-Espinoza. E
  • Journal Title: 22 Pages: 17-21
  • Peer Reviewed
• [Journal Article] Marine Research Group of Takeno. Ten years' monitoring of intertidal macroalgal vegetation of Hyogo Prefecture, northwestern coast of Honshu (2007)
  • Author(s): Kawai, H., Kamiya, M., Komatsu, T., Nakaoka, M., Yamamoto, T.
  • Journal Title: Japan to assess the impact of the Nakhodka oil spill 22 Pages: 37-44
  • Peer Reviewed
• [Journal Article] Occurrence and diversity of barnacles on international ships visiting Osaka Bay, Japan, and their risk of introduction (2007)
  • Author(s): Ohtani, M., Ohmi, T., Uwai, S., Hanyuda, T., Prabowo, R.E., Yamaguchi, T., Kawai, H
  • Journal Title: Biofouling 23 Pages: 277-86
  • Peer Reviewed
• [Journal Article] Genetic distinctness and phylogenetic relationships among Undaria spp. based on mitochondrial cox3 sequence (2007)
  • Author(s): Uwai, S., Arai, S., Morita, T., Kawai, H
  • Journal Title: Phycol 45 Pages: 687-95
  • Peer Reviewed
• [Journal Article] A tubular mastigoneme-related protein ocm1 isolated from the flagellum of a chromophyte alga Ochromonas danica (2007)
  • Author(s): Yamagishi, T., Motomura, T., Nagasato, C., Kato, A., Kawai, H
  • Journal Title: J. Phycol 43 Pages: 519-27
• [Journal Article] Reexamination of ultrastructures in the stellate chloroplasts of brown algae ; Splachnidium rugosum, Scytothamnus australis, Asteronema ferruginea and Asterocladon interjectum (Phaeophyceae) (2007)
  • Author(s): Tanaka, A., Nagasato, C., Uwai, S., Motomura, T., Kawai, H
  • Journal Title: Phycol 55 Pages: 203-13
  • Peer Reviewed
• [Journal Article] Variation in metal concentrations in the brown alga Undaria pinnatifida in Osaka Bay, Japan (2007)
  • Author(s): Yamada, M., Yamamoto, K., Ushihara, Y., Kawai, H
  • Journal Title: Phycol 55 Pages: 222-30
  • Peer Reviewed
• [Journal Article] Degradation of seaweed wakame(Undaria pinnatifida)by composting process with inoculation of Bacillus sp. HR6 (2007)
  • Author(s): Tang, J.C., Wei, J.-H., Maeda, K., Kawai, H., Zhou, Q., Hosoi-Tanabe, S., Nagata, S
  • Journal Title: Biocontrol Science 12 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Cladosiphon umezakii sp. nov.(Ectocarpales, Phaeophyceae)from Japan (2007)
  • Author(s): Ajisaka, T., Kim, S.-H., Uwai, S., Kawai, H
  • Journal Title: Phycol 55 Pages: 203-13
  • Peer Reviewed
• [Journal Article] First report of the Asian seaweed Sargassum filicinum Harvey(Fucales)in California, USA (2007)
  • Author(s): Miller, K. A., Engle J., Uwai S., Kawai, H
  • Journal Title: Biol. Invasions 9 Pages: 609-13
  • Peer Reviewed
• [Journal Article] Molecular phylogeny of Discosporangium mesarthrocarpum (Phaeophyceae) with a reassessment of the order Discosporangiales (2007)
  • Author(s): Kawai, H., Hanyuda, T., Draisma, S.G.A., Muller, D.G
  • Journal Title: J. Phycol 43 Pages: 186-94
  • Peer Reviewed
• [Journal Article] Taxonomic revision of the genus Chorda(Chordaceae, Laminariales)based on sporophyte anatomy and molecular phylogeny (2007)
  • Author(s): Sasaki, H., Kawai, H
  • Journal Title: Phycologia 46 Pages: 10-21
  • Peer Reviewed
• [Journal Article] Ultrastrucrural and immunocytological studies on the rhizoplast in the chrysophycean alga Ochromonas danica (2006)
  • Author(s): Uemori, C., Nagasato, C., Kato, A. and Motomura, T
  • Journal Title: Phycol 54 Pages: 133-9
  • Peer Reviewed
• [Journal Article] Reassessment of the little-known crustose red algal genus Polystrata Heydrich(Gigartinales), based on morphology and SSU rDNA sequences (2006)
  • Author(s): Kato, A., Baba, M., Kawai, H., Masuda, M
  • Journal Title: J. Phycol 42 Pages: 922-33
  • Peer Reviewed
• [Journal Article] 2006. Genetic diversity in Undaria pinnatifida(Laminariales, Phaeophyceae)deduced from mitochondria genes-origins and succession of introduced populations (2006)
  • Author(s): Uwai, S., Nelson, W., Neill, K., Wang, W. D., Aguilar-Rosas, L.E., Boo, S.M., Kitayama, T., Kawai, H
  • Journal Title: Phycologia 45 Pages: 687-95
  • Peer Reviewed
• [Journal Article] Utilization of proline in Escherichia coli K-12 at different osmolarities (2006)
  • Author(s): Sasaki, H., Ishida, A., Hashimoto, Y., Takeda, S., Oshima, A. Kawai, H., Nagata, S
  • Journal Title: J. Biol. Sci 6 Pages: 675-9
  • Peer Reviewed
• [Journal Article] A survey of organic solvent extractable metal concentrations in the bottom sediment in Osaka Bay, Japan (2006)
  • Author(s): Yamada, M., Yamamoto, K., Ushihara, Y., Kawai, H
  • Journal Title: Mar. Poll. Bull 52 Pages: 214-38
  • Peer Reviewed
• [Journal Article] Biochemical phenotypes corresponding to molecular phylogeny of the red algae Plocamium(Plocamiales, Rhodophyta) : implications of incongruence with the conventional taxonomy (2006)
  • Author(s): Yano, T., Kamiya, M., Murakami, A., Sasaki, H., Kawai, H
  • Journal Title: J. Phycol 42 Pages: 155-69
  • Peer Reviewed
• [Presentation] Macroalgal cryopreservation in Kobe University macroalgal culture collection(KU-MACC) (2008)
  • Author(s): Kawai H. and Yamagishi T
  • Organizer: Algal Culture Collection 2008
  • Place of Presentation: Oban, Scotland, UK
  • Year and Date: 20080608-11
• [Presentation] Structural diversity of the EcOcm3 gene introns in Ectocarpus spp. Ectocarpus 2008 (2008)
  • Author(s): Yamagishi T., Hanyuda T., Takagi R., Muller D. G. and Kawai, H
  • Organizer: Ectocarpus 2008
  • Place of Presentation: Oban, Scotland, UK
  • Year and Date: 20080604-08
• [Presentation] Flagella-related gene annotation (2008)
  • Author(s): Yamagishi T. and Kawai H
  • Organizer: Ectocarpus genome project annotation meeting
  • Place of Presentation: Oban, Scotland, UK
  • Year and Date: 20080603-04
• [Presentation] Identification of the proteins composing the tripartite tubular mastigoneme in a chromophyta alga, Ocromonas danica (2007)
  • Author(s): Yamagishi, T., Motomura, T., Nagasato, C., Kawai, H
  • Organizer: 4^<th> European Phycological Congress
  • Place of Presentation: Oviedo, Spain
  • Year and Date: 20070723-27
• [Presentation] 2006. Molecular phylogeny of Discosporangium mesarthrocarpum (Phaeophyceae) with a reassessment of the order Discosporangiales (2006)
  • Author(s): Hanyuda, T., Drasima, S.G., Muller, D.G
  • Organizer: The 2006 annual meeting of Phycological Society of America
  • Place of Presentation: Juneau, Alaska, U.S.A.
  • Year and Date: 20060707-12