2009 Fiscal Year Final Research Report
Characterization of stress responses and its application to the lifespan regulation in model microorganisms.
| Project/Area Number |
18370070
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
AIBA Hirofumi Nagoya University, 大学院・生命農学研究科, 准教授 (60211687)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 2成分制御系 / 経時寿命 / 大腸菌 / 分裂酵母 |
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| Research Abstract |
To understand the strategy of stress responses of microorganisms, I characterized two model microorganisms, E.coli and S.pombe. In E.coli, I found that a response regulator, ArcA is involved in fragella synthesis in addition to the well known aerobic-anaerobic responses. I also identified the whole genome binding sites of transcription factors, ArcA, H-NS and Fnr of E. coli by using ChIP-CHIP analysis.
By using fission yeast, S pombe, I started to identify and characterize factors involved in the chronological lifespan. In this project, I identified novel factors, Ecll family proteins that increase chronological lifespan of S. pombe when overexpressed. I also isolated some mutants and clones that affect chronological lifespan of S pombe. Based on these results, I was able to establish the basis for the development of the future research.
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[Journal Article] The implication of YggT of Escherichia coli in osmotic regulation.2009
Author(s)
Ito. T., Uozami, N., Nakamura, T., Takayama, S., Matsuda, N., Aiba, H., Hemmi, H., Yoshimura, T.
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Journal Title
Biosci. Biotech. Biochem 73
Pages: 2698-2704
Peer Reviewed
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[Journal Article] Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast.2009
Author(s)
Shimada, M., Yamamoto, A., Murakami Tonami, Y., Nakanishi, M., Yoshida, T., Aiba, H., Murakami, H.
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Journal Title
Biochem. Biophy. Res. Com. 388
Pages: 529-532
Peer Reviewed
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[Journal Article] A novel gene, ecll+, extends chronological lifespan in fission yeast.2008
Author(s)
Ohtsuka, H., Mita, S., Ogawa, Y., Azuma, K., Ito, H., Aiba, H.
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Journal Title
FEMS Yeast Research 8
Pages: 520-530
Peer Reviewed
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