• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

Analysis of the mechanism of septin polymerization and depolymerization

Research Project

Project/Area Number 18370077
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKyoto University

Principal Investigator

KINOSHITA Makoto  Kyoto University, Graduate School of Medicine, lecturer (30273460)

Project Period (FY) 2006 – 2007
Keywordsbiomolecule / protein / cell / tissue / brain / nervous system / cytoskeleton / reconstirution / live imaging / knockout mouse
Research Abstract

Microtubule and actin cytoskeleton provide infrastructure for neuronal and glial morphology and functions, whereas another ubiquitous nucleotide-binding cytoskeleton composed of septins is poorly characterized despite their abundance in the brain and implications in synaptic transmission and neurodegeneration. Here we find diverse submembranous septin clusters in vivo and analyze their dynamic properties and functions in vitro. Stereoscopic reconstruction of serial immunoelectron micrographs of the mouse brain reveals numerous septin clusters that contain brain-specific subunits. In the cerebellum, Sept4-containing septin heteropolymers cluster beneath concave membrane regions of Bergmann glial processes each surrounds a dendritic spine neck of a Purkinje cell, while Sept5-containing clusters preferentially underlie dendritic shafts up to spine bases in Purkinje cells. In the hippocampus, Sept3-containing clusters localize beneath mossy fiber terminals that intricately wrap around pyramidal cell dendrites. The unique distribution of septin clusters should reflect their as yet unidentified roles beneath specific extra/para-synaptic membrane domains in neurons and glia. As an in vitro approach to address this, we examine temporal properties of septins and related molecules underlying neuroblastoma cell membrane by fluorescence recovery after photobleaching (FRAP) analysis. The remodeling dynamics of cortical septin assembly is intrinsically slow, which is facilitated by actin turnover. While RNAi-mediated septin depletion does not significantly affect cortical actin dynamics, it enhances the diffusional mobilities of membrane proteins. Together, the submembranous septin clusters found in vivo may serve as scaffolds and/or diffusion barriers that biochemically tether and/or physically restrain a specific subset of membrane-proximal molecules for the functional compartmentalization of specialized membrane subdomains.

  • Research Products

    (10 results)

All 2008 2007 2005 Other

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Morphogenesis of polarized epithelia requires Sept2-mediated coupling of vesicle transport to polyglutamylated microtubules.2008

    • Author(s)
      Spiliotis ET, Kinoshita M, 他3名
    • Journal Title

      Journal of Cell Biology 180

      Pages: 295-303

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Loss of Sept4 exacerbates liver fibrosis through the dysregulation of hepatic stellate cells.2008

    • Author(s)
      Iwaisako K, Kinoshita M, 他8名
    • Journal Title

      Journal of Hepatology 未定(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Morphogenesis of polarized epithelia requires Sept2-mediated coupling of vesicle transport to polyglutamylated microtubules2008

    • Author(s)
      Spiliotis, ET, Hunt, S., Hu, Q., Kinoshita, M., Nelson, WJ
    • Journal Title

      Journal of Cell Biology 180

      Pages: 295-303

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sept4, a component of presynaptic scaffold and Lewy bodies, is required for the suppression of α-synuclein neurotoxicity.2007

    • Author(s)
      Ihara M, Kinoshita M, 他11名
    • Journal Title

      Neuron 53

      Pages: 519-533

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Sept4, a component of presynaptic scaffold and Lewy bodies, is required for the suppression of a-synuclein neurotoxicity2007

    • Author(s)
      Ihara, M., Yamasaki, N., Hagiwara, A., Tomimoto, H., Kitano, A., Tanigaki, A., Hikawa, E., Noda, M., Takanashi, M., Mori, H., Hattori, N., Miyakawa,T., Kinoshita, M
    • Journal Title

      Neuron 53

      Pages: 519-533

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Loss of Sept4 exacerbates liver fibrosis through the dysregulation of hepatic stellate cells

    • Author(s)
      Iwaisako, K., Hatano, E., Taura, K., Nakajima, A., Tada, M., Seo, S., Tamaki, N., Ikai, I., Uemoto, S., Kinoshita, M
    • Journal Title

      Journal of Hepatology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Insight into septin functions from mouse models

    • Author(s)
      Kinoshita, M
    • Journal Title

      In The Septins (eds. J.Pringle, and P. Hall) John Wiley & Sons (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Sept4, a component of presynaptic scaffold and Lewy bodies, is required for the suppression of alpha-synuclein neurotoxicity.2007

    • Author(s)
      Kinoshita M
    • Organizer
      EMBO Workshop/The 2nd International Septin Workshop. Keynote Lecture
    • Place of Presentation
      Ascona, Switzerland
    • Year and Date
      20070506-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Sept4, a component of presynaptic scaffold and Lewy bodies, is required for the suppression of alpha-synuclein neurotoxicity2005

    • Author(s)
      Kinoshita, M., Keynote, Lecture
    • Organizer
      EMBO Workshop/The 2" International Septin Workshop
    • Place of Presentation
      (Ascona, Switzerland)
    • Year and Date
      20050506-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Insight into septin functions from mouse models. In The Septions(eds. S. E. H. Russell, J. Pringle, and P. Hall)2008

    • Author(s)
      kinoshita M
    • Publisher
      John Wiley & Sons(印刷中)
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi