Studies on the mechanisms of left-right asymmetric development involving actin cytoskeleton and unconventional myosins inDrosophila

2008 Fiscal Year Final Research Report

• Project/Area Number: 18370091
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo University of Science
• Principal Investigator: MATSUNO Kenji Tokyo University of Science, 基礎工学部, 教授 (60318227)
• Co-Investigator(Renkei-kenkyūsha): MAEDA Reo 東京理科大学, 基礎工学部, 助教 (40453831) ; 奥村 高志 東京理科大学, 基礎工学部, 特別研究員 (20449234)
• Project Period (FY): 2006 – 2008
• Keywords: 左右非対称性 / 消化管 / 上皮細胞 / ミオシン / アクチン / E-カドヘリン / ショウジョウバエ / シミュレーション

Research Abstract

動物のからだが左右非対称になる機構は、進化的に多様であり、脊椎動物の一部を除いて、ほとんど理解されていない
本研究では、遺伝学的解析手段が駆使できるショウジョウバエの消化管にみられるステレオタイプな左右非対称性に着目した。胚消化管の左右性に異常を示す突然変異体を網羅的に検索し、得られた遺伝子の機能を解析した。その結果、Myosin31DFが、DE-cadherinの機能を介して、消化管上皮細胞の形態を左右非対称にしていることがわかった。コンピュータ・シミュレーションによって、この左右非対称な形状が、左右非対称性形成に十分であることを示した

Research Products

• [Journal Article] The development and evolution of left-right asymmetry ininvertebrates : lessons fromDrosophila and snails (2008)
  • Author(s): Okumura, T., Utsuno, H., Kuroda, J., Gittenberger, E., Asami, T., andMatsuno, K.
  • Journal Title: Dev. Dyn. 237 Pages: 3497-3515
• [Journal Article] The head region of unconventional Myosin I familymembers is responsible for theorgan-specificity of their roles in left-right polarity in Drosophila (2008)
  • Author(s): Hozumi, S., Maeda, R., Taniguchi-Kanai, M., Okumura, T., Taniguchi, K., Kawakatsu, Y., Nakazawa, N., Hatori, R., and Matsuno, K.
  • Journal Title: Dev. Dyn. 237 Pages: 3528-3537
• [Journal Article] Drosophila HOPS and AP-3 complex genes are required for aDeltex-regulated activation of Notchin the endosomal trafficking pathway (2008)
  • Author(s): Wilkin, M. B., Tongngok, P., Gensch, N., Clemence, S., Motoki, M., Yamada, K., Hori, K., Taniguchi-Kanai, M., Franklin, E., Matsuno, K., and Baron, M.
  • Journal Title: Dev. Cell 15 Pages: 762-772
• [Journal Article] Distinct activation patterns of EGF receptor signaling inthe homoplastic evolution of eggshellmorphology in genus Drosophila (2008)
  • Author(s): Kagesawa, T., Nakamura, Y., Nishikawa, M., Akiyama, Y., Kajiwara, M., andMatsuno, K.
  • Journal Title: Mech. Dev. 125 Pages: 1020-1032
• [Journal Article] The O-fucosyltransferase O-fut1 is an extracellular component that isessential for the constitutiveendocytic trafficking of Notch inDrosophila (2007)
  • Author(s): Sasamura, T., Ishikawa, H. O., Sasaki, N., Higashi, S., Kanai, M., Nakao, S., Ayukawa, T., Aigaki, T.,Noda, K., Miyoshi, E., Taniguchi, N., and Matsuno, K.
  • Journal Title: Development 134 Pages: 1347-1356
• [Journal Article] Soma-dependent modulations contribute to divergence of rhomboid expressionduring evolution of Drosophilaeggshell morphology (2007)
  • Author(s): Nakamura, Y., Kagesawa, T., Nishikawa, M., Hayashi, Y., Kobayashi, S., Niimi, T., andMatsuno, K.
  • Journal Title: Development 134 Pages: 1529-1537
• [Journal Article] Roles of type I Myosins in Drosophila handedness (2007)
  • Author(s): Taniguchi, K., Hozumi, S., Maeda, R., Okumura, T., andMatsuno, K.
  • Journal Title: Fly 1 Pages: 287-290
• [Journal Article] D-JNK signaling in visceral muscle cells controls the laterality of theDrosophila gut (2007)
  • Author(s): Taniguchi, K., Hozumi, S., Maeda, R., Ooike, M., Sasamura, T., Aigaki, T., and Matsuno, K.
  • Journal Title: Dev. Biol. 311 Pages: 251-263
• [Journal Article] Mastermind-1 is required for Notch signal-dependent steps in lymphocytedevelopment in vivo (2007)
  • Author(s): Oyama, T., Harigaya, K., Muradil, A., Hozumi, K., Habu, S., Oguro, H., Iwama, A., Matsuno, K., Sakamoto, R., Sato M.,Yoshida, N., and Kitagawa, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 9764-9769
• [Journal Article] The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport (2007)
  • Author(s): Araki, Y., Kawano, T., Taru, H., Saito, Y., Wada, S., Miyamoto, K., Kobayashi, H., Ishikawa, H. O., Ohsugi, Y., Yamamoto, T., Matsuno, K., Kinjo, M., and Suzuki, T.
  • Journal Title: EMBO J 26 Pages: 1475-1486
• [Journal Article] Roles of single-minded in the left-right asymmetric development andtissue specifications of theDrosophila embryonic gut (2007)
  • Author(s): Maeda, R., Hozumi, S., Taniguchi, K., Sasamura, T., Murakami, R., and Matsuno, K.
  • Journal Title: Mech. Dev. 124 Pages: 204-217
• [Journal Article] Polarized exocytosis and transcytosis of Notch during itsapical localization in Drosophilaepithelial cells (2007)
  • Author(s): Sasaki N., Sasamura, T., Ishikawa, H. O., Kanai, M., Ueda, R., Saigo, K., Aigaki, T., and Matsuno K.
  • Journal Title: Genes to Cells 12 Pages: 89-103
• [Journal Article] ショウジョウバエにおける左右非対称性形成の遺伝学的解析 (2007)
  • Author(s): 前田礼男, 穂積俊矢, 谷口喜一郎, 奥村高志, 松野健治
  • Journal Title: 生化学 6(12) Pages: 1131-1134
• [Journal Article] The first deltex null mutant indicates tissue-specific deltex-dependentNotch signaling in Drosophila (2006)
  • Author(s): Fuwa, T. J., Hori, K., Sasamura, T., Higgs, J., Baron, M. and Matsuno, K.
  • Journal Title: Mol. Genet. and Genom. 275 Pages: 251-263
• [Journal Article] A Drosophila unconventional myosin reverses the defaulthandedness in visceral organs (2006)
  • Author(s): Hozumi, S., Maeda, R., Taniguchi, K., Sasamura, T., Speder, P., Noselli, S., Aigaki, T., Murakami, R. and Matsuno, K.
  • Journal Title: Nature 440 Pages: 798-802
• [Presentation] ショウジョウバエ胚の消化管における左右非対称な発生に関わる遺伝子のスクリーニング (2008)
  • Author(s): Naotaka Nakazawa, Kiichiro Taniguchi, Takashi Okumura, Reo Maeda, SyunyaHozumi, Mitutoshi Nakamura, HarukaYamamoto, Takeshi Kusama, Ryo Hatori, kenji Matsuno
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20080528-29
• [Presentation] DE-Cadherin-and MyosinID-dependent cell shapechanges were involved in thelaterality of the Drosophilaembryonic gut. (2008)
  • Author(s): Kiichiro Taniguchi, Tadashi Ando, Takashi Okumura, Reo Maeda, NaotakaNakazawa, syunya Hozumi, KenjiMatsuno
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20080528-29
• [Presentation] Doctor no and Ken and Barbie are involved in theLeft-right asymmetrical developmentof the Drosophila embryonic gut. (2008)
  • Author(s): Reo Maeda, Shunya Hozumi, Kiichiro Taniguchi, Takeshi Sasamura, ToshiroAiaki, Kenji Matsuno
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20080528-29
• [Presentation] ショウジョウバエにおける左右非対称性の形成機構-遺伝学とコンピュタ・シミュレーションによるアプローチ (2008)
  • Author(s): 松野健治
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Septate junctions are required for left-right asymmetric development ofthe embryonic gut in Drosophilamelanogaster. (2008)
  • Author(s): Yasuhiro Kawakatsu, Takasi Okumura, Reo Maeda, Kiichiro Taniguchi,Naotaka Nakazawa, Kenji Matsuno
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] DE-Cadherin-and MyosinID-dependent cell shape changes were involved inthe laterality of the Drosophilaembryonic gut. (2008)
  • Author(s): Kiichiro Taniguchi, Tadashi Ando, Takashi Okumura, MaikoTaniguchi-Kanai, Naotaka Nakazawa, Reo Maeda, Mitsutoshi Nakumura,Syunya Hozumi, Kenji Matsuno
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] A nonmuscle Myosin II heavy chain, Zipper, is required for theleft-right asymmetricmorphologenesis of the anteriormidgut in Drosophila embryo. (2008)
  • Author(s): Takashi Okumura, Hiroo Fujiwara, Kiichiro Taniguchi, Reo Maeda, HozukiSyunya, Naotaka Nakazawa, MitutoshiNakamura, Haruka Yamamoto, KenjiMstsuno
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Wntシグナル構成因子であるLDL受容体をコードするショウジョウバエarrowの消化管の左右非対称性形成における機能. (2008)
  • Author(s): 黒田純平, 吉田匡, 山本遙, 奥村高志, 前田礼男, 谷口喜一郎, 川勝康弘, 中澤直高, 中村充利, 石尾彬, 尾崎あゆみ, 羽鳥僚, 松野健治
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Doctor no and Ken and Barbie are involved in theleft-right asymmetrical developmentof the Drosophila embryonic gut. (2008)
  • Author(s): Reo Maeda, Shunya Hozumi, Kiichiro Taniguchi, Takeshi Sasamura, ToshiroAigaki, Kenji Matsuno
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Nonmuscle Myosin II heavy chain Zipper isrequired for the left-rightasymmetric morphology of the anteriormidgut in Drosophila embryo. (2008)
  • Author(s): Takashi Okumura, Hiroo Fujiwara, Kiichiro Taniguchi, Reo Maeda, Syunya Hozumi, Naotaka Nakazawa, Mitsutoshi Nakamura, HarukaYamamoto, Kenji Matsuno
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-28
• [Presentation] Doctor no and Ken and Barbie are involved in the left-rightasymmetrical development of theDrosophila embryonic gut. (2008)
  • Author(s): Reo Maeda, Shunya Hozumi, Kiichiro Taniguchi, Takeshi Sasamura, ToshiroAigaki, Ryutaro Murakami, KenjiMatsuno
  • Organizer: 49th Annual Drosophila Resarch Conference
  • Place of Presentation: サンディエゴ
  • Year and Date: 2008-04-03
• [Presentation] Nonmuscle Myosin II heavy chain Zipper is required for the left-rightasymmetric morphology of theproventriculus and midgut in Drosophila embryo. (2008)
  • Author(s): Takashi Okumura, Hiroo Fujiwara, Kiichiro Taniguchi, Reo Maeda, Syunya Hozumi, Kenji Matsuno
  • Organizer: 49th Annual Drosophila Resarch Conference
  • Place of Presentation: サンディエゴ
  • Year and Date: 2008-04-03
• [Presentation] ショウジョウバエ胚消化管の左右非対称性形成におけるsingle-minded遺伝子の役割. (2007)
  • Author(s): 前田礼男, 穂積俊矢, 谷口喜一郎, 笹村剛司, 村上柳太郎, 松野健治
  • Organizer: 第40回日本発生生物学会第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 20070529-30
• [Presentation] ショウジョウバエ胚消化管の左右非対称性形成におけるsigle-minded遺伝子の役割. (2007)
  • Author(s): 前田礼男, 穂積俊矢, 谷口喜一郎, 笹村剛司, 村上柳太郎, 松野健治
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] A genetics screen to indentify genes involved inthe left-right asymmetricdevelopment of the embryonic gut inDrosophila. (2007)
  • Author(s): 吉田匡, 前田礼男, 谷口喜一郎, 奥村高志, 黒田純平, 中澤直高, 中村充利, 山本遙, 草間岳史, 松野健治
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] JNKシグナルにより制御される内臓筋細胞の編成はショウジョウバエ中腸前部の左右非対称的な屈曲に必要である. (2007)
  • Author(s): 谷口喜一郎, 穂積俊矢, 前田礼男, 大池雅, 笹村剛司, 相垣敏郎, 松野健治
  • Organizer: 第40回日本発生生物学会第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] Roles of single-minded in the left-rightasymmetric development and tissuespecifications of the Drosophilaembryonic gut. (2007)
  • Author(s): Reo Maeda, Shunya Hozumi, Kiichiro Taniguchi, Takeshi Sasamura, RyutaroMurakami, Kenji Matsuno
  • Organizer: 48th Annual Drosophila Resarch Conference
  • Place of Presentation: フィラデルフィア
  • Year and Date: 2007-03-08
• [Presentation] The rearrangement of circular visceralmusculature cells controlled by JNKsignaling is involved in the left-right asymmetric looping of the anterior-midgut in Drosophila. (2007)
  • Author(s): Kiichiro Taniguchi, Shunya Hozumi, Reo Maeda, Shuichi Shirakabe, HirooFujiwara, Takeshi Sasamura, AigakiToshiro, Kenji Matsuno
  • Organizer: 48th Annual Drosophila Resarch Conference
  • Place of Presentation: フィラデルフィア
  • Year and Date: 2007-03-08
• [Presentation] A Drosophila unconventional myosins regulate handedness invisceral organs. (2006)
  • Author(s): Shynya Hozumi, Reo Maeda, Kiichiro Taniguchi, Maiko Kanai, SyuichiShirakabe, Takeshi Sasamura, ToshiroAigaki, Ryutaro Murakami, KenjiMatsuno
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 2006-06-03
• [Presentation] The rearrangement of visceralmusculature cells controlled by JNK signaling is involved in the left-right asymmetric looping of theanterior-midgut in Drosophila. (2006)
  • Author(s): Kiichiro Taniguchi, Shunya Hozumi, Reo Maeda, Shuichi Shirakabe, HirroFujiwara Takeshi Sasamura, ToshiroAigaki, Kenji Matsuno
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 2006-06-01
• [Presentation] A genetic screen to identify genes required for left-rightasymmetry in Drosophilamelanogaster (2006)
  • Author(s): Reo Maeda, Shunya Hozumi, Kiichiro Taniguchi, Syuichi Shirakabe, HirooFujikawa Takeshi Sasamutra, ToshiroAigaki, Ryutaro Nurakami, KenjiMatsuno
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 2006-06-01
• [Presentation] ショウジョウバエ定型非筋ミオシンII重鎖Zipperは前胃の左右非対称な屈曲に必要である.
  • Author(s): 奥村高志, 藤原普夫, 谷口喜一郎, 前田礼男, 穂積俊矢, 吉田匡, 中澤尚高, 中村充利, 草間岳史, 山本遙, 松野健治
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
• [Book] 細胞工学 (2008)
  • Author(s): 奥村高志, 川勝康弘, 谷口喜一郎, 松野健治
  • Total Pages: 553-557
  • Publisher: 秀潤社
• [Book] Glycoscience Lab Manual (2008)
  • Author(s): Ayukawa, T. and Matsuno K.
  • Total Pages: 295-298
  • Publisher: Springer Verlag, Tokyo
• [Book] 蛋白質核酸酵素 (2007)
  • Author(s): 穂積俊矢, 前田礼男, 谷口喜一郎, 松野健治
  • Total Pages: 227-235
  • Publisher: 共立出版
• [Book] 蛋白質核酸酵素 (2007)
  • Author(s): 中越英樹, 松野健治
  • Total Pages: 119-125
  • Publisher: 共立出版
• [Book] 細胞工学 (2006)
  • Author(s): 穂積俊矢, 前田礼男, 谷口喜一郎, 松野健治
  • Total Pages: 642-643
  • Publisher: 秀潤社
• [Book] Bionics (2006)
  • Author(s): 前田礼男,穂積俊矢, 谷口喜一郎, 松野健治
  • Total Pages: 70-71
  • Publisher: オーム社
• [Remarks]
  • URL: http://matsuno-lab.homeip.net/frame.html