2007 Fiscal Year Final Research Report Summary
Origin and activation mechanism of an active transposition of mPingin rice
| Project/Area Number |
18380005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kyoto University |
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Principal Investigator |
OKUMOTO Yutaka Kyoto University, Graduate School of Agriculture, Associate Professor (90152438)
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| Co-Investigator(Kenkyū-buntansha) |
TANISAKA Takatoshi Kyoto University, Graduate School of Agriculture, Professor (80026591)
NAKAZAI Tetsuya Kyoto University, Graduate School of Agriculture, Lecturer (60217693)
TSUKIYAMA Takuji Kyoto Uniyersity, Graduate School of Agriculture, Assistant Professor (00423004)
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| Project Period (FY) |
2006 – 2007
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| Keywords | rice / transposon / transposable activity / mPing / QTL analysis / genus Oryza |
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| Research Abstract |
The distribution of miniature Ping (mPing) was studied in 50 wild Oryza accessions which encompassed 19 species representing nine genomes of genus Oryza. The mPing element was presented in all the accessions arid they were highly (more than 99%) similar each other. There are three major types of mPing elements; type 1 with 430bp originally found in Gimbozu (Osativa), type 2 with 430bp with 9bp base substitution al the position of 242-252, type 3 with 419bp with 11bp deletion at the position of 239-249. Type 2 and 3 are presented only in O.sativa and O.rugipogon, The wide presence of type 1 mping in all the other accession indicated mPing elements should be originated prior to the differentiation of the genus Oryza. Transposition and amplification of mPing and Ping are still underway in rice variety Gimbozu irrespective of its high copy number of mPing element (over 1000). In Gimbozu, more than 50 new transposition estimated to be occurred per plant per generation while no transposition activity of mPing was observed in Nippcnbare, To elucidate the genetic factors contributing to the transposition activity of mPing Gimbozu, F5 progeny lines of selfed F4 plants derived form the cross between Nipponbare and Gimbozu were used. The mPing transposition activity was evaluated from a number of new mPing insertions that were not presented in parental plants. Then, genetic map of Nipponbare x Gimbozu were constructed based on the insertion polymorphism of mPing (mPingSCAR marker) between two varieties. Results of a QTL analysis for the mPing activity revealed two major QTLs at chr 1 and chr4.
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[Journal Article] Dramatic amplification of a rice transposable element during recent domestication2006
Author(s)
Naito, K., E., Cho, G., Yang, M., Campbell, K., Yano, Y., Okumoto, T., Tanisaka, S., Wessler
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Journal Title
Proc.NAS USA 103
Pages: 17620-17625
Description
「研究成果報告書概要(欧文)」より
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[Presentation] mPingSCAR, markers : a resolution to saturated linkage map construction using japonica x japonica cross2007
Author(s)
Monden, Y., K., Naito, H., Saito, N., Oki, O., Ideta, T., Nakazaki, T., Tsulciyama, Y., Okumoto, T., Tanisaka
Organizer
112th Meeting of the Japanese Society of Breeding
Place of Presentation
Yamagata University, Sakata
Year and Date
2007-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Destruction of rice ubiquitin-like modifier-I, Rurml, enhances the transposition activity of transposable elements2007
Author(s)
Tamura, K., T., Tsukiyama, Y., Okumoto, H., Horibata, H., Inagaki, T., Nakazaki, T., Tanisaka
Organizer
112th Meeting of the Japanese Society of Breeding
Place of Presentation
Yamagata University, Sakata
Year and Date
2007-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Transposon display of mutations on quantitative trait induced by the endogenous active transposon, mPing in rice (Oryza saliva)2006
Author(s)
Horibata, A., K., Matsui, E., Inoue, T., Yoshihiro, H., Kawaji, M., Nakagawa, Y., Okumoto, T., Nakazaki, T., Tanisaka
Organizer
110th Meeting of the Japanese Society of Breeding
Place of Presentation
Ehime University, Matsuyama
Year and Date
2006-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Uncovering the facto having induced the burst of mPing in japonica rice cultivar Gimbozu2006
Author(s)
Naito, K., Y., Okumoto, Y., Monden, T., Nakazaki, T., Tanisaka
Organizer
110th Meeting of the Japanese Society of Breeding
Place of Presentation
Ehime University, Matsuyama
Year and Date
2006-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Destruction of Rurml (Rice Ubiquitin-like modifier-1) enhances the transposition activity of mPing2006
Author(s)
Tamura, K., N., Oki, T., Tsukiyama, Y., Okumoto, T., Nakazaki, T., Tanisaka
Organizer
110th Meeting of the Japanese Society of Breeding
Place of Presentation
Ehime University, Matsuyama
Year and Date
2006-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of Pyong-like elements in the genome of Nipponnbare and wild rice species2006
Author(s)
Oki, N., Y., Okumoto, T., Tsukiyama, K., Naito, T., Nakazaki, T., Tanisaka
Organizer
110th Meeting of the Japanese Society of Breeding
Place of Presentation
Ehime University, Matsuyama
Year and Date
2006-09-23
Description
「研究成果報告書概要(欧文)」より
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