Fundamental study on mechanisms of salt accumulation in a halophyte aimed at establishing a phytoremediation technique

2008 Fiscal Year Final Research Report

• Project/Area Number: 18380015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Saga University
• Principal Investigator: AGARIE Sakae Saga University, 農学部, 准教授 (50304879)
• Project Period (FY): 2006 – 2008
• Keywords: 野生植物資源

Research Abstract

アイスプラント(Mesembryanthemum crystallinum L.)の塩集積細胞であるブラッダー細胞の形成に関わる遺伝子として, トライコーム形成関連遺伝子及び機能未知遺伝子を単離した.液胞膜タンパク質を二次元電気泳動法で網羅的に解析し, NaCl の集積に関与するタンパク質を同定した.塩ストレスに伴うCAM 型光合成の誘導にグルタチオンが関与していることをCAM 型欠損突然変異体の解析から明らかにした.またCAM の鍵酵素であるホスホエノールピルビン酸カルボキシラーゼキナーゼ遺伝子のプロモーター領域を単離し, CAM の誘導に関与すると考えられるシス因子を推定した.

Research Products

• [Journal Article] Potential of the common ice plant (2008)
  • Author(s): Agarie, S., A. Kawaguchi, A. Kodera, H. Sunagawa, H. Kojima, A. Nose and T. Nakahara
  • Journal Title: Mesembryanthemum crystallinum as a new high-functional food as evaluated by polyol accumulation.Plant Production Science 12 Pages: 37-46
• [Journal Article] Isolation and characterization of mutants of ice plant (2008)
  • Author(s): Cushman, J.C., S. Agarie, R.L. Albion, S.M. Elliot, T. Taybi and A.M. Borland
  • Journal Title: Mesembryanthemum crystallinum, deficient in Crassulacean acid metabolism.Plant Physiology 147 Pages: 228-238
• [Journal Article] Salt tolerance, salt accumulation, and ionic homeostasis in an epidermal bladder-cell-less mutant of the common ice plant . (2007)
  • Author(s): Agarie, S., T. Shimoda, Y. Shimizu, K. Baumann, H. Sunagawa, A. Kondo, O. Ueno, T. Nakahara, A. Nose, J.C. Cushman
  • Journal Title: Mesembryanthemum crystallinum.Journal of Experimental Botany 58 Pages: 1957-1967
• [Journal Article] Effects of urea-type cytokinins on the adventitious shoots regeneration from cotyledonary node explant in the common ice plant
  • Author(s): Sunagawa, H., S. Agarie, M. Umemoto, Y.Makishi, A. Nose
  • Journal Title: Mesembryanthemum crystallinum. Plant Production Science
• [Presentation] ポリオール生成能からみたアイスプラント(Mesembryanthemmu crystallinum)の高付加価値食材としての可能性 (2007)
  • Author(s): 東江栄・川口晶子・砂川春樹・小島英・中原光久・野瀬昭博
  • Organizer: 第224回日本作物学会
  • Place of Presentation: 金沢大学
  • Year and Date: 2007-09-27
• [Presentation] Isolation and characterization of mutants of ice plant, Mesembryanthemum crystallinum, defective in Crassulacean acid metabolism (2007)
  • Author(s): Cushman, J.C., S. Agarie, R. Albion, R. French, S.M. Elliot, T. Taybi, A.M. Borland
  • Organizer: C4 and CAM : from molecular diversity to ecological convergence
  • Place of Presentation: ケンブリッジ大学(英国)
  • Year and Date: 2007-07-17
• [Presentation] Efficient Agrobacterium-mediated transformation of Mesembryanthemum crystallinum - a facultative CAM plant (2007)
  • Author(s): Agarie, S., M. Umemoto, R. Tsuji, H. Sunagawa, J.C. Cushman, A. Nose
  • Organizer: C4 and CAM : from molecular diversity to ecological convergence
  • Place of Presentation: ケンブリッジ大学(英国)
  • Year and Date: 2007-07-17
• [Patent(Industrial Property Rights)] 水耕栽培方法 (2008)
  • Inventor(s): 東江栄、野瀬昭博
  • Industrial Property Rights Holder: 佐賀大学
  • Industrial Property Number: 特許,特願2008-104632
  • Filing Date: 2008-04-14