2007 Fiscal Year Final Research Report Summary
Exploration and characterization of low-molecular compound that induces hypersensitive cell death in plants
| Project/Area Number |
18380036
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant pathology
|
|---|
| Research Institution | National Institute of Agrobiological Sciences |
|---|
Principal Investigator |
SHIGEMI Seo National Institute of Agrobiological Sciences, Plant-Microbe Interactions Research Unit, Investigator (80414910)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SETO Hideharu RIKEN, Matsumoto Molecular Insect Laboratory, Investigator (40175419)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | signal transduction / plant / physiological activity / cell death |
|---|
| Research Abstract |
Hypersensitive cell death (HCD) is a typical disease resistance response of plants against pathogen infection. Although genetical and biochemical studies have identified many factors involved in the induction of HCD, almost all of them are proteineous factors, and little has been focused on low-molecular chemicals responsible for HCD. We isolated a low-molecular compound that can induce HCD in tobacco mosaic virus (TMV)-infected tobacco at 30C, a non-permissive temperature for induction of HCD, and identified it as cis-abienol, a diterpene, by physicochemical analyses. Interestingly, the chemical structure of cis-abienol is very similar to that of WAF-1, a diterpene, which was originally isolated as an activator of the tobacco mitogen-activated protein kinase (MAPK) WIPK, cis-abienol was found to induce expression of cell death-related genes and activation of WIPK and SIPK, another tobacco MAPK lb clarify the relationship between cisabienol and these MAPKs, we generated transgenic tobacco plants in which WIPK and SIPK were silenced using RNAi. WIPK/SIPK RNAi plants exhibited inhibited HCD and altered accumulation of jasmonic and salicylic acids in response to infection with TMV or wounding. Treatment of WIPK/SIPK RNAi plants with cis-abienol compromised TMV-induced HCD. These results suggest that cis-abienol is involved in TMV-induced HCD via activation of WIPK and SIPK.
|
