Molecular mechanism of insect metamorphosis revealed by analyzing the brain-expressed genes.

2009 Fiscal Year Final Research Report

• Project/Area Number: 18380040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kanazawa University
• Principal Investigator: IWAMI Masafumi Kanazawa University, 自然システム学系, 教授 (40193768)
• Co-Investigator(Kenkyū-buntansha): MOTO Ken'ichi 独立行政法人理化学研究所, 松本分子昆虫学研究室, 専任研究員 (90333335)
• Co-Investigator(Renkei-kenkyūsha): SAKURAI Sho 金沢大学, 事務局, 副学長 (80143874)
• Project Period (FY): 2006 – 2009
• Keywords: 遺伝子 / 昆虫 / 脳・神経 / 発生・分化 / マイクロアレイ / エクジソン / 前胸腺刺激ホルモン / 神経分泌細胞

Research Abstract

カイコガの変態時における脳の変態制御の中枢としての機能を探るために,発生過程での使用される遺伝子の網羅的解析を試みた。サブトラクションライブラリーから,エクジソン応答遺伝子を10遺伝子単離し,前胸腺刺激ホルモン産生細胞でのみ発現することを示した。マイクロアレイを用いた網羅的解析では,前終齢特異的発現を示す3遺伝子(ADAMTS様タンパク質,チトクロームP450,Kruppel様タンパク質をコード),終齢脳特異的発現を示す遺伝子(クチクラ様タンパク質をコード)を同定した。

Research Products

• [Journal Article] Possible involvement of proteinaceous and non-proteinaceous trehalase inhibitors in the regulation of hemolymph trehalose concentration in Bombyx mori. (2009)
  • Author(s): Tatun, N., Singtripop, T., Osugi, S., Siriluk, N.C., Iwami, M., Sakurai, S.
  • Journal Title: Appl. Entomol. Zool. 44 Pages: 85-94
  • Peer Reviewed
• [Journal Article] Rectal sac distention is induced by 20-hydroxyecdysone in the pupa of Bombyx mori. (2009)
  • Author(s): Suzuki, T., Sakurai, S., Iwami, M.
  • Journal Title: J. Insect Physiol. 55 Pages: 250-254
  • Peer Reviewed
• [Journal Article] Intracellular mobilization of Ca2+ by the insect steroid hormone 20-hydroxyecdysone during programmed cell death in silkworm anterior silk glands. (2009)
  • Author(s): Manaboon, M., Iga, M., Iwami, M., Sakurai, S.
  • Journal Title: J. Insect Physiol. 55 Pages: 123-129
  • Peer Reviewed
• [Journal Article] The genome of a lepidopteran model insect (2008)
  • Author(s): Xia, Q., Xiang, Z., Mita, K., Iwami, M., Shimada, T., Morishita, S.
  • Journal Title: the silkworm Bombyx mori., Insect Biochem. Mol. Biol. 38 Pages: 1036-1045
  • Peer Reviewed
• [Journal Article] Expression of 20-hydroxyecdysone-induced genes in the silkworm brain and their functional analysis in post-embryonic development. (2008)
  • Author(s): Hossain, M., Shimizu, S., Matsuki, M., Imamura, M., Sakurai, S., Iwami, M.
  • Journal Title: Insect Biochem. Mol. Biol. 38 Pages: 1001-1007
  • Peer Reviewed
• [Journal Article] Solubilization of the ecdysone binding protein from anterior silk gland cell membranes of the silkworm (2007)
  • Author(s): Elmogy, M., Iwami, M., Sakurai, S.
  • Journal Title: mori., Zool. Sci. 24 Pages: 971-977
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying sex pheromone production in the silkmoth, Bombyx mori: characterization of the molecular components involved in bombykol biosynthesis. (2007)
  • Author(s): Matsumoto, S., Hull, J.J., Ohnishi, A., Moto, K., Fonagy, A.
  • Journal Title: J. Insect Physiol. 53 Pages: 752-759
  • Peer Reviewed
• [Journal Article] Hormonal regulation of two isoforms of Ets transcription factor E74 gene in programmed cell death in the silkworm anterior silk gland. (2007)
  • Author(s): Sekimoto, T., Iwami, M., Sakurai, S.
  • Journal Title: Insect Mol. Biol. 16 Pages: 581-590
  • Peer Reviewed
• [Journal Article] Nongenomic action of an in sect steroid hormone in steroid-induced programmed cell death. (2007)
  • Author(s): Iga, M., Iwami, M., Sakurai, S.
  • Journal Title: Mol. Cell. Endocr. 263 Pages: 18-28
  • Peer Reviewed
• [Journal Article] A rapid increase in cAMP in response to 20-hydroxyecdysone in the anterior silk glands of the silkworm (2006)
  • Author(s): Elmogy, M., Terashima, J., Iga, M., Iwami, M., Sakurai, S
  • Journal Title: Bombyx mori., Zool. Sci. 23 Pages: 715-719
  • Peer Reviewed
• [Journal Article] EcR expression in the prothoracicotropic hormone-producing neurosecretory cells of the Bombyx mori brain: an indication of the master cells of insect metamorphosis. (2006)
  • Author(s): Hossain, M., Shimizu, S., Fujiwara, H., Sakurai, S., Iwami, M.
  • Journal Title: FEBS J. 273 Pages: 3861-3868
  • Peer Reviewed
• [Journal Article] Developmental profile of annexin IX and its possible role in programmed cell death of the Bombyx mori anterior silk gland. (2006)
  • Author(s): Kaneko, Y., Takaki, K., Iwami, M., Sakurai, S.
  • Journal Title: Zool. Sci. 23 Pages: 533-542
  • Peer Reviewed
• [Journal Article] Physiological requirements for 20-hydroxyecdysone-induced rectal sac distention in the pupa of Bombyx mori.
  • Author(s): Suzuki, T., Sakurai, S., Iwami, M.
  • Journal Title: J. Insect Physiol. (in press)
  • Peer Reviewed
• [Journal Article] Juvenile hormone delays the initiation of rectal sac distention by disrupting ecdysteroid action in the silkworm, Bombyx mori.
  • Author(s): Suzuki, T., Sakurai, S., Iwami, M.
  • Journal Title: Pesticide Biochem. Physiol. (in press)
  • Peer Reviewed
• [Presentation] カイコガ変態期における糖代謝変化と老廃物蓄積の解析 (2009)
  • Author(s): 鈴木匠, 桜井勝, 岩見雅史
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2009-09-19
• [Presentation] カイコガ蛹における直腸嚢膨張の内分泌的コントロール (2009)
  • Author(s): 鈴木匠, 桜井勝, 岩見雅史
  • Organizer: 日本応用動物昆虫学会第53回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-03-30
• [Presentation] カイコガ前部絹糸腺に存在するエクジソン誘導性予定細胞死抑制因子の特性 (2009)
  • Author(s): 松井洋人, 宮腰茉沙美, 掛井基徳, 桜井勝, 岩見雅史
  • Organizer: 日本応用動物昆虫学会第53回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-03-30
• [Presentation] Expression and functional analysis of the 20-hydroxyecdysone- induced genes in brain of the silkworm Bombyx mori: RNAi analysis of bombeil-1, 3, 5 and-8 genes. (2008)
  • Author(s): Aslam, A.F.M., Hossain, M., Shimizu, S., Sakurai, S., Iwami, M.
  • Organizer: 日本分子生物学会第31回大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-11
• [Presentation] カイコガ前部絹糸腺に存在するエクジソン誘導性予定細胞死抑制因子の特性 (2008)
  • Author(s): 松井洋人, 宮腰茉沙美, 掛井基徳, 桜井勝, 岩見雅史
  • Organizer: 日本分子生物学会第31回大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] 幼若ホルモン類似物による直腸嚢膨張の抑制 (2008)
  • Author(s): 鈴木匠, 桜井勝, 岩見雅史
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学(福岡県)
  • Year and Date: 2008-09-05
• [Presentation] Involvement of intracellular Ca2+ in the nongenomic action of 20-hydroxyecdysone induced programmed cell death. (2008)
  • Author(s): Manaboon, M., Iga, M., Iwami, M., Sakurai, S.
  • Organizer: 17th International Ecdysone Workshop
  • Place of Presentation: Ulm University (Germany)
  • Year and Date: 2008-07-21
• [Presentation] 20-Hydroxyecdysone- (20E-) induced genes expression in the brain and their functional analysis by RNAi during development of the silkworm, Bombyx mori. (2007)
  • Author(s): Hossain, M., Shimizu, S., Matsuki, M., Imamura, M., Sakurai, S., Iwami, M.
  • Organizer: 日本分子生物学会第30回大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2007-12-13
• [Presentation] Spatial distribution of 20-hydroxyecdysone (20E)-responsive genes in the brain of silkworm, Bombyx mori. (2007)
  • Author(s): Roy, A., Shimizu, S., Sakurai, S., Mita, K., Iwami, M.
  • Organizer: 日本分子生物学会第30回大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2007-12-13
• [Presentation] カイコガ(Bombyx mori)幼虫の脳における変態調節機構に関与する遺伝子の網羅的解析 (2007)
  • Author(s): 伊東慎矢, 桜井勝, 野田博明, 三田和英, 岩見雅史
  • Organizer: 日本分子生物学会第30回大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2007-12-13
• [Presentation] カイコガ前部絹糸腺細胞死におけるカルモジュリン拮抗薬による核凝縮の阻害 (2007)
  • Author(s): マナブーンマナポーン, 伊賀正年, 岩見雅史, 桜井勝
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学(青森県)
  • Year and Date: 2007-09-22
• [Presentation] Expression and functional analysis of the 20-hydroxyecdysone- (20E-) induced brain genes during development of the silkworm Bombyx mori. (2006)
  • Author(s): Hossain, M., Shimizu, S., Matsuki, M., Imamura, M., Sakurai, S., Iwami, M.
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2006-12-08
• [Presentation] カイコガ幼虫脳において網羅的解析により同定したエクジソン応答遺伝子の発現および機能解析 (2006)
  • Author(s): 清水咲子, Hossain, M., 関本隆志, 桜井勝, 三田和英, 岩見雅史
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2006-12-08
• [Presentation] カイコガにおける昆虫インスリン様ホルモンbombyxinのシグナル伝達機構 (2006)
  • Author(s): 鈴木匠, 伊賀正年, 善岡克次, 桜井勝, 岩見雅史
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2006-12-06
• [Presentation] ボンビキシンによるマルピーギ管でのMAPキナーゼシグナルの活性化 (2006)
  • Author(s): 鈴木匠, 伊賀正年, 善岡克次, 岩見雅史
  • Organizer: 日本動物学会第77回大会
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2006-09-23
• [Presentation] 20E誘導性予定細胞死シグナルカスケードにおけるCa2+の関与 (2006)
  • Author(s): 伊賀正年, 岩見雅史, 桜井勝
  • Organizer: 日本動物学会第77回大会
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2006-09-22
• [Presentation] Genomic and nongenomic actions of 20E in programmed cell death of Bombyx anterior silk gland. (2006)
  • Author(s): Iga, M., Sekimoto, T., Elmogy, M., Iwami, M., Sakurai, S.
  • Organizer: 7th International Workshop on Molecular Biology and Genetics of the Lepidoptera
  • Place of Presentation: Orthodox Academy of Crete (Greece)
  • Year and Date: 2006-08-24
• [Presentation] Interaction of genomic and nongenomic actions of 20-hydroxyecdysone in 20E-dependent development events. (2006)
  • Author(s): Iga, M., Sekimoto, T., Elmogy, M., Iwami, M., Sakurai, S.
  • Organizer: 16th International Ecdysone Workshop
  • Place of Presentation: Ghent University (Belgium)
  • Year and Date: 2006-07-12
• [Presentation] Nongenomic action of 20-hydroxyecdysone in programmed cell death of Bombyx anterior silk gland. (2006)
  • Author(s): Iga, M., Iwami, M., Sakurai, S.
  • Organizer: 16th International Ecdysone Workshop
  • Place of Presentation: Ghent University (Belgium)
  • Year and Date: 2006-07-11
• [Presentation] The prothoracicotropic hormone-producing cells (PTPCs) are the master cells of insect metamorphosis: Exclusive expression of ecdysone receptor genes at the PTPCs in silkworm larval brain. (2006)
  • Author(s): Hossain, M., Shimizu, S., Sakurai, S., Iwami, M.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2006-06-19
• [Presentation] Comprehensive analysis of gene expression induced by 20-hydroxyecdysone (20E) in the silkworm brain: Analysis with microarray. (2006)
  • Author(s): Shimizu, S., Hossain, M., Kaneko, Y., Sakurai, S., Noda, H., Mita, K., Iwami, M.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2006-06-19
• [Book] Ecdysone: Structures and Functions (2009)
  • Author(s): Iga, M., Sakurai, S.
  • Total Pages: 411-423
  • Publisher: Springer
• [Book] ホルモンハンドブック新訂eBook版 (2007)
  • Author(s): 岩見雅史
  • Total Pages: 1787-1799, 1806-1827
  • Publisher: 南江堂
• [Remarks]
  • URL: http://bombyxin.umin.jp