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2008 Fiscal Year Final Research Report

Molecular level study for acetic acid resistance mechanism of acetic acid bacteria

Research Project

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Project/Area Number 18380059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionYamaguchi University

Principal Investigator

SUSHITA Kazunobu  Yamaguchi University, 農学部, 教授 (50107736)

Project Period (FY) 2006 – 2008
Keywords酢酸菌 / 酢酸耐性能 / 好気呼吸鎖 / エネルギー生成能 / 菌膜多糖
Research Abstract

酢酸菌の「酢酸発酵」には, 酢酸を生成するエタノール酸化能に加えて, 生成された高濃度酢酸に対する耐性能が必要である.本研究では, この酢酸耐性機構を, 酸化呼吸鎖や酢酸排出系と関連させて, 解析した.結果, 酢酸菌の属間および種間で様々に異なる能力を示す酢酸耐性能は, 共通して, そのエタノール酸化反応, 特にその中心となるアルコール脱水素酵素, によるエネルギー生成能と密接に関係していること, 細胞膜に存在する酢酸排出系に加えて, 細胞膜リン脂質および細胞表面に存在するヘテロ多糖が重要な役割を果たすことが明らかにした.

  • Research Products

    (29 results)

All 2009 2008 2007 2006 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (13 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A tightly bound quinone functions in ubiquinone reaction sites of quinoprotein alcohol dehydrogenase of acetic acid bacteria, Gluconobacter suboxydans2008

    • Author(s)
      K. Matsushita, Y. Kobayashi, M. Mizuguchi, H. Toyama, O. Adachi, K. Sakamoto, H. Miyoshi
    • Journal Title

      Biosci Biotechnol Biochem 72(10)

      Pages: 2723-2731

    • Peer Reviewed
  • [Journal Article] 耐熱性発酵微生物の発見とその展開2008

    • Author(s)
      松下一信, 赤田倫治, 山田守
    • Journal Title

      化学と生物 46(7)

      Pages: 472-477

    • Peer Reviewed
  • [Journal Article] Molecular taxonomy of Acetobacter syzygii SKU19 and Characterization of its acetic acid adapted strains2008

    • Author(s)
      W. Sintuprapa, G. Theeragool, W. Yongmanitchai, P. Srifah-Huehne, K. Matsushita
    • Journal Title

      Kasetsart J.(Nat. Sci.) 42

      Pages: 701-714

    • Peer Reviewed
  • [Journal Article] タイで分離された耐熱性酢酸菌の機能解析とその利用2008

    • Author(s)
      松下一信
    • Journal Title

      バイオサイエンスとインダストリー 66(3)

      Pages: 130-134

  • [Journal Article] Energy metabolism of unique acetic acid bacterium, Asaia bogorensis, which lacks ethanol oxidation activity2008

    • Author(s)
      Y. Ano, H. Toyama, O. Adachi, K. Matsushita
    • Journal Title

      Biosci. Biotechnol. Biochem 72(4)

      Pages: 989-997

    • Peer Reviewed
  • [Journal Article] 新規な微生物資源としての耐熱性酢酸菌の分離・分類・保存および機能解析2007

    • Author(s)
      松下一信
    • Journal Title

      Institute For Fermentation, Osaka, Research Communications 21

      Pages: 19-29

    • Peer Reviewed
  • [Journal Article] The highly tolerant acetic acid bacterium Gluconacetobacter europaeus adapts to the presence of acetic acid by changes in lipid composition, morphological properties and PQQ-dependent ADH expression2007

    • Author(s)
      Trcek J, Jernejc K, Matsushita K.
    • Journal Title

      Extremophiles 11(4)

      Pages: 627-635

    • Peer Reviewed
  • [Journal Article] Conversion of capsular polysaccharide, involved in pellicleformation, to extracellular polysaccharide bygalE deletion in Acetobacter tropicalis2006

    • Author(s)
      A. Deeraksa, S. Moonmangmee, H. Toyama, O. Adachi and K. Matsushita
    • Journal Title

      Biosci. Biotechnol. Biochem 70

      Pages: 2536-2539

    • Peer Reviewed
  • [Journal Article] Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria2006

    • Author(s)
      J. Trcek, H. Toyama, J. Czuba, A. Misiewicz, and K. Matsushita
    • Journal Title

      Appl. Microbiol. Biotechnol 70(3)

      Pages: 366-373

    • Peer Reviewed
  • [Journal Article] Biochemical and spectroscopic properties of cyanide-insensitive quinol oxidase from Gluconobacter oxydans

    • Author(s)
      T. Mogi, Y. Ano, T. Nakatsuka, H. Toyama, A. Mouri, H. Miyoshi, C. T. Migita, H. Ui, K. Shiomi, S. Omura, K. Kita, K. Matsushita
    • Journal Title

      J. Biochem (in press)

    • Peer Reviewed
  • [Presentation] Characterization in some thermosensitive mutants obtained from thermotolerant Acetobacter tropicalis SKU11002009

    • Author(s)
      W. Soemphol, A. Deeraksa, H. Toyama, O. Adachi, K. Matsushita
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡(講演要旨集)
    • Year and Date
      2009-03-29
  • [Presentation] 高温適応酢酸発酵株の育種とその機能解析2009

    • Author(s)
      秦野智行、今田智子、外山博英, 薬師寿治, 足立収生、松下一信
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡(講演要旨集)
    • Year and Date
      2009-03-28
  • [Presentation] Purification and characterization of alcohol dehydrogenases from thermotolerant Acetobacter pasteurianus strains2008

    • Author(s)
      W. Kanchanarach, O. Adachi, H. Toyama, K. Matsushita
    • Organizer
      2^<nd> International Conference on Acetic Acid Bacteria (2^<nd> ICAAB)
    • Place of Presentation
      held in Nogoya, Japan
    • Year and Date
      20081111-14
  • [Presentation] Respiratory chain related to acetic acid fermentation in Acetobacter pasteurianus IFO 32832008

    • Author(s)
      Y. Matsumura, T. Yakushi, O. Adachi, K. Matsushita
    • Organizer
      2^<nd> International Conference on Acetic Acid Bacteria (2^<nd> ICAAB)
    • Place of Presentation
      held in Nogoya, Japan
    • Year and Date
      20081111-14
  • [Presentation] High Temperature-Acetic Acid Fermentation in Thermotolerant Acetobacter pasteurianus SKU 11082008

    • Author(s)
      T. Hatano, T. Imada, O. Adachi, H. Toyama, K. Matsushita
    • Organizer
      2^<nd> International Conference on Acetic Acid Bacteria (2^<nd> ICAAB)
    • Place of Presentation
      held in Nogoya, Japan
    • Year and Date
      20081111-14
  • [Presentation] Analysis of structure and function of membrane-bound alcohol dehydrogenase from acetic acid bacteria2008

    • Author(s)
      M. Goto, I. Miyahara, K. Hirotsu, Y. Kobayashi, T. Nakatsuka, H. Toyama, O. Adachi, K. Matsushita
    • Organizer
      2^<nd> International Conference on Acetic Acid Bacteria (2^<nd> ICAAB)
    • Place of Presentation
      held in Nogoya, Japan
    • Year and Date
      20081111-14
  • [Presentation] Expression of Quinoprotein Alcohol Dehydrogenase (PQQADH) and Adaptive Responses to High Concentration of Ethanol and Acetic Acid in Thermotolerant Acetic Acid Bacteria2008

    • Author(s)
      G. Theeragool, U. Masud, W. Sintuprapa, H. Toyama, O. Adachi, K. Matsushita
    • Organizer
      2^<nd> International Conference on Acetic Acid Bacteria (2^<nd> ICAAB)
    • Place of Presentation
      held in Nogoya, Japan
    • Year and Date
      20081111-14
  • [Presentation] Isolation and characterization of genes involved in thermotolerance in thermotolerant Acetobacter2008

    • Author(s)
      W. Soemphol, A. Deeraksa, H. Toyama, O. Adachi, K. Matsushita
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      福岡(講演要旨集)
    • Year and Date
      2008-03-28
  • [Presentation] Molecular characterization of quinoprotein alcohol dehydrogenase (PQQADH) and NAD-dependent alcohol dehydrogenases (NAD-ADHs) in thermotolerant acetic acid bacteria2007

    • Author(s)
      G. Theeragool, P. Chinnawirotpisan, W. Sintuprapa, U. Masud, N. Lotong, W. Kanchanarach, H. Toyama, O. Adachi, K. Matsushita
    • Organizer
      The 6^<th> JSPS-NRCT Joint Seminar on Development of Thermotolerant Microbial Resources and Their Applications
    • Place of Presentation
      Walailak, Thailand
    • Year and Date
      20071018-20
  • [Presentation] Production mechanism of pellicle poly- saccharide in thermotolerant acetic acid bacteria2007

    • Author(s)
      S. Moonmangmee, A. Deeraksa, S. Tantratian, H. Toyama, O. Adachi, G. Theeragool, N. Lotong, K. Matsushita
    • Organizer
      The 6^<th> JSPS-NRCT Joint Seminar on Development of Thermotolerant Microbial Resources and Their Applications
    • Place of Presentation
      Walailak, Thailand
    • Year and Date
      20071018-20
  • [Presentation] 酢酸菌アルコール脱水素酵素の立体構造2007

    • Author(s)
      後藤勝, 宮原郁子, 広津建, 小林由宜, 中柄朋子, 外山博英, 足立收生, 松下一信
    • Organizer
      日本生体エネルギー研究会第33回討論会
    • Place of Presentation
      山口大学(講演要旨集)
    • Year and Date
      2007-11-16
  • [Presentation] Acetobacter属酢酸菌のctaAのクローニングとその機能解析2006

    • Author(s)
      加山知穂、小池雅文、滝川雄介、福島浩太、阿野嘉孝、外山博英、松下一信
    • Organizer
      日本生体エネルギー研究会第32回討論会
    • Place of Presentation
      東工大(講演要旨集)
    • Year and Date
      2006-12-14
  • [Presentation] 酢酸菌アルコール脱水素酵素の結合型キノンの機能2006

    • Author(s)
      中柄朋子、小林由宜、外山博英、足立収生、松下一信
    • Organizer
      日本生体エネルギー研究会第32回討論会
    • Place of Presentation
      東工大(講演要旨集)
    • Year and Date
      2006-12-14
  • [Book] 酵素ハンドブック第3版(編集 : 八木達彦, 福井俊郎, 一島英治, 鏡山博行, 虎谷哲夫)2008

    • Author(s)
      松下一信他203名共同執筆
    • Publisher
      朝倉書店
  • [Book] 第1章特性 : 第3節細胞外多糖 : 酢酸菌が生成する菌膜へテロ多糖2008

    • Author(s)
      松下一信
    • Total Pages
      54-64
    • Publisher
      バイオフィルムの基礎と制御(株)エヌ・ティー・エス第1編基礎編
  • [Book] In : Modern Multidisciplinary Applied Microbiology, A. Mendez-Vilas (ed), Wiley- VCH Verlag GmbH & Co. KgaA, Weinheim2006

    • Author(s)
      J. Trcek, H. Toyama, J. Czuba, A. Misiewicz, and K. Matsushita
    • Total Pages
      674-678
    • Publisher
      Towards understanding the acetic acid resistance in Gluconacetobacter europaeus
  • [Remarks] 研究室

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~oubi

  • [Patent(Industrial Property Rights)] 高温酢酸発酵酢酸菌2008

    • Inventor(s)
      松下一信、秦野智行、薬師寿治、足立收生
    • Industrial Property Rights Holder
      山口大学
    • Industrial Property Number
      特願2008-287703
    • Filing Date
      2008-11-10
  • [Patent(Industrial Property Rights)] 酢酸菌Acetobacter pasteurianus IFO3191が産生するヘテロ多糖からなるメラニン生産抑制剤2007

    • Inventor(s)
      大岡朗、松下一信、外山博英、アーパポーン・デラクサ、ソンポーン・ムーマンミー、足立収生
    • Industrial Property Rights Holder
      宇部興産/山口大学
    • Industrial Property Number
      特願2007-96621
    • Filing Date
      2007-04-21

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Published: 2010-06-10   Modified: 2016-04-21  

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