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2007 Fiscal Year Final Research Report Summary

ANALYSIS OF PRIMARY METABOLITE MANAGEMENT ON THE INDUCTION OF SECONDARY METABOLISM IN PL ANTS

Research Project

Project/Area Number 18380066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

NISHIOKA Takaaki  Kyoto University, GRADUATE SCHOOL OF AGRICULTURE, PROFESSOR. (80026559)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Atsushi  KYOTO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, ASSISTANT PROFESSOR (80281103)
Project Period (FY) 2006 – 2007
Keywordsmetabolic flux analysis / metabolic control analysis / Monte Carlo simulations / phenvlpropanoid pathway / elicitor / potato / kinetic network modelling / pulse-feeding experiment
Research Abstract

In the present study we have developed a method that allows dynamic flux analysis without a priori kinetic knowledge in the secondary metabolism of plants. This method was developed and validated using the pulse-feeding experimental data, in which incorporation of exogenously applied L-phenylalanine-d5 into seven phenylpropanoid metabolites in potato tubers was determined. After identification of the topology of the metabolic network of these biosynthetic pathways, the system was described by dynamic mass balances in combination with power-law kinetics. As a next step, the exponents of the power-law kinetics were identified and then kept at fixed values during further analysis. The model was tested for statistical reliability using Monte Carlo simulations. Most fluxes could be identified with high accuracy. The two test cases, control and after elicitation, were clearly distinguished, and with elicitation fluxes to N-p-coumaroyloctopamine (pCO) and N-p-coumaroyltyramine (pCT) increased significantly, whereas those for chlorogenic acid (CGA) and p-coumaroylshikimate decreased significantly. According to the model, increases in the first two fluxes were caused by induction/derepression mechanisms. The decreases in the latter two fluxes were caused by decreased concentrations of their substrates, which in turn were caused by increased activity of the pCO- and pCT-producing enzymes. Flux-control analysis showed that, in most cases, flux control was changed after application of elicitor. Thus the results revealed potential targets for improving actions against tissue wounding and pathogen attack.

  • Research Products

    (26 results)

All 2008 2007 2006

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (22 results) Book (1 results)

  • [Journal Article] Metabolomics of microorganisms2008

    • Author(s)
      Nishioka, T
    • Journal Title

      in "Agriculture in the 21" Century", Edited by Ueda, M., Kyoto University Press(Kyoto) 6

      Pages: 207-248

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Estimation of metabolic fluxes, expression levels and metabolite dynamics of a secondary metabolic pathway in potato using labelpulse feeding experiments combined with kinetic network modeling and simulation2007

    • Author(s)
      Heinzle, E,
    • Journal Title

      Plant Journal 50

      Pages: 176-189

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Estimation of metabolic fluxes, expression levels and metabolite dynamics of a secondary metabolic pathway in potato using label Pulse-feeding experiments combined with kinetic network modelling and simulation2007

    • Author(s)
      Heinzle, E
    • Journal Title

      Plant Journal 50

      Pages: 176-187

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] イネの病害応答おけるセロトニンの役割2008

    • Author(s)
      石原亨
    • Organizer
      日本農薬学 会第33回大会
    • Place of Presentation
      近畿大学,奈良市
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] オオムギの防御物質ホルダチン類の細胞内局在と合成酵素活性の解析2008

    • Author(s)
      石塚晶啓
    • Organizer
      日本農薬学会第33回大会
    • Place of Presentation
      近畿大学,奈良市
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional role of serotonin in defense responses of rice plants2008

    • Author(s)
      Ishihara, A
    • Organizer
      The 33" Annual Meeting of Pesticide Science Society of Japan
    • Place of Presentation
      Nara
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Subcellular compartmentation and biosynthetic enzyme activity for the barley defense substances, hordatines2008

    • Author(s)
      Ishizuka, M
    • Organizer
      The 33rd Annual Meeting of Pesticide Science Society of Japan
    • Place of Presentation
      Nara
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] イネにおいて病原菌の感染によって誘導されるトリプトファン代謝2007

    • Author(s)
      石原亨
    • Organizer
      日本農薬学会第32回大会
    • Place of Presentation
      東京農工大学,府中市
    • Year and Date
      2007-04-03
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Tryptophan metabolism induced by pathogenic infection in rice plants2007

    • Author(s)
      Ishihara, A
    • Organizer
      The 32rd Annual Meeting of Pesticide Science Society of Japan
    • Place of Presentation
      Fuchu
    • Year and Date
      2007-04-03
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 病原菌の感染によって誘導されるトリプトファン由来の二次代謝2007

    • Author(s)
      石原亨
    • Organizer
      日本農芸化学会2006年度大会
    • Place of Presentation
      東京農業大学,東京
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Secondary metabolism derived from tryptophan induced by pathogenic infection2007

    • Author(s)
      Ishihara, A
    • Organizer
      Annual Meeting of Japan Society for Bioscience,. Biotechnology, and Agrochemistry 2006
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Mass Spectral Database for Metabolome Analysis2006

    • Author(s)
      Horai, H
    • Organizer
      Genome Informatics 2006
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      20061218-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Mass spectral database for metabolome analysis2006

    • Author(s)
      Horai, H
    • Organizer
      Genome Informatics 2006
    • Place of Presentation
      Yokohama
    • Year and Date
      20061218-20
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] メタボローム解析-CE-MSの応用と展望-メタボローム・マススペクトルDB-2006

    • Author(s)
      西岡孝明
    • Organizer
      第31回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋国際会議場,名古屋市
    • Year and Date
      20060928-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Metabolomics -Applications of CE-MS -DB of metabolome and mass spectra2006

    • Author(s)
      Nishioka, T
    • Organizer
      The 31st Annual Meeting of Medical Mass Spectrometry Society of Japan
    • Place of Presentation
      Nagoya
    • Year and Date
      20060928-29
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] CE-MSを用いたハイスループットバイオ分析2006

    • Author(s)
      西岡孝明
    • Organizer
      日本分析化学会第55回年会
    • Place of Presentation
      大阪大学理学部,豊中市
    • Year and Date
      20060920-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] High-throughput metabolomics using CE-MS2006

    • Author(s)
      Nishioka, T
    • Organizer
      The 55th Annual Meeting of Analytical Chemistry Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20060920-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Metabolomics by using CE-MS2006

    • Author(s)
      Nishioka, T
    • Organizer
      BIOSYSTEMS ENGINEERING
    • Place of Presentation
      ベルビユウホテル,ブラウンバルト,スイス
    • Year and Date
      20060910-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Metabolomics using CE-MS2006

    • Author(s)
      Nishioka, T
    • Organizer
      Biosystems Engineering
    • Place of Presentation
      Braunwald
    • Year and Date
      20060910-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Metabolomics with CE-MS analysis is a tool of post-genome science2006

    • Author(s)
      Nishioka, T.
    • Organizer
      国際分析科学会議(ICAS-2006)
    • Place of Presentation
      ロシア学士院会館,モスクワ,ロシア
    • Year and Date
      20060625-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Metabolomics with CE-MS analysis is a tool of post-genome sciences2006

    • Author(s)
      Nishioka, T
    • Organizer
      International Conference of Analytical Sciences 2006
    • Place of Presentation
      Moscow
    • Year and Date
      20060625-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Dynamic modeling of Escherichia coli central carbon metabolism basedon multi-omics data2006

    • Author(s)
      Ishii, N
    • Organizer
      第20回国際生化学・分子生物学会議
    • Place of Presentation
      京都国際会議場,京都
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dynamic modeling of Escherichiacoli central carbon metabolism based on multi-omics data2006

    • Author(s)
      Ishii, N
    • Organizer
      The 20th International Conference of Biochemistry and Molecular Biology
    • Place of Presentation
      Kyoto
    • Year and Date
      20060618-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 細胞内全代謝物質の同定,定量に向けての戦略2006

    • Author(s)
      西岡孝明
    • Organizer
      第54回質量分析総合討論会
    • Place of Presentation
      千里ライフサイエンスセンター,豊中市
    • Year and Date
      20060517-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Strategy toward identification and quantification of the whole metabolites in cells2006

    • Author(s)
      Nishioka, T
    • Organizer
      The 54th Symposium on Mass Spectro-metry Society of Japan
    • Place of Presentation
      Toyonaka
    • Year and Date
      20060517-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 21世紀の農学(第6巻)2008

    • Author(s)
      西岡 孝明
    • Total Pages
      207-248
    • Publisher
      京都大学出版会
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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