2007 Fiscal Year Final Research Report Summary
Research on the regulation of gibberellin receptors
| Project/Area Number |
18380070
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAJIMA Masatoshi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant (50237278)
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| Project Period (FY) |
2006 – 2007
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| Keywords | gibberellin / receptor / signal transduction / nuclear receptor |
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| Research Abstract |
Arabidopsis carries three receptor genes for the phytohormone gibberellin (GA), AtGID1a, AtGID1b, and AtGID1c. The expression of each gene in the rice gid1-1 mutant for GA-receptor reverts its severely dwarfed phenotype and GA-insensitivity to a normal level, even though each loss-of-function mutant shows no clear phenotype in Arabidopsis. We report the functional redundancy and specificity of each AtGID1 by analyzing the multiple mutants for loss-of-function mutation. Seeds of double KO mutants, atgid1a+atgid1b, atgid1a+atgid1c, and atgid1b+atgid1c, normally germinated. The double KO mutant, atgid1a+atgid1c showed a dwarf phenotype, while other double mutants had a normal height comparable to the wild type. Stamens of the double KO mutant, atgid1a+atgid1b are significantly much shorter than those of wild type, and this leads to low fertility A severe disarrangement of the pattern on its seed surface was also observed. The triple KO mutant, atgid1a+atgid1b+atgid1c did not germinate voluntarily, but could only start to grow when the seed coat was peeled off after soaking. Seedlings of the triple KO mutants were severe dwarfs several millimeters high after growing for one month. Moreover the triple KO seedlings completely lost their ability to respond to exogenously applied GA. These results show that all AtGID1s function as GA receptors in Arabidopsis, and in part have specific role(s) for growth and development.
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[Journal Article] Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal2007
Author(s)
S. luchi, H. Suzuki, Y.-C. Kim, A. luchi, T. Kuromori, M. Ueguchi-Tanaka, T. Asami, I. Yamaguchi, M. Matsuoka, M. Kobayashi, M. Nakaiima.
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Journal Title
Plant J. 50
Pages: 958-966
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of Arabidopsis gibberellin receptors2006
Author(s)
M. Nakaiima, A. Shimada, Y. Takashi, Y.-C. Kim, S.-H. Park, M. Ueguchi-Tanaka, H. Suzuki, E. Katoh, S. luchi, M. Kobayashi, T. Maeda, M. Matsuoka, I. Yamaguchi.
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Journal Title
Plant J. 46
Pages: 880-889
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Characterization of knockout mutants for gibberellin receptor AtGID1 s in Arabidopsis thaliana2007
Author(s)
S. luchi, H. Suzuki, Y.-C. Kim, A. luchi, T. Kuromori, M. Ueguchi-Tanaka, T. Asami, I. Yamaguchi, M. Matsuoka, M. Nakajima, M. Kobayashi
Organizer
19th Internatinal Conference on Plant Growth Substances
Place of Presentation
Puerto Vallarta, Mexico
Year and Date
20070700
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Characterization of Arabidopsis gibberellin receptors2007
Author(s)
M. Nakajima, A. Shimada, Y. Takashi, Y.-C. Kim, S.-H. Park, M. Ueguchi-Tanaka, H. Suzuki, E. Katoh, S. luchi, M. Kobayashi, T. Maeda, M. Matsuoka, I. Yamaguchi
Organizer
19th Internatinal Conference on Plant Growth Substances
Place of Presentation
Puerto Vallarta, Mexico
Year and Date
20070700
Description
「研究成果報告書概要(欧文)」より
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