2007 Fiscal Year Final Research Report Summary
Study of the mucosal immunomoduLation by intestinal commensal bacteria
| Project/Area Number |
18380084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nihon University |
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Principal Investigator |
KAMINOGAWA Shuichi Nihon University, College of Bioresource Sciences, Professor (50011945)
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| Co-Investigator(Kenkyū-buntansha) |
HOSONO Akira Nihon University, College of Bioresource Sciences, Associate Professor (70328706)
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| Project Period (FY) |
2006 – 2007
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| Keywords | allergy / intestinal microbacteria / immunology / gene |
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| Research Abstract |
Intestinal commensal bacteria play important roles in the regulation of intestinal immune responses such as anti-allergic reaction and anti-infection. It has been shown that intestinal commensal bacteria greatly affect the development of gut-associated lymphoid tissues and mucosal immune responses, such as IgA production, in the gut. Given the large numbers of intestinal microbiota, particularly in the large intestine, it is believed that immunocytes in the large intestine are modulated by microbacteria. While IgA production by immunocytes in the small intestine has been previously studied, IgA production in the large intestine is, however, poorly understood. In this study, we show that IgA production by lymphocytes is induced by microbacteria present in the large intestine.
Lamina propria lymphocytes from the large intestine (L-LP) were isolated from germ-free (GF) and conventional (CV) mice. Flow cytometric analysis of L-LP lymphocytes was used to assess the frequency of IgM+B220+ cel
… More
ls, IgA+B220+ cells, and IgA+Syndecan-l+B220- cells. In addition, to determine whether stimulation by commensal bacteria influences differentiation of IgM+ cells into IgA-plasma cells, IgM+ cells separated from L-LP lymphocytes of GF mice were co-cultured with Bacteroides acidofaciens isolated from intestinal commensal bacteria of CV mice.
The frequency of IgA+Syndecan-1+B220- cells and IgA+B220+ cells in L-LP of GF were all lower than that of CV mice, but there was no difference in the frequency of IgM+13220+ cells in L-LP between GF and CV mice. IgA production by IgM+ cells stimulated by B. acidofaciens was higher than in those without bacterial stimulation.
These results indicate that direct stimulation by B. acidofaciens induces differentiation of IgM+ cells into IgA-producing plasma cells from the large intestine. These also suggest that IgA production in the large intestine might be induced by intestinal commensal bacteria, thus intestinal microbacteria promote the class switching from IgM to IgA in the large intestine. Less
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[Journal Article] Identification of marker genes for intestinal immunomodulating effect of afructooligosaccharide by DNA microarray analysis2007
Author(s)
Fukasawa T, Murashima K, Matsumoto I, Hosono A, OharaH, Nojiri C, Koga J, Kubota H, Kanegae M, Kaminogawa S, Abe K, Kono T.
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Journal Title
J Agric Food Chem 55
Pages: 3174-3179
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Food antigen causes TH2-dependent enteropathy followed by tissue repairin T-cell receptor transgenic mice2006
Author(s)
Nakajima-Adachi H, Ebihara A, Kikuchi A, Ishida T, Sasaki K, Hirano K, Watanabe H, Asai K, Takahashi Y, Kanamori Y, Shimojo N, Matsuda H, Kohno Y, Hachimura S, Kaminogawa S
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Journal Title
J. Allergy Clin.Immunol 117
Pages: 1125-1132
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Increase in terminal fragments of 16S rRNA genes derived from Bacteroidetes after administration of short-chain fructooligosaccharides2006
Author(s)
Nakanishi Y, Murashima K, Ohara H, Suzuki T, Hayashi H, Sakamoto M, Fukasawa T, Kubota H, Hosono A, Kono T, Kaminogawa S, Benno Y
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Journal Title
Appl.Environ.Microbiol 72
Pages: 6271-6276
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Presentation] Intestinal CD3-IL-2R^+cells respond to poly I:C stimuli and influenza virusinfection2007
Author(s)
Umeda Y, Kuraoka M, Takahashi Y, Yamada K, Tsuji N, Kouro T, Totsuka M, Takatsu K, Kaminogawa S, Sato R, Hachimura S
Organizer
第37回日本免疫学会総会・学術集会
Place of Presentation
東京
Year and Date
20071120-22
Description
「研究成果報告書概要(和文)」より
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[Presentation] CD3-IL-2R^+ Peyer's patch cells respond to TLR Stimuli,secrete IL-5,and induce IgA production2007
Author(s)
Umeda Y, Kuraoka M, Yamada K, Kouro T, Takahashi Y, Tsuji NM, Totsuka M, Takatsu K, Kaminogawa S, Sato R, Hachimura S
Organizer
13th International Congress of Mucosal Immunology(ICMI 2007)
Place of Presentation
Tokyo,Japan
Year and Date
20070709-12
Description
「研究成果報告書概要(和文)」より
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[Presentation] CD3IL-2R^+ Peyer's patch cells respond to poly I:C stimuli and secrete IL-52006
Author(s)
Umeda Y, Akema Y, Kuraoka M, Yamada K, Hashiguchi M, Ise W, Kouro T, Takahashi Y, Totsuka M, Takatsu K, Kaminogawa S, Sato R, Hachimura S
Organizer
第36回日本免疫学会総会・学術集会
Place of Presentation
大阪
Year and Date
20061211-13
Description
「研究成果報告書概要(和文)」より
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