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2008 Fiscal Year Final Research Report

Studies on breeding technology using natural clonal and polyploid fish as genetic resources

Research Project

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Project/Area Number 18380108
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

ARAI Katsutoshi  Hokkaido University, 大学院・水産科学研究院, 教授 (00137902)

Co-Investigator(Kenkyū-buntansha) ABE Shuichi  北海道大学, 大学院・水産科学研究院, 教授 (80125278)
YAMAHA Etsuro  北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376)
MURAKAMI Masaru  麻布大学, 獣医学部, 教授 (80271360)
Project Period (FY) 2006 – 2008
Keywordsクローン / 倍数体 / 雌性発生 / 雄性発生 / 性転換 / 減数分裂 / 単為発生 / 非還元配偶子
Research Abstract

ドジョウは両性生殖で繁殖するのが普通であるが、通常の減数分裂によらず非還元二倍体の卵を産み、かつ、これらの卵が雌性発生により父親の遺伝的関与なく発生するクローン系統、および偶然の精子核取り込みにより生じる自然三倍体系統が野生集団に見られる。本研究は、このような特殊な生殖の機構と起源を明らかにするとともに、このような変異体を遺伝資源(育種素材)として、交雑・染色体操作と組み合わせることにより育種技術開発を行った。

  • Research Products

    (34 results)

All 2009 2008 2007 2006 0

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (20 results)

  • [Journal Article] ドジョウの倍数性とクローン、それらの特殊な生殖様式2009

    • Author(s)
      荒井克俊
    • Journal Title

      動物遺伝育種 37(1)(印刷中)

    • Peer Reviewed
  • [Journal Article] A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species2009

    • Author(s)
      Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K
    • Journal Title

      Animal Reproduction Science (DOI 10.1016/j.anireprosci.) (in press)

      Pages: 10.1016

    • Peer Reviewed
  • [Journal Article] Chromosome doubling in early spermatogonia produces diploid spermatozoa in a natural clonal fish2009

    • Author(s)
      Yoshikawa H, Morishima K, Fujimoto T, Saito T, Kobayashi T, Yamaha E, Arai K
    • Journal Title

      Biology of Reproduction (DOI10.1095/biolreprod.108.075150) 80

      Pages: 973-979

    • Peer Reviewed
  • [Journal Article] Inter-populational difference in microsatellite-centromere mapdistances in the loach, Misgurnus anguillicaudatus2009

    • Author(s)
      Arias-Rodriguez L, Morishima K, Arai K
    • Journal Title

      Ichthyol Res (DOI 10.1007/s10228-008-0077-7) 56

      Pages: 126-132

    • Peer Reviewed
  • [Journal Article] Disruption of normal meiosis in artificial inter-populational hybrid females of Misgurnus loach2009

    • Author(s)
      Arias-Rodriguez L, Yasui GS, Arai K
    • Journal Title

      Genetica (DOI 10.1007/s10709-008-9299x) 136

      Pages: 49-56

    • Peer Reviewed
  • [Journal Article] Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice2008

    • Author(s)
      Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K
    • Journal Title

      CryoLetters 29(5)

      Pages: 383-390

    • Peer Reviewed
  • [Journal Article] Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female and M.mizolepis male2008

    • Author(s)
      Fujimoto T, Yasui GS, Yoshikawa H, Yamaha E, Arai K
    • Journal Title

      J Appl Ichthyol (DOI 10.1111/j.1439-0426.2008.01131.x) 24

      Pages: 430-437

    • Peer Reviewed
  • [Journal Article] Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus : a review2008

    • Author(s)
      Yoshikawa H, Morishima K, Fujimoto T, Arias-Rodriguez L, Yamaha E, Arai K
    • Journal Title

      J Appl Ichthyol (DOI 10.1111/j.1439-0426.2008.01129.x) 24

      Pages: 410-414

    • Peer Reviewed
  • [Journal Article] Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage2008

    • Author(s)
      Morishima K, Yoshikawa H, Arai K
    • Journal Title

      Heredity (DOI 10.1038/hdy.2008.17) 100

      Pages: 581-586

    • Peer Reviewed
  • [Journal Article] 中国におけるドジョウ倍数体の分布に関する研究2008

    • Author(s)
      李雅娟, 印傑, 王嘉博, 袁旭, 魏傑, 孫効文, 荒井克俊
    • Journal Title

      日本水産学会誌 74(2)

      Pages: 177-182

    • Peer Reviewed
  • [Journal Article] Genetic linkage map of the loach Misgurnus anguillicaudatus2008

    • Author(s)
      Morishima K, Nakayama I, Arai K
    • Journal Title

      Genetica (DOI 10.1007/s10709-007-9165-2) 132

      Pages: 227-241

    • Peer Reviewed
  • [Journal Article] Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei : Cobitidae) inferred from nuclear and mitochondrial DNA analyses2008

    • Author(s)
      Morishima K, Nakamura-Shiokawa Y, Bando E, Li YJ, Boron A, Khan MMR, Arai K
    • Journal Title

      Genetica (DOI 10.1007/s10709-007-9158-1) 132

      Pages: 159-171

    • Peer Reviewed
  • [Journal Article] Evaluation of different doses of UV irradiation to loach eggs for genetic inactivation of the maternal genome2007

    • Author(s)
      Fujimoto T, Sakao S, Yamaha E, Arai K
    • Journal Title

      J Exp Zool(DOI 10.1002/jez.398) 307A

      Pages: 449-462

    • Peer Reviewed
  • [Journal Article] Simultaneous formation of haploid, diploid and triploid eggs in diploid-triploid mosaic loaches2007

    • Author(s)
      Yoshikawa H, Morishima K, Fujimoto T, Yamaha E, Arai K
    • Journal Title

      J Fish Biology (DOI 10.1111/j.1095-8649.2009.01608.x) 71(Supplement B)

      Pages: 250-263

    • Peer Reviewed
  • [Presentation] 外来倍数体ドジョウの起源2009

    • Author(s)
      李雅娟, 荒井克俊
    • Organizer
      平成21(2009)年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス, 東京都
    • Year and Date
      2009-03-28
  • [Presentation] Genetic differentiation inferred from sequences of mitochondrial DNA control region of the loach Misgurnus anguillicaudatus in China2008

    • Author(s)
      Li YJ, Sun XW, Yuan X, Li Y, Arai K
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      20081021-22
  • [Presentation] Molecular cytogenetics of viable and inviable salmonid hybrids2008

    • Author(s)
      Abe S, Fujiwara A, Ito D, Sato K, Yamaguchi M, Zheng L, Kimura S, Yamaha E
    • Organizer
      5^<th> World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2008-10-22
  • [Presentation] Genetic markers, mapping and manipulation for analyses of mechanisms of atypical reproduction in the loach, Misgurnus anguillicaudatus2008

    • Author(s)
      Arai K, Morishima K, Yoshikawa H, Arias-Rodriguez L, Fujimoto T, Yamaha E
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Yokohama
    • Year and Date
      2008-10-22
  • [Presentation] クローンドジョウの起源2008

    • Author(s)
      荒井克俊, 森島輝, 吉川廣幸, 李雅娟
    • Organizer
      日本動物学会北海道支部第54回大会
    • Place of Presentation
      北海道大学学術交流会館, 札幌
    • Year and Date
      2008-08-09
  • [Presentation] Genetic marker, map and manipulation in aquatic species2008

    • Author(s)
      Arai K
    • Organizer
      World Aquaculture 2008
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2008-05-23
  • [Presentation] 雑種と異質三倍体は借り腹生産の不妊化宿主として有効か2008

    • Author(s)
      藤本貴史, Yasui GS, 吉川廣幸, 山羽悦郎, 荒井克俊
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部, 静岡市
    • Year and Date
      2008-03-30
  • [Presentation] ドジョウ集団間におけるマーカー-動原体間地図距離の相違2008

    • Author(s)
      アリアス-ロドリゲスレニン, 森島輝, 荒井克俊
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部, 静岡市
    • Year and Date
      2008-03-28
  • [Presentation] ドジョウ雌とカラドジョウ雄間の雑種・異質三倍体は不妊か?2007

    • Author(s)
      藤本貴史・Yasui GS, 吉川廣幸
    • Organizer
      平成19年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学水産学部, 函館市
    • Year and Date
      2007-09-27
  • [Presentation] クローン四倍体ドジョウは減数分裂により二倍体精子をつくる2007

    • Author(s)
      吉川廣幸, 森島輝, 藤本貴史, 荒井克俊
    • Organizer
      平成19年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学水産学部, 函館市
    • Year and Date
      2007-09-27
  • [Presentation] ストローとドライアイスを用いた冷却速度制御による精子凍結保存の新方法2007

    • Author(s)
      Yasui GS, 藤本貴史, 荒井克俊
    • Organizer
      平成19年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学水産学部, 函館市
    • Year and Date
      2007-09-27
  • [Presentation] Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female and M.mizolepis male2007

    • Author(s)
      Fujimoto T, Yasui GS, Yoshikawa H, Yamaha E, Arai K
    • Organizer
      The 1st International Workshop on the Biology of Fish Sperm
    • Place of Presentation
      Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech
    • Year and Date
      2007-08-29
  • [Presentation] Ploidy manipulation using diploid sperm in the loach, Misgurnus anguillicaudatus : a review2007

    • Author(s)
      Yoshikawa H, Morishima K, Fujimoto T, Arias-Rodriguez L, Yamaha E, Arai K
    • Organizer
      The 1^<st> International Workshop on the Biology of Fish Sperm
    • Place of Presentation
      Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech
    • Year and Date
      2007-08-29
  • [Presentation] 自然クローンドジョウの非還元卵形成とゲノムセット構成の関係2007

    • Author(s)
      森島輝, 荒井克俊
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学、東京
    • Year and Date
      2007-03-28
  • [Presentation] ドジョウの地域集団間雑種第一代に見られた非還元2n卵形成2007

    • Author(s)
      アリアスーロドリゲスレニン, 荒井克俊
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学、東京
    • Year and Date
      2007-03-28
  • [Presentation] ドジョウの地域集団間雑種はごく少量の1nおよび2n精子をつくる2007

    • Author(s)
      アリアスーロドリゲスレニン, 楠田聡, 荒井克俊
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学、東京
    • Year and Date
      2007-03-28
  • [Presentation] 借り腹生産における三倍体の不妊宿主としての有効性2007

    • Author(s)
      藤本貴史, 吉川廣幸, 森島輝, 山羽悦郎, 荒井克俊
    • Organizer
      平成19年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学、東京
    • Year and Date
      2007-03-28
  • [Presentation] A genetic map of the loach Misgurnus anguillicaudatus (Teleostei : Cobitidae)2006

    • Author(s)
      Morishima K, Arias-Rodriguez L, Nakayama I, Arai K
    • Organizer
      9^<th> International Symposium Genetics in Aquaculture
    • Place of Presentation
      Montpellier, France
    • Year and Date
      20060626-30
  • [Presentation] Recent progress in reproductive biology and genetics of Misgurnus loaches2006

    • Author(s)
      Morishima K, Fujimoto T, Yoshikawa H, Arai K
    • Organizer
      3^<rd> International Conference, Loaches of the Genus Cobitis and Related Genera
    • Place of Presentation
      Sibenik, Croatia
    • Year and Date
      2006-09-25
  • [Presentation] Hybridogenesis-like oogenesis in triploid females derived from the clone lineage of Misgurnus loach0

    • Author(s)
      Morishima K, Oshima K, Arai K
    • Organizer
      9^<th> International Symposium Genetics in Aquaculture
    • Place of Presentation
      Montpellier, France
    • Year and Date
      00000628

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Published: 2010-06-10   Modified: 2016-04-21  

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