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2008 Fiscal Year Final Research Report

Basic studies on the prevention and improvement on intestinal bowel disease by immunobiotics and their advanced application

Research Project

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Project/Area Number 18380158
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionTohoku University

Principal Investigator

KITAZAWA Haruki  Tohoku University, 大学院・農学研究科, 准教授 (10204885)

Co-Investigator(Kenkyū-buntansha) SAITO Tadao  東北大学, 大学院・農学研究科, 教授 (00118358)
ASO Hisashi  東北大学, 大学院・農学研究科, 准教授 (50241625)
KAWAI Yasushi  東北大学, 大学院・農学研究科, 助教 (00261496)
Project Period (FY) 2006 – 2008
Keywordsプロバイオティクス / イムノバイオティクス / 乳酸菌 / 炎症性腸疾患 / 抗炎症性 / パタ-ン認識受容体 / ブタ
Research Abstract

本研究は、炎症性腸疾患の予防に有効な、新規「イムノバイオティック食品」の開発基盤を築くことを目的とし、ヒトモデルとして期待されるブタ腸管上皮細胞株(PIE細胞)を用いて、イムノバイオティクスの抗炎症活性について解析した。その結果、パタ-ン認識受容体を介して抗炎症性を発揮するイムノバイオティクスの選抜が可能となり、その機構解明が飛躍的に進むことが大いに期待され、「イムノバイオティック食品」の創製に向けた開発基礎が得られた。

  • Research Products

    (31 results)

All 2009 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (16 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Utilization of the porcine system to study lymphotoxin-β regulation in intestinal lymphoid tissue2009

    • Author(s)
      Shimazu, T., M. Tohno, S. Katoh, T. Shimosato, H. Aso, Y. Kawai, T. Saito, H. Kitazawa
    • Journal Title

      Biochem.Genet 47

      Pages: 126-136

    • Peer Reviewed
  • [Journal Article] ヒトモデルとしてのブタにおけるTLRファミリ-の解析と乳業界における発展的利用性2009

    • Author(s)
      遠野雅徳、齋藤忠夫、北澤春樹
    • Journal Title

      ミルクサイエンス 57

      Pages: 79-85

    • Peer Reviewed
  • [Journal Article] Physiol-Gastrointest2008

    • Author(s)
      Kanaya, T., K. Miyazawa, I. Takakura, W. Itani, K. Watanabe, S. Ohwada, H. Kitazawa, M. T. Rose, H. R. McConochie, H. Okano, T. Yamaguchi, H. Aso. Am. J
    • Journal Title

      Liver Phys 295

      Pages: G273-284

    • Peer Reviewed
  • [Journal Article] Molecular cloning and functional characterization of porcine nucleotide- binding oligomerization domain-1 (NOD1) recognizing minimum agonists2008

    • Author(s)
      Tohno, M., T. Shimazu, H. Aso, A. Uehara, H. Takada, A. Kawasaki, Y. Fujimoto, K. Fukase, T. Saito, H. Kitazawa
    • Journal Title

      meso- diaminopimelic acid and meso-lanthionine. Mol. Immunol 45

      Pages: 1807-1817

    • Peer Reviewed
  • [Journal Article] Immunostimulatory oligo- deoxynucleotide containing TTTCGTTT motif from Lactobacillus rhamnosus GG DNA potentially suppresses OVAspecific IgE production in mice2008

    • Author(s)
      Iliev, I.D., M. Tohno, D. Kurosaki, T. Shimosato, F. He, M. Hosoda, T. Saito, H. Kitazawa
    • Journal Title

      Scand. J. Immunol 67

      Pages: 370-376

    • Peer Reviewed
  • [Journal Article] Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line2008

    • Author(s)
      Moue, M., M. Tohno, T. Shimazu, T. Kido, H. Aso, T. Saito, H. Kitazawa
    • Journal Title

      BBA-Gen. Subjects 1780

      Pages: 134-144

    • Peer Reviewed
  • [Journal Article] Molecular cloning and functional characterization of porcine Nucleotide- binding oligomerization domain-2 (NOD2)2008

    • Author(s)
      Tohno, M., W. Ueda, Y. Azuma, T. Shimazu, S. Katoh, J.M. Wang, H. Aso, H. Takada, Y. Kawai, T. Saito, H. Kitazawa
    • Journal Title

      Mol. Immunol 45

      Pages: 194-203

    • Peer Reviewed
  • [Journal Article] Development of molecular immuno- assay system for probiotics via Toll-like receptors based on food immunology2008

    • Author(s)
      Kitazawa, H., M. Tohno, T. Shimosato, T. Saito
    • Journal Title

      Anim. Sci. J 79

      Pages: 11-21

    • Peer Reviewed
  • [Journal Article] Molecular cloning and functional characterization of porcine MyD88 essential for TLR signaling2007

    • Author(s)
      Tohno, M., T. Shimazu, H. Aso, Y. Kawai, T. Saito, H. Kitazawa
    • Journal Title

      Cell. Mol. Immunol 4

      Pages: 369-376

    • Peer Reviewed
  • [Journal Article] An advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 22007

    • Author(s)
      Tohno, M., T. Shimosato, Y. Kawai, H. Aso, S. Ikegami, N. Taketomo, T. Saito, H.Kitazawa
    • Journal Title

      Anim. Sci. J 78

      Pages: 195-205

    • Peer Reviewed
  • [Journal Article] Molecular cloning of porcine RP105/MD-1 involved in recognition of extracellular phosphopolysaccharides from Lactococcus lactis ssp. Cremoris2007

    • Author(s)
      Tohno, M., T. Shimazu, W. Ueda, D. Anzawa, H. Aso, J. Nishimura, Y. Kawai, Y. Saito, T. Saito, H. Kitazawa
    • Journal Title

      Mol. Immunol 44

      Pages: 2566-2577

    • Peer Reviewed
  • [Journal Article] Toll-like receptor 2 and 9 are expressed and functional in gut-associated lymphoid tissues of presuckling newborn swine2006

    • Author(s)
      Tohno, M., T. Shimosato, M. Mouea, H. Aso, K. Watanabe, Y. Kawai, T. Yamaguchi, T. Saito, H. Kitazawa
    • Journal Title

      Vet. Res 37

      Pages: 791-812

    • Peer Reviewed
  • [Journal Article] 豚腸管におけるToll様受容体の機能発現と食品免疫学への応用2006

    • Author(s)
      北澤春樹、遠野雅徳、下里剛士、齋藤忠夫
    • Journal Title

      獣医畜産新報 59

      Pages: 559-561

    • Peer Reviewed
  • [Presentation] イムノバイオティクスのブタ腸管上皮細胞における抗炎症作用2009

    • Author(s)
      島津朋之、遠野雅徳、下里剛士、麻生久、川井泰、齋藤忠夫、北澤春樹
    • Organizer
      日本畜産学会第110回大会
    • Place of Presentation
      日本大学、藤沢市
    • Year and Date
      20090327-29
  • [Presentation] Streptococcus thermophilus lacZからの強免疫刺激性DNAモチーフの発見2009

    • Author(s)
      下里剛士、遠野雅徳、西村順子、川井泰、齋藤忠夫、北澤春樹
    • Organizer
      日本畜産学会第110回大会
    • Place of Presentation
      日本大学、藤沢市
    • Year and Date
      20090327-29
  • [Presentation] An immuno- evaluation system for anti-inflammatory probiotics using originally established porcine epitheliocyte(PIE) cells2008

    • Author(s)
      Shimazu, T., M. Tohno, M. Moue, H. Aso, T. Saito, H. Kitazawa
    • Organizer
      ADSA-ASAS Joint Meeting
    • Place of Presentation
      Indianapolis, USA
    • Year and Date
      20080707-11
  • [Presentation] Immuno-stimulatory AT oligo- deoxynucleotide from Lactobacillus gasseri requires a specific self- stabilized structure2008

    • Author(s)
      Shimosato, T., M. Tohno, T. Sato, Y. Kawai, T. Saito, H. Kitazawa
    • Organizer
      ADSA-ASAS Joint Meeting
    • Place of Presentation
      Indianapolis, USA
    • Year and Date
      20080707-11
  • [Presentation] ブタ腸管上皮細胞によるプロバイオティクスの新規ヒトモデル腸管抗炎症評価系の構築2008

    • Author(s)
      島津朋之、遠野雅徳、馬上真之、藤江瞳、川井泰、麻生久、齋藤忠夫、北澤春樹
    • Organizer
      日本畜産学会第109回大会
    • Place of Presentation
      水戸市
    • Year and Date
      20080327-29
  • [Presentation] Development of evaluation system for immunobiotics, Multilateral Workshop2008

    • Author(s)
      Kitazawa, H., H. Aso
    • Organizer
      Challenge for the reduction of antimicrobial use in animal production
    • Place of Presentation
      つくば国際会議場、 つくば
    • Year and Date
      2008-11-11
  • [Presentation] ブタ腸管におけるNOD1および2の発現とイムノバイオティクスによる発現増強2007

    • Author(s)
      遠野雅徳、植田 渉、島津朋之、森江恭子、麻生久、川井泰、齋藤忠夫、北澤春樹
    • Organizer
      日本畜産学会第108回大会
    • Place of Presentation
      岡山市
    • Year and Date
      20070926-27
  • [Presentation] Immunoregulatory Factors from Probiotics.(invited)2007

    • Author(s)
      Kitazawa, H., M. Tohno, T. Saito
    • Organizer
      13th International Congress of Mucosal Immunology
    • Place of Presentation
      Shinagawa, Tokyo
    • Year and Date
      20070710-11
  • [Presentation] ブタ腸管におけるイムノバイオティクスのTLR2を介する免疫機能発現2007

    • Author(s)
      遠野雅徳、島津朋之、川井泰、齋藤忠夫、麻生久、北澤春樹
    • Organizer
      日本畜産学会第107回大会
    • Place of Presentation
      相模原市
    • Year and Date
      20070327-29
  • [Presentation] ブタlymphotoxin-β(LT-β)のクロ-ニングと腸管における発現増強2007

    • Author(s)
      島津朋之、遠野雅徳、加藤真一郎、下里剛士、麻生久、 川井泰、 齋藤忠夫、 北澤春樹
    • Organizer
      日本畜産学会第107回大会
    • Place of Presentation
      相模原市
    • Year and Date
      20070327-29
  • [Presentation] Preferential expression of lymphotoxin-β by immunobiotics in GALTs of neonatal swine2007

    • Author(s)
      Shimazu, T., M. Tohno, S. Katoh, T. Shimosato, H. Aso, Y. Kawai, T. Saito, H. Kitazawa
    • Organizer
      13th International Congress of Mucosal Immunology
    • Place of Presentation
      Shinagawa, Tokyo
    • Year and Date
      2007-10-11
  • [Presentation] Extra- cellular phosphopolysaccharides from Lactococcus lactis ssp. cremoris is a novel ligand for porcine RP105/MD-1 strongly expressed in gut-associated lymphoid tissues2007

    • Author(s)
      Tohno, M., T. Shimazu, W. Ueda, D. Anzawa, H. Aso, J. Nishimura, Y. Kawai, Y. Saito, T. Saito, H. Kitazawa
    • Organizer
      13th International Congress of Mucosal Immunology
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-10-11
  • [Presentation] TLRの腸管における機能発現を基礎としたイムノバイオティック評価系の構築2006

    • Author(s)
      遠野雅徳、北澤春樹
    • Organizer
      日本食品免疫学会, 第2回学術大会シンポジウム1
    • Place of Presentation
      昭和女子大学、東京
    • Year and Date
      20061023-24
  • [Presentation] プロバイオティクスの細胞内レセプターNodを介する分子免疫活性評価系の構築2006

    • Author(s)
      四阿祐子、遠野雅徳、下里剛士、加藤真一郎、川井泰、齋藤忠夫、北澤春樹
    • Organizer
      第56回東北畜産学会大会
    • Place of Presentation
      秋田市
    • Year and Date
      20060831-0901
  • [Presentation] Development of a novel immunoassay system for immunobiotics that madulate intestinal immunity through Toll-like receptor 22006

    • Author(s)
      Tohno, M., T. Shimosato, Y. Kawai, T. Saito, H. Kitazawa
    • Organizer
      ADSA-ASAS Joint Meeting
    • Place of Presentation
      Minneapolis, USA
    • Year and Date
      20060709-13
  • [Presentation] Immunobiotic lactic acid bacteria induce immune responses in immature gut-associated lymphoid tissues via Toll-like receptors 2 and 92006

    • Author(s)
      Kitazawa, H., M. Tohno, T. Shimosato, H. Aso, Y. Kawai, T. Saito
    • Organizer
      ADSA-ASAS Joint Meeting
    • Place of Presentation
      Minneapolis, USA
    • Year and Date
      20060709-13
  • [Book] プロバイオティクスからイムノバイオティクス~生体認識機構の解明から期待される世界的発展性~、「世界の発酵乳」分担2008

    • Author(s)
      北澤春樹、遠野雅徳
    • Total Pages
      152-171
    • Publisher
      はる書房
  • [Patent(Industrial Property Rights)] Drug and food composition for enhancing immune responce conaining AT oligonucleotide as the active ingredient2006

    • Inventor(s)
      Kitazawa, H, T. Saito, T. Shimosato, T. Sasaki, Y. Ito
    • Industrial Property Rights Holder
      明治ホ-ルディングス(株)
    • Industrial Property Number
      国際特許, WO 2006/093118 A1
    • Filing Date
      2006-09-08
    • Overseas

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Published: 2010-06-10   Modified: 2016-04-21  

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