Study on the network in ovary-uterus-immune systems in regulating ovulation cycle

2008 Fiscal Year Final Research Report

• Project/Area Number: 18380166
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Okayama University
• Principal Investigator: OKUDA Kiyoshi Okayama University, 大学院・自然科学研究科, 教授 (40177168)
• Co-Investigator(Kenkyū-buntansha): ACOSTA Tj 岡山大学, 大学院・自然科学研究科, 准教授 (80379718)
• Research Collaborator: SKARZYNSKI Dariusz J ポーランド科学アカデミー, 教授
• Project Period (FY): 2006 – 2008
• Keywords: 繁殖 / 代謝・内分泌制御 / 機能性物質

Research Abstract

本研究により、黄体で合成されるPGF2a、PGE2、コルチゾール、アセチルコリンが細胞死抑制因子として黄体機能の維持に作用すること、また、低酸素環境は黄体機能の衰退と消失(細胞死)に重要な環境要因であることが明らかにされた。また、コルチゾール、interleukin-1aは、子宮内膜のPGF2a合成制御因子として作用し、排卵周期の発現のみならず妊娠成立にも重要な役割を果たすことが示された

Research Products

• [Journal Article] Survival role of locally produced acetylcholine in the bovine corpus luteum (2009)
  • Author(s): Al-zi'abi MO, Bowolaksono A, Okuda K.
  • Journal Title: Biology of Reproduction 80 (4) Pages: 823-832
  • Peer Reviewed
• [Journal Article] Prostaglandin F2a stimulates 11b-hydroxysteroid dehydrogenase 1 enzyme bioactivity and protein expression in bovine endometrial stromal cells (2009)
  • Author(s): Lee HY, Acosta TJ, Skarzynski DJ, Okuda K.
  • Journal Title: Biology of Reproduction 80 (4) Pages: 657-664
  • Peer Reviewed
• [Journal Article] Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus (2008)
  • Author(s): Woclawek-Potocka I, Brzezicka E, Komiyama J, Siemieniuch M, Okuda K, Skarzynski DJ.
  • Journal Title: Reproduction 137(1) Pages: 95-105
  • Peer Reviewed
• [Journal Article] Anti-apoptptic roles of prostaglandin E2 and F2a in bovine luteal steridogenic cells (2008)
  • Author(s): Bowolaksono A, Nishimura R, Hojo T, Sakumoto R, Acosta TJ, Okuda K.
  • Journal Title: Biology of Reproduction 79(2) Pages: 310-317
  • Peer Reviewed
• [Journal Article] Urinary concentrations of estrone-3-glucuronide and estrous behavior in a captive female giant panda (Ailuropoda melanoleuca) (2008)
  • Author(s): Hama N, Kanemitsu H, Sakamoto K, Oyama Y, Acosta TJ, Ishikawa O, Pengyan W, Okuda K.
  • Journal Title: Journal of Reproduction and Development 54(4) Pages: 281-285
  • Peer Reviewed
• [Journal Article] The influence of tumor necrosis factor a (TNF) on the secretory function of bovine corpus luteum : TNF and its receptor expression during the estrous cycle (2008)
  • Author(s): Korzekwa A, Murakami S, Woclawek-Potocka I, Bah MM, Okuda K, Skarzynski DJ.
  • Journal Title: Reproductive Biology 8(3) Pages: 245-262
  • Peer Reviewed
• [Journal Article] Regulation of prostaglandin biosysynthesis by interleukin-1 in cultured bovine endometrial cells (2008)
  • Author(s): Tanikawa M, Lee HY, Watanabe K, Majewska M, Skarzynski DJ, Piotrowska KK, Park SB, Lee DS, Park CK, Acosta TJ, Okuda K.
  • Journal Title: Journal of Endocrinology 199 Pages: 425-434
  • Peer Reviewed
• [Journal Article] Cortisol is a suppressor of apoptosis in bovine corpus luteum (2008)
  • Author(s): Komiyama J, Nishimura R, Lee HY, Sakumoto R, Tetsuka M, Acosta TJ, Skarzynski DJ, Okuda K.
  • Journal Title: Biology of Reproduction 78(5) Pages: 888-895
  • Peer Reviewed
• [Journal Article] Hypoxia promotes luteal cell death in bovine corpus luteum (2008)
  • Author(s): Nishimura R, Komiyama J, Tasaki Y, Acosta TJ, Okuda K.
  • Journal Title: Biology of Reproduction 78(3) Pages: 529-536
  • Peer Reviewed
• [Journal Article] Changes in expression of 11・-hydroxysteroid dehydrogenase type-1, type-2 and glucocorticoid receptor mRNAs in porcine corpus luteum during the estrous cycle (2007)
  • Author(s): Sakumoto R, Ito I, Okuda K.
  • Journal Title: Molecular Reproduction and Development 75(5) Pages: 925-930
  • Peer Reviewed
• [Journal Article] Gene silencing of cyclooxygenase-2 mRNA by RNA interference in bovine cumulus-granulosa cells (2007)
  • Author(s): Kobayashi S-I, Sakatani M, Kobayashi S, Okuda K, Takahashi M.
  • Journal Title: Journal of Reproduction and Development 53(6) Pages: 1305-1311
  • Peer Reviewed
• [Journal Article] Expressions of estrogen receptors in bovine corpus luteum : cyclic changes and effects of prostaglandin F2・ and cytokines (2007)
  • Author(s): Shibaya M, Matsuda A, Hojo T, Acosta TJ, Okuda K.
  • Journal Title: Journal of Reproduction and Development 53(5) Pages: 1059-1068
  • Peer Reviewed
• [Journal Article] Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells (2007)
  • Author(s): Skarzynski DJ, Shibaya M, Tasaki Y, Korzekwa A, Murakami S, Woclawek-Potocka I, Majewska M, Okuda K.
  • Journal Title: Reproductive Biology 7(1) Pages: 3-15
  • Peer Reviewed
• [Journal Article] Infusion of exogenous tumor necrosis factor dose dependently alters the length of the luteal phase in cattle : differential response to treatment with indomethacin and L-NAME, a nitric oxide synthase inhibitor (2007)
  • Author(s): Skarzynski DJ, Woclawek-Potocka I, Korzekwa A, Bah MM, Piotrowska K, Barszczewska B, Okuda K.
  • Journal Title: Biology of Reproduction 76(4) Pages: 619-627
  • Peer Reviewed
• [Journal Article] The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium (2007)
  • Author(s): Lee HY, Acosta TJ, Tanikawa M, Sakumoto R, Komiyama J, Tasaki Y, Piskula M, Skarzynski DJ, Tetsuka M, Okuda K.
  • Journal Title: Journal of Endocrinology 193(1) Pages: 127-135
  • Peer Reviewed
• [Journal Article] Effects of storage and passage of luteal endothelial cells on endothelin-1 and prostaglandin F2・ production (2007)
  • Author(s): Acosta TJ, Yoshioka S, Komiyama J, Lee SH, Grazul-Bilska AT, Skarzynski DJ, Okuda K.
  • Journal Title: Journal of Reproduction and Development 53(3) Pages: 473-480
  • Peer Reviewed
• [Journal Article] Phyto- and endogenous estrogens differently activate intracellular calcium ion mobilization in the bovine endometrial cells (2006)
  • Author(s): Woclawek-Potocka I, Borkowski K, Korzekwa A, Okuda K, Skarzynski DJ.
  • Journal Title: Journal of Reproduction and Development 52(6) Pages: 731-740
  • Peer Reviewed
• [Journal Article] Nitric oxide induces apoptosis in bovine luteal cells (2006)
  • Author(s): Korzekwa A, Okuda K, Woclawek-Potocka I, Murakami S, Skarzynski DJ.
  • Journal Title: Journal of Reproduction and Development 52(3) Pages: 353-361
  • Peer Reviewed
• [Journal Article] Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum (2006)
  • Author(s): Nishimura R, Sakumoto R, Tatsukawa Y, Acosta TJ, Okuda K.
  • Journal Title: Endocrinology 147(9) Pages: 4273-80
  • Peer Reviewed
• [Journal Article] Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells (2006)
  • Author(s): Tatsukawa Y, Bowolaksono A, Nishimura R, Komiyama J, Acosta TJ, Okuda K.
  • Journal Title: Journal of Reproduction and Development 52(4) Pages: 517-22
  • Peer Reviewed
• [Journal Article] Equol and para-ethyl-phenol stimulate prostaglandins F2・ in bovine corpus luteum (2006)
  • Author(s): Woclawek-Potocka I, Bober A, 23. Korzekwa A, Okuda K, Skarzynski DJ.
  • Journal Title: Prostaglandins & other Lipid Mediators 79 Pages: 287-297
  • Peer Reviewed
• [Journal Article] Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle (2006)
  • Author(s): Sakumoto R, Komatsu T, Kasuya E, Saito T, Okuda K.
  • Journal Title: Domestic Animal Endocrinology 31(3) Pages: 46-257
  • Peer Reviewed
• [Journal Article] Role of intraluteal prostaglandin F2・, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum (2006)
  • Author(s): Bah MM, Acosta TJ, Pilawski W, Deptula KM, Okuda K, Skarzynski DJ.
  • Journal Title: Prostaglandins & other Lipid Mediators 79 Pages: 218-229
  • Peer Reviewed
• [Journal Article] Bovine corpus luteum is an extrapituitary site of prolactin production (2006)
  • Author(s): Shibaya M, Murakami S, Tatsukawa Y, Skarzynski DJ, Acosta TJ, Okuda K.
  • Journal Title: Molecular Reproduction and Development 73 Pages: 512-519
  • Peer Reviewed
• [Journal Article] Acute changes in circulating concentrations of progesterone, nitric oxide and partial pressure of oxygen during prostaglandin F2α induced luteolysis in cattle
  • Author(s): Acosta TJ, Bah MM, Korzekwa A, Woclawek-Potocka I, Markiewicz W, Jaroszewski JJ, Okuda K, Skarzynski DJ.
  • Journal Title: Journal of Reproduction and Development (in press)
  • Peer Reviewed
• [Journal Article] Effects of exogenous tumonecrosis factor-a on the secretory function of the bovine reproductive tract depend on tumor necrosis factor-1 concentrations
  • Author(s): Skarzynski DJ, Piotrowska KK, Bah MM, Korzekwa A, Woclawek-Potocka I, Sawai K, Okuda K.
  • Journal Title: Reproduction in Domestic Animals (in press)
  • Peer Reviewed
• [Presentation] ウシの発情周期発現機構における子宮の役割 (2009)
  • Author(s): 奥田潔
  • Organizer: 第110回日本畜産学会シンポジウム「ルミナントバイオロジーの進展開」招待講演
  • Place of Presentation: 神奈川
  • Year and Date: 20090327-29
• [Presentation] ウシ黄体細胞におけるアポトーシス抑制因子としての黄体形成ホルモン (LH) の役割 (2008)
  • Author(s): owolaksono A, 西村亮, Acosta TJ, 奥田潔
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20080918-20
• [Presentation] ウシ黄体における発情周期を通じた血管分布変化および中期黄体内血管分布に及ぼす PGF2α の影響 (2008)
  • Author(s): 法上拓生, Al-zi'abi MO, Acosta TJ, 奥田潔
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20080918-20
• [Presentation] Possible roles of hypoxia in luteal formation and regression in cattle (2008)
  • Author(s): Okuda K
  • Organizer: V Jubilee Congress of the Society for Biology of Reproduction
  • Place of Presentation: Worclaw, Poland
  • Year and Date: 20080910-13
• [Presentation] Prostaglandin F2α stimulates 11β-hydroxysteroid dehydrogenase type1 activity and protein expression in bovine endometrial stromal cells (2008)
  • Author(s): Lee HY, Acosta TJ, Okuda K
  • Organizer: 41th Annual Meeting Society for the Study of Reproduction
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080527-30
• [Presentation] Hypoxia plays important roles not only in leteolysis but in luteal formation in cattle (2008)
  • Author(s): Okuda K, Nishimura R Acosta TJ
  • Organizer: First World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20080524-26
• [Presentation] ウシ子宮内膜の glucocorticoid receptor α および 11β-hydroxysteroid dehydrogenases protein 発現に及ぼす cortisol の影響 (2008)
  • Author(s): 李和容, Acosta TJ, 奥田潔
  • Organizer: 第145回日本獣医学会学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 20080328-30
• [Presentation] ウシ黄体の形成および退行における低酸素環境の意義 (2008)
  • Author(s): 西村亮, 小見山純一, Acosta TJ, 奥田潔
  • Organizer: 第145回日本獣医学会学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 20080328-30
• [Presentation] 低酸素環境はウシ黄体細胞のプロジェステロン合成を抑制することによりアポトーシスを誘導する (2007)
  • Author(s): 西村亮, Acosta TJ, 奥田潔
  • Organizer: 第100回日本繁殖生物学会大会
  • Place of Presentation: 東京
  • Year and Date: 20071020-22
• [Presentation] Prostaglandin (PG) F2・ は 11・-hydroxysteroid dehydrogenase (11HSD) 1 活性 および protein 発現を刺激する (2007)
  • Author(s): 李和容, Acosta TJ, 小見山純一, 田崎ゆかり, 奥田潔
  • Organizer: 第100回日本繁殖生物学会大会
  • Place of Presentation: 東京
  • Year and Date: 20071020-22
• [Presentation] Immunohistochemical study on cFLIP in the bovine corpus luteum (2007)
  • Author(s): Al-zi'abi O, Hojo T, Matsuda-Minehata F, Acosta TJ, Manabe N, Okuda K
  • Organizer: 第108回日本畜産学会
  • Place of Presentation: 岡山
  • Year and Date: 20070926-27
• [Presentation] Possible anti-apoptotic roles of prostaglandins in bovine luteal cells (2007)
  • Author(s): Okuda K, Bowolaksono A, Nishimura R, Sakumoto R, Komiyama J, Acosta TJ
  • Organizer: 40th Annual Meeting Society for the Study of Reproduction
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20070721-25
• [Presentation] 低酸素環境による黄体退行誘導メカニズムの解析 (2006)
  • Author(s): 西村亮, 作本亮介, Acosta TJ, 奥田潔
  • Organizer: 第99回日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 20060907-09
• [Presentation] ウシ黄体由来血管内皮細胞における一酸化窒素 (NO) 合成酵素 mRNA 発現 (2006)
  • Author(s): Acosta TJ, 吉岡伸, 小見山純一, 西村亮, Skarzynski DJ, 奥田潔
  • Organizer: 第99回日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 20060907-09
• [Presentation] ウシ黄体における aromatase 及び estrogen receptor 発現に及ぼす prostaglandin F2・ の影響 (2006)
  • Author(s): 柴谷雅美, Acosta TJ, 奥田潔
  • Organizer: 第99回日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 20060907-09
• [Presentation] ウシ子宮内膜における発情周期を通じた 11・-hydroxysteroid dehydrogenase (11HSD) 1 活性とその活性におよぼす PGF2・ の影響 (2006)
  • Author(s): 李和容, Acosta TJ, 西村亮, 小見山純一, 田崎ゆかり, 作本亮介, 手塚雅文, 奥田潔ウシ子宮内膜における発情周期を通じた 11・-hydroxysteroid dehydrogenase (11HSD) 1 活性とその活性におよぼす PGF2・ の影響
  • Organizer: 第99回日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 20060907-09
• [Presentation] ウシ黄体における11・-hydroxysteroid dehydrogenase type1 (11HSD1) 活性および glucocorticoid (2006)
  • Author(s): 小見山純一, 西村亮, 李和容, 作本亮介, 手塚雅文, Acosta TJ, 奥田潔
  • Organizer: (GC) の作用機序. 第99回日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 20060907-09
• [Presentation] Survival factors against apoptosis in bovine corpus luteum (2006)
  • Author(s): Okuda K, Bowolaksono A, Nishimura R, Sakumoto R, Acosta TJ
  • Organizer: 39th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Omaha, USA
  • Year and Date: 20060729-0801
• [Presentation] Oxygen concentration is an important factor for luteolysis in cattle (2006)
  • Author(s): Nishimura R, Sakumoto R, Acosta TJ, Okuda K
  • Organizer: 39th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Omaha, USA
  • Year and Date: 20060729-0801
• [Presentation] Effects of glucocorticoid on prostaglandin production in bovine endometrium (2006)
  • Author(s): Lee HY, Acosta TJ, Sakumoto R, Tetsuka M, Okuda K
  • Organizer: 39th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Omaha, USA
  • Year and Date: 20060729-0801