2009 Fiscal Year Final Research Report
Mechanism of light sensing and signaling mediated by phytochrome and its interacting proteins in plants
| Project/Area Number |
18380200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
KOHCHI Takayuki Kyoto University, 大学院・生命科学研究科, 教授 (40202056)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMATO T.Katsuyuki 京都大学, 大学院・生命科学研究科, 助教 (50293915)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 発生・分化制御 / タンパク質間相互作用 / 植物光信号伝達 / 光受容体 / フィトクロム / 環境応答 |
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| Research Abstract |
The novel zinc finger proteins were identified by protein interaction screening with phytochromes. The mutants having T-DNA insertion in the corresponding genes showed late flowering phenotype. The genetic analysis indicated the genes regulated FT expression as downstream factors of phytochrome B. The studies of temporal and spatial expression, phenotypic complementation with the modified genes indicated the proteins functioned in the nucleus in vascular tissue. Our finding gives insight on the link between light environment and development in growth control.
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[Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.2006
Author(s)
Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., Kohchi, T.
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Journal Title
FEBS Lett. 580
Pages: 1333-1338
Peer Reviewed
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[Journal Article] Reconstitution of blue/green reversible photoconversion of a cyanobacterial photoreceptor. PixJ1 in phycocyanobilin producing E. coli.2006
Author(s)
Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
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Journal Title
Biochem. 45
Pages: 3775-3784
Peer Reviewed
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