2007 Fiscal Year Final Research Report Summary
Development of Dual-Tandem-Mass Spectrometer with a New Ion-trap and Its Application to Analysis of Molecular Nano-dynamics of a Cell
| Project/Area Number |
18390014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
MASUJIMA Tsutomu Hiroshima University, Graduate School of Biomed. Sci, Professor (10136054)
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| Co-Investigator(Kenkyū-buntansha) |
TSUYAMA Naohiro Hiroshima Univ, Graduate School of Biomed. Sci, Assis. Prof (10335747)
MIZUNO Hajime Hiroshima Univ, Graduate School of Biomed. Sci, Res. Assoc (30457288)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Mass Spectrometer / Ion Trap / Cell Molecular Analysis / Time of Flight Mass Spectrometer / Quadrupole Mass Spectroteter |
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| Research Abstract |
1. Development of Dual-Tandem-Mass Spectrometer with New Ion-trap
A new concept of tripole was simulated by computer and it was found that this new tripole can be used not only for mass filter but also for a new gap ion trap which consists of two tripoles. With these new ion optics, a new dual tandem mass spectrometer which has two mass filter, one collision cell and one time-of-flight mass spectrometer, by which we can analyze molecules in quantitative and qualitative ways. The test machine was developed and its performance was checked.
2. Live Single Cell Mass Spectrometric Analysis
With such sensitive and versatile new mass spectrometer, we can apply this type of mass spectrometer for live single cell analysis. However, the molecular amount of a single cell is very tiny with a million molecules for each molecular species. Thus it has not been succeeded before. We finally found a new super sensitive ionization methodology in which we inserted a nano-electro spray tip into a single cell under video-microscope and suck the contents of a cell and the tip was directly used as a nano-electro spray ionization source. This method is so successful to introduce the molecular contents of a cell and to detect hundreds of molecular peaks.
This new method will be used in every aspect of bio-sciences and will accelerate the speed of analyses of life sciences.
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[Journal Article] Resin-packed nanoelectrospray in combination with ideo and mass spectrometry for the direct and timely molecular analysis of mast cells2007
Author(s)
Sirikatitham, S., Yamamoto, T., Shimizu, M., Hasegawa, T., Tsuyama, N., Masujima, T
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Journal Title
Rapid Commun. Mass Spectrom 21
Pages: 385-390
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nanokinetics of drug molecule transport into a single cell2006
Author(s)
Tamura, A., Ozawa, K., Ohya, T., Tsuyama, N., Eyring, E., Masujima, T
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Journal Title
Nanomedicine 1
Pages: 345-350
Description
「研究成果報告書概要(欧文)」より
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