2009 Fiscal Year Final Research Report
Study on the formation and function of exosomes
| Project/Area Number |
18390032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
NISHIJIMA Masahiro National Institute of Health Sciences, 所長 (60072956)
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| Co-Investigator(Kenkyū-buntansha) |
川崎 清史 同志社女子大学, 薬学部, 准教授 (60270641)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 脂質 / 細菌 / エンドトキシン / 質量分析 / 細胞内増殖 |
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| Research Abstract |
Exosomes are composed of proteins and lipids, which are secreted from cells including those infected with bacteria. In order to examine the structure of lipid A portion of LPS, which is potentially involved in the exosomes, we developed a method for mass spectrometry analysis of lipid A. We found that 2, 5-dihydroxybenzoic acid matrix was preferable for the detection of aminoarabinose modification. Salmonella Typhimurium LpxR potentially detoxifies lipid A by 3'-O-deacylation ; however, the involvement of deacylation in its adaptation to host cells remains unclear. Intracellular replication assay demonstrated that growth of the lpxR-null strain was lower than that of the wild-type strain, indicating that lipid A 3'-O-deacylation is beneficial for intracellular growth.
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