2009 Fiscal Year Final Research Report
Analysis of endosome/post-endosome system with special reference to water channel aquaporin 2
| Project/Area Number |
18390057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Gunma University |
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Principal Investigator |
TAKATA Kuniaki Gunma University, 学長 (20129290)
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Haruo 群馬大学, 大学院・医学系研究科, 准教授 (80189464)
AOKI Takeo 群馬大学, 大学院・医学系研究科, 講師 (70150919)
SUZUKI Takeshi 群馬大学, 大学院・医学系研究科, 助教 (00261868)
MATSUZAKI TOSHIYUKI 日本医科大学, 医学部, 准教授 (30334113)
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| Project Period (FY) |
2006 – 2009
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| Keywords | アクアポリン2 / エンドソーム / 小胞 / トラッフィッキング / 水輸送 |
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| Research Abstract |
Vesicular trafficking that determines the number of cell surface molecules serves as one of important regulatory mechanisms found in the control of the reabsorption of water in the kidney, maintenance of blood glucose level, etc.Intracellular vesicles involved in the endocytosis and subsequent recycling process, such as endosomes and post-endosomal vesicles are key components in such vesicular trafficking and storage. We used water channel aquaporin 2 whose trafficking is regulated by hypophysial hormone vasopressin. Glucose transporter 4(GLUT4), whose trafficking is regulated by insulin in adipocytes and muscle cells, was coexpressed with aquaporin 2 in MDCK cells, and their localization was visualized by immunofluorescence and GFP-tagging methods. Aquaporin 2 and GLUT4 exhibited distinct localization pattern each other in both the resting and stimulated states, showing that aquaporin 2 and GLUT4 are stored in distinct post-endosomal compartments each other, and that their trafficking is differentially regulated. Translocation of aquaporin 2 has been considered to be regulated by its phosphorylation. We raised antibodies that specifically detect phosphorylated aquaporin 2. By western blotting and immunofluorescence microscopy, phosphorylation of aquaporin 2 was found not only on the cell surface but also intracellular vesicles, suggesting the ubiquitous presence of phosphorylated aquaporin 2.
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[Journal Article] Long-term forskolin stimulation induces AMPK activation and thereby enhances tight junction formation in human placental trophoblast BeWo cells2008
Author(s)
Egawa M, Kamata H, Kushiyama A, Sakoda H, Fujishiro M, Horike N, Yoneda M, Nakatsu Y, Ying G, Jun Z, Tsuchiya Y, Takata K, Kurihara H, Asano T
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Journal Title
Placenta 29
Pages: 1003-1008
Peer Reviewed
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