The regulatory mechanism of sensor-type transcription factor during mitosis

2008 Fiscal Year Final Research Report

• Project/Area Number: 18390083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Shimane University (2007-2008); Nagoya University (2006)
• Principal Investigator: URANO Takeshi Shimane University, 医学部, 教授 (70293701)
• Project Period (FY): 2006 – 2008
• Keywords: 分裂期キナーゼ / Aurora / p53 / リン酸化モノクローナル抗体

Research Abstract

細胞分裂期の染色体分配など複雑な物理的ストレスをがん細胞が回避する機構を解明する端緒として,様々なストレスに反応する転写因子であるp53 およびその下流の実働分子であるp21/WAF1 が細胞分裂期にAurora によりリン酸化され,それらの機能が細胞分裂期特異的に不活性化されていることを見出した。

Research Products

• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin. (2009)
  • Author(s): Shimada A, Dohke K, Sadaie M, Shinmyozu K,Nakayama J, Urano T and Murakami Y
  • Journal Title: Genes Dev. 23 Pages: 18-23
  • Peer Reviewed
• [Journal Article] Glycosylphosphatidylinositol-anchored arginine-specific ADP-ribosyltransferase7.1(Art7.1) on chicken B cells: the possible role of Art7 in B cell receptor signalling and proliferation. (2009)
  • Author(s): Terashima M, Takahashi M, Shimoyama M,Tanigawa Y, Urano T and Tsuchiya M
  • Journal Title: Mol Cell Biochem 320 Pages: 470-478
  • Peer Reviewed
• [Journal Article] Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation. (2008)
  • Author(s): Kondo Y, Shen L, Cheng AS, Ahmed S,Boumber Y, Charo C, Yamochi T, Urano T,Furukawa K, Kwabi-Addo B, Gold DL,Sekido Y, Huang TH and Issa JP
  • Journal Title: Nature Genet 40 Pages: 741-750
  • Peer Reviewed
• [Journal Article] Heterochromatin and RNAi Are Required to Establish CENP-A Chromatin at Centromeres. (2008)
  • Author(s): Folco HD, Pidoux AL, Urano T, and Allshire RC
  • Journal Title: Science 319 Pages: 94-97
  • Peer Reviewed
• [Journal Article] Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast. (2008)
  • Author(s): Shimada M, Yamada-Namikawa C,Murakami-Tonami Y, Yoshida T, Nakanishi M, Urano T, and Murakami H
  • Journal Title: EMBO J 27 Pages: 132-142
  • Peer Reviewed
• [Journal Article] Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin. (2008)
  • Author(s): Dohke K, Miyazaki S, Tanaka K, Urano T,Grewal SI and Murakami Y
  • Journal Title: Genes Cells 13 Pages: 1027-1043
  • Peer Reviewed
• [Journal Article] The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin. (2007)
  • Author(s): Trewick SC, Minc E, Antonelli R, Urano T,and Allshire RC
  • Journal Title: EMBO J. 26 Pages: 4670-4682
  • Peer Reviewed
• [Journal Article] Gene amplification and overexpression of PRDM14 in breast cancers. (2007)
  • Author(s): Nishikawa N, Toyota M, Suzuki H,Honma T, Fujikane T, Ohmura T,Nishidate T, Ohe-Toyota M, Maruyama R,Sonoda T, Sasaki Y, Urano T, Imai K,Hirata K, and Tokino T
  • Journal Title: Cancer Res 67 Pages: 9649-9657
  • Peer Reviewed
• [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensin I. (2007)
  • Author(s): Takemoto A, Murayama A, Katano M,Urano T, Furukawa K, Yokoyama S,Yanagisawa J, Hanaoka F and Kimura K
  • Journal Title: Nucleic Acids Res 35 Pages: 2403-2412
  • Peer Reviewed
• [Journal Article] Role of DNA methylation and histone H3 Lysine 27 methylation in tissue-specific imprinting of mouse Grb10. (2007)
  • Author(s): Yamasaki-Ishizaki Y, Kayashima T,Mapendano CK, Soejima H, Ohta T,Masuzaki H, Kinoshita A, Urano T,Yoshiura KI, Matsumoto N, Ishimaru T,Mukai T, Niikawa N and Kishino T
  • Journal Title: Mol. Cell. Biol. 27 Pages: 732-742
  • Peer Reviewed
• [Journal Article] Overexpression of caveolin-1 in a human melanoma cell line results in dispersion of ganglioside GD3 from lipid rafts and alteration of leading edges, leading to attenuation of malignant properties. (2007)
  • Author(s): Nakashima H, Hamamura K, Houjou T,Taguchi R, Yamamoto N, Mitsudo K,Tohnai I, Ueda M, Urano T, Furukawa K,Furukawa K
  • Journal Title: Cancer Sci 98 Pages: 512-520
  • Peer Reviewed
• [Journal Article] Phospho-regulation of human Protein Kinase Aurora-A: Analysis using anti-phospho-Thr288 monoclonal antibodies. (2006)
  • Author(s): Ohashi S, Sakashita G, Ban R, Nagasawa M, Matsuzaki H, Murata Y, Taniguchi H,Shima H, Furukawa K and Urano T
  • Journal Title: Oncogene 25 Pages: 7691-7702
  • Peer Reviewed
• [Journal Article] Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. (2006)
  • Author(s): Goto H, Kiyono T, Tomono Y, Kawajiri A, Urano T, Furukawa K, Nigg EA and Inagaki M
  • Journal Title: Cell Biol 8 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Nucleophosmin (NPM): A versatile molecule associated with hematological malignancies. (2006)
  • Author(s): Naoe T, Suzuki T, Kiyoi H and Urano T
  • Journal Title: Cancer Sci 97 Pages: 963-969
• [Journal Article] Expression profile of LIT1/KCNQ1OT1 and epigenetic status at the KvDMR1 in colorectal cancers. (2006)
  • Author(s): Nakano S, Murakami K, Meguro M, Soejima H, Higashimoto K, Urano T,Kugoh H, Mukai T, Ikeguchi M and Oshimura M
  • Journal Title: Cancer Sci 97 Pages: 1147-1154
  • Peer Reviewed
• [Journal Article] Targeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to verotoxins. (2006)
  • Author(s): Okuda T, Tokuda N, Numata S, Ito M, Ohta M, Kawamura K, Wiels J, Urano T, Tajima O, Furukawa K and Furukawa K
  • Journal Title: J. Biol. Chem 281 Pages: 10230-10235
  • Peer Reviewed
• [Journal Article] Metastatic potential of mouse Lewis lung cancer cells is regulated via ganglioside GM1 by modulating the matrix metalloprotease-9 localization in lipid rafts. (2006)
  • Author(s): Zhang Q, Furukawa K, Chen HH, Sakakibara T, Urano T and Furukawa K
  • Journal Title: J. Biol. Chem 281 Pages: 18145-18155
  • Peer Reviewed
• [Presentation] 細胞周期を制御する分裂期キナーゼAurora (2007)
  • Author(s): 浦野 健
  • Organizer: 第16回日本アポトーシス研究会
  • Place of Presentation: 千葉
  • Year and Date: 2007-08-04
• [Presentation] Calcium/Calmodulin Binds to and Inhibits Aurora-A Kinase Activity at Anaphase (2000)
  • Author(s): Reiko Ban and Takeshi Urano
  • Organizer: 48^<th> ASCB
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 200081214
• [Book] 細胞周期集中マスター 北川雅敏編、-レビュー編第1 章細胞周期を制御するリン酸化酵素- (2006)
  • Author(s): 浦野 健
  • Total Pages: 48-62
  • Publisher: 株式会社羊土社
• [Remarks] ホームページ等
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html