2007 Fiscal Year Final Research Report Summary
Molecular mechanisms of the maintenance of stem cell systems
| Project/Area Number |
18390088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
NAKANO Toru Osaka University, Graduate Schal of Frontier Biosziences, Professor (00172370)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Stem cells / ES cells / Signal transduction / Blood cells / Cell differentiation / Reprogramming / Regenerative medicine |
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| Research Abstract |
To analyze the molecular mechanisms of the maintenance of stem cell immaturity and those of the cell differentiation in stem cell systems, we carried out the following research.
1) Function of Akt signal in the maintenance of embryonic stem cells
Nuclear reprogramming' can take place by cell fusion between somatic cells with mouse embryonic stem calls (ES cells). Activation of Akt signaling in ES cells significantly promoted the efficiency of the reprogramming. In addition, the cells obtained by the cell fusion acquired the pluripotency Them results clearly demonstrate that Akt signal can maintain the pluripotency of the cells. To the contrary, Akt signaling significantly reduced the efficiency of reprogramming after somatic nuclear transfer.
2) Reprogramming of blood cell differentiation by the null mutation of GATA-1
GATA-1 is an essential transcription factor for erythroid differentiation. In vitro differentiation from GATA-1 null ES cells to erythroid cells gave rim to the erythroid cells. And the erythroid nails mild proliferate for long term. These erythroid cells possessed the ability to differentiate to neutrophils and macrophages. In addition, epigenetic status of the GATA-1 null erythroid cells was quite different from its wild type counterpart. This result clearly demonstrate that this experimental system is an excellent model system to analyze the epigenetic modification by transcription factors.
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[Journal Article] Akt signaling promotes derivation of embryonic germ cells from primordial germ cells2008
Author(s)
Kimura, T, Tomooka, M, Yamano, N, Murayama, K, Matoba, S, Umehara, H, Kanai, Y, Nakano,_T
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Journal Title
Development 135
Pages: 869-79
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differential context-dependent effects of FOG-1 on mast cell development and differentiation2008
Author(s)
Sugiyama, D, Tanaka, M, Kitajima, K, Zheng, J, Yen, H, Murotani, T, Yamatodani, A, Nakano, T
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Journal Title
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「研究成果報告書概要(欧文)」より
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[Journal Article] PGC7/Stella protects against DNA demethylation in early embryogenesis2007
Author(s)
Nakamura, T, Arai, Y, Umehara, H, Masuhara, M, Kimura, T, Taniguchi, H, Sekimoto, T, Ikawa, M, Yoneda, Y, Okabe, M, Tanaka, S, Shiota, K, Nakano, T
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Journal Title
Nature Cell Biol 9
Pages: 64-71
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Akt activation induces epidermal hyperplasia and proliferation of epiderthal progenitors2007
Author(s)
Murayama, K, Kimura, T, Tarutani, M, Tomooka, M, Hayashi, R, Okabe, M, Nishida, K, Itami, S, Katavama, I, Nakano, T
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Journal Title
Oncogene 26
Pages: 4882-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Pten/PI3K pathway governs the homeostasis of Valphal4iNKT cells2007
Author(s)
Kishimoto, H, Ohteki, T, Yajima, N, Kawahara, K, Natsui, M, Kawarasaki, S, Hamada, K, Horie, Y, Kubo, Y, Arase, S, Taniguchi, M, Vanhaesebroeck, B, Mak, TW., Nakano, T., Kovasu, S., Sasaki, T., Suzuki
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Journal Title
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「研究成果報告書概要(欧文)」より
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[Journal Article] Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-32007
Author(s)
Umehara, H, Kimura, T, Ohtsuka, S, Nakamura, T, Kitajima, K, Ikawa, M, Okabe, M, Niwa, H, Nakano, T
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Journal Title
Stem Cells 25
Pages: 2705-11
Description
「研究成果報告書概要(欧文)」より
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