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2007 Fiscal Year Final Research Report Summary

Molecular mechanism of circadian oscillation by real-time rhythm monitoring

Research Project

Project/Area Number 18390093
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOsaka Bioscience Institute

Principal Investigator

TAKUMI Toru  Osaka Bioscience Institute, Neuroscience, Lab Head (00222092)

Co-Investigator(Kenkyū-buntansha) TAKANO Atsuko  Osaka Bioscience Institute, Neuroscience, 研究員 (70390812)
Project Period (FY) 2006 – 2007
Keywordsgene / gene expression / behavior / physiology
Research Abstract

The circadian expression of the mammalian clock genes is based on transcriptional feedback loops. Two bHLH-PAS domain-containing transcriptional activators, CLOCK and BMAL1, are known to regulate gene expression by interacting with a promoter element termed the E-box (CACGTG). The non-canonical E-boxed or E-box-like sequences have also been reported to be necessary for circadian oscillation. We found a new cis-element required for cell-autonomous circadian transcription of cock genes. This new element consists of a canonical E-box or a non-canonical E-box and an E-box-like sequence in tandem with the latter with a short interval, 6 base pairs, between them. We demonstrated that both E-box or E-box-like sequences are needed to generate cell-autonomous oscillation. We also verified that the spacing nucleotides with constant length between these 2 E-elements are crucial for robust oscillation. Thus, the direct repeat of the E-box-like elements is the minimal required element for the generation of cell-autonomous transcriptional oscillation of clock and clock-controlled genes.
On the other hand, we developed in vivo monitoring of circadian timing in freely moving mice by recording multi-unit neural activity (MIJA). Suprachiasmatic nucleus (SCN), the mammalian circadian center, neural activity is tightly coupled to environmental photic input and anticorrelated with MUA rhythm in the subparaventricular zone (SPZ). In Clock mutant mice exhibiting attenuated circadian locomotor rhythmicity, MUA rhythmicity in the SCN and SPZ is similarly blunted. These results suggest that the SPZ plays a functional role in relaying circadian and photic signals to centers involved in generating behavioral activity.

  • Research Products

    (34 results)

All 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 6 results) Presentation (16 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Of mice and men-universality and breakdown of behavioral organization.2008

    • Author(s)
      Nakamura T
    • Journal Title

      PLoS ONE 3

      Pages: e2050

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] In vivo monitoring of circadian timing in freely moving mice2008

    • Author(s)
      Nakamura W
    • Journal Title

      Curr Biol 18

      Pages: 381-385

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A direct repeat of E-box-like element is required for cell-autonomous circadian rhythm of clock genes.2008

    • Author(s)
      Nakahata Y
    • Journal Title

      BMC Mol Biol 9

      Pages: 1

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Of mice and men-universalit and breakdown of behavioral organization2008

    • Author(s)
      Nakamura T, Takumi, T., Takano, A., Aoyagi, N., Yoshiuchi, K., Struzik, ZR., Yamamoto, Y.
    • Journal Title

      PLoS ONE (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vivomonitoring of circadian timing in freely moving mice.2008

    • Author(s)
      Nakamura, W., Yamazaki, S., Nakamura, TJ., Shirakawa, T., Block, GD., Takumi, T.
    • Journal Title

      Curr. Biol. 18

      Pages: 381-385

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A direct repeat of E-box-like element is required for cell-autonomous circadian rhythm of clock genes.2008

    • Author(s)
      Nakahata, Y., Yoshida, M., Takano, A., Soma, H., Yamamoto, T., Yasuda, A., Nakatsu, T., Takumi, T.
    • Journal Title

      BMC Mol Biol. 9

      Pages: 1

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 時計遺伝子の転写振動及びその分子メカニズム2007

    • Author(s)
      内匠 透
    • Journal Title

      自律神経 44

      Pages: 246-250

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] リチウムと概日時計とうつ病2007

    • Author(s)
      内匠 透
    • Journal Title

      Clinical Neuroscience 25

      Pages: 1156-1157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular mechanism of cell-autonomous circadian gene expression of Period2, a crucial regulator of the mammalian circadian clock2006

    • Author(s)
      Akashi,M.,Ichise,T.,Mamine,T.,Fukui,K.and Takumi,T.
    • Journal Title

      Mol.Biol.Cell. 17

      Pages: 555-565

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The in vitro real-time oscillation monitoring system identifies potential entrainment factors for circadian clocks.2006

    • Author(s)
      Nakahata,Y.,Akashi,M.,Trcka,D.,Yasuda,A.and Takumi,T.
    • Journal Title

      BMC Mol Biol. 7

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Myosin-Va facilitates the accumulation of mRNA/Protein (mRNP) complex in dendritic spines.2006

    • Author(s)
      Yoshimura,A.,Fujii,R.,Watanabe,Y,Okabe,S.,Fukui,K.and Takumi,T.
    • Journal Title

      Curr.Biol. 16

      Pages: 2345-2351

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] からくり分子時計2006

    • Author(s)
      内匠 透
    • Journal Title

      細胞工学 25

      Pages: 174-178

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 分子時計の概日転写制御機構2006

    • Author(s)
      内匠 透
    • Journal Title

      生化学 78

      Pages: 425-429

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular mechanism of cell-autonomous circadian gene expression of Period2, a crucial regulator of the mammalian circadian clock2006

    • Author(s)
      Akashi, M., Ichise, T., Mamine, T., Fukui, K., Takumi, T.
    • Journal Title

      Mol. Biol. Cell. 17

      Pages: 555-565

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The in vitro rel-time oscillation monitoring system identifies potential entrainment factors for circadian clocks2006

    • Author(s)
      Nakahata, Y., Akashi, M. Trcka, D., Yasuda, A., Takumi, T.
    • Journal Title

      BMC Mol Biol. 7

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Myosin-Va facilitates the accumulation of mRNA/Protein(mRNP) complex in dendritic spines.2006

    • Author(s)
      Yoshimura, A., Fujii, R., Watanabe, Y., Okabe, S., Fukui, K., Takumi, T.
    • Journal Title

      Curr. Biol. 16

      Pages: 2345-2351

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular chronobiology using whole body2008

    • Author(s)
      Toru Takumi
    • Organizer
      第85回日本生理学会大会 シンポジウム「Approach to whole body: New stream to study autonomic nervous and neuroendocrine system
    • Place of Presentation
      東京
    • Year and Date
      20080325-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 生物時計とうつ状態との分子的相関2007

    • Author(s)
      高野敦子
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] in vitroリズムモニター系による時計遺伝子概日転写の分子機構の解明及び同調因子の探索2007

    • Author(s)
      松原千明
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 生物時計とうつ状態との分子的相関2007

    • Author(s)
      高野敦子、内匠透
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会 ワークショップ「生物時計分子機構と健康医療」
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular mechanism of biological clock and its output for circadian physiology and pathophysiology2007

    • Author(s)
      Toru Takumi
    • Organizer
      University of Surrey Workshop "Sleep, Circadian Rhythms and Cognition: Bridging the Genotype-Phenotype Gap"
    • Place of Presentation
      Guilford,UK
    • Year and Date
      20071128-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular mechanism of biological clock and its output for circadian physiology and pathophysiology2007

    • Author(s)
      Toru Takumi.
    • Organizer
      University of Surrey Workshop Sleep, Circadian Rhythms and Cognition : Bridging the Genotype-Phenotype Gap
    • Place of Presentation
      Guilford, UK
    • Year and Date
      20071128-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular interaction between depression and circadian behavior2007

    • Author(s)
      Atsuko Takano, Toru Takumi
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071111-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effect of prostaglandin J2(PGJ2)on the circadian rhythm of the rat C6 glioma cell2007

    • Author(s)
      Satoshi Koinuma, Kazuhiro Yagita, Naoto Hayasaka, Atsuko Fujioka, Yasukazu Nakahata, Toru Takumi, Yasufumi Shigeyoshi
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071111-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The blues and rhythm in the body and cells2007

    • Author(s)
      Toru Takumi.
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071111-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Circadian output in the suprachiasmatic nucleus2007

    • Author(s)
      Wataru Nakamura and Toru Takumi
    • Organizer
      日本睡眠学会第32回定期学術集会、第14回日本時間生物学会学術大会 シンポジウム「Functional structure of the suprachiasmatic nucleus」
    • Place of Presentation
      東京
    • Year and Date
      20071107-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular interaction between depression and circadian beahvior2007

    • Author(s)
      Atsuko Takano
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071104-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Rhythmic properties of the mouse suprachiasmatic necleus in vivo2007

    • Author(s)
      Wataru Nakamura
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071104-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effect of prostaglandin J2(PGJ2) on the circadian rhythm of the rat C6 glioma cell2007

    • Author(s)
      Satoshi Koinuma
    • Organizer
      2nd World Congress of Chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20071104-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The blues and rhythm in the body and cells2007

    • Author(s)
      Toru Takumi
    • Organizer
      2nd World Congress of Chronobiology Symposium "Mood disorders and biological clock"
    • Place of Presentation
      Tokyo
    • Year and Date
      20071104-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular Mechanism of Circadian Rhythm and Its Clinical Implication2007

    • Author(s)
      Toru Takumi
    • Organizer
      The 4th International Joint Symposium of Korea Biomedical Genomic Research Association and Center for Genome Science "Roads to Personalized and Preventive Medicine"
    • Place of Presentation
      Seoul,Korea
    • Year and Date
      2007-12-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular Mechanism of Circadian Rhythm and Its Clinical Implication2007

    • Author(s)
      Toru Takumi
    • Organizer
      The 4th International Joint Symposium of Korea Biomedical Genomic Research Association and Center for Genome Science Roads to Personalized and Preventive Medicine
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2007-12-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 体内時計はどこにある?-腹?脳?.NPO法人脳の世紀推進会議 編.2007

    • Author(s)
      内匠 透
    • Total Pages
      9-37
    • Publisher
      クバプロ
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 抗鬱薬スクリーニング系およびそれを用いるスクリーニング方法2006

    • Inventor(s)
      内匠 透
    • Industrial Property Rights Holder
      (財)大阪バイオサイエンス研究所
    • Industrial Property Number
      特願2006-168977
    • Filing Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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