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2008 Fiscal Year Final Research Report

Mechanism for intracellular action of bacterial modulin from intracellular parasitic bacteria and application

Research Project

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Project/Area Number 18390134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyoto University

Principal Investigator

MITSUYAMA Masao  Kyoto University, 医学研究科, 教授 (10117260)

Project Period (FY) 2006 – 2008
Keywords細胞内寄生菌 / 細菌性モジュリン / リステリア菌 / 結核菌 / サイトカイン / マクロファージ
Research Abstract

細胞内寄生性細菌であるリステリア菌および結核菌が有する細菌性モジュリンの感染細胞に対する作用を、主としてサイトカイン応答を中心として解析した。リステリアの病原因子LLOが感染マクロファージ内で認識されインフラマソーム形成が起こってカスペース1が活性化される分子機構や、結核菌が宿主カスペース1活性化を誘導する過程にRD1 領域産物が関与することなど、多くの新たな知見が得られた。

  • Research Products

    (35 results)

All 2009 2008 2007 2006 Other

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (10 results) Book (4 results) Remarks (1 results)

  • [Journal Article] RD1 locus in Mycobacterium tuberculosis genome contributes 1 to the activation of caspase-1 via induction of potassium ion 2 efflux in infected macrophages Infect Immu2009

    • Author(s)
      Takeshi Kurenuma, Ikuo Kawamura*, Hideki Hara, Ryosuke Uchiyama, Sylvia Daim, Sita Ramyamali Dewamitta, Shunsuke Sakai, Kohsuke Tsuchiya, Takamasa Nomura and Masao Mitsuyama
    • Journal Title

      submitted 6

    • Peer Reviewed
  • [Journal Article] プロバイオテイクスと疾患はじめに2009

    • Author(s)
      光山正雄
    • Journal Title

      医学のあゆみ 228(3)

      Pages: 197

    • Peer Reviewed
  • [Journal Article] Critical involvement of pneumolysin in production of IL-1 α and caspase-1- dependent cytokines in infection with Streptococcus pneumoniae in vitro: a novel function of pneumolysin in caspase-1 activation2008

    • Author(s)
      Shereen Shoma, Kohsuke Tsuchiya*, Ikuo Kawamura, Takamasa Nomura, Hideki Hara, Ryosuke Uchiyama, Sylvia Daim and Masao Mitsuyama
    • Journal Title

      Infection and Immunity 76(4)

      Pages: 1547-1557

    • Peer Reviewed
  • [Journal Article] Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, Listeriolysin O, after evasion from phagosome into the cytoplasm2008

    • Author(s)
      Hideki Hara, Kohsuke Tsuchiya, Takamasa Nomura, Ikuo Kawamura, Shereen Shoma and Masao Mitsuyama
    • Journal Title

      J Immunol 180

      Pages: 7859-7868

    • Peer Reviewed
  • [Journal Article] 感染制御2008

    • Author(s)
      光山正雄
    • Journal Title

      第81回日本細菌学会総会最新の話題 4(4)

      Pages: 313-315

    • Peer Reviewed
  • [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A) contributes to induction of chemokine and proinflammtory cytokine expressions in A549 alveolar epithelial cells2007

    • Author(s)
      Juthaporn Kohwiwattanagun, Ikuo Kawamura, Takao Fujimura, and Masao Mitsuyama
    • Journal Title

      Microbiol. Immunol 51(2)

      Pages: 253-261

    • Peer Reviewed
  • [Journal Article] Mycobacterium tuberculosis H37Rv is capable of inhibiting necrosis of the infected macrophages via activation of caspase-92007

    • Author(s)
      Ryosuke Uchiyama, Ikuo Kawamura, Michiko Kawanishi, Kohsuke Tsuchiya, Takanari Tominaga, Taijin Kaku, Yutaka Fukasawa, Shunsuke Sakai, Takao Fujimura, Takamasa Nomura and Masao Mitsuyama
    • Journal Title

      Infect. Immun 75(6)

      Pages: 2894-2902

    • Peer Reviewed
  • [Journal Article] Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria2007

    • Author(s)
      Nomura Takamasa, Ikuo Kawamura , Chikara Kohda , Hisashi Baba , Yutaka Ito , Terumi Kimoto , Isao Watanabe, and Masao Mitsuyama
    • Journal Title

      Microbiology 153(7)

      Pages: 2250-2258

    • Peer Reviewed
  • [Journal Article] Cytolysin-dependent endosomal escape is required but not sufficient for the induction of Th1 immune response against Listeria infection: A distinct role for listeriolysin O as determined by cytolysin gene replacement2007

    • Author(s)
      Hideki Hara, Ikuo Kawamura, Takamasa Nomura, Takanari Tominaga, and Masao Mitsuyama
    • Journal Title

      Infect Immun 75(8)

      Pages: 3791-3801

    • Peer Reviewed
  • [Journal Article] RD1 region in mycobacterial genome is involved in induction of necrosis in the infected RAW264 cells via mitochondrial membrane damage and ATP depletion2007

    • Author(s)
      Taijin Kaku, Ikuo Kawamura, Ryosuke Uchiyama, Takeshi Kurenuma, and Masao Mitsuyama
    • Journal Title

      FEMS icrobiology Letters 274(2)

      Pages: 189-195

    • Peer Reviewed
  • [Journal Article] Vaccine and drug development (a review) Tuberculosis2007

    • Author(s)
      Masao Mitsuyama and David N. McMurray: Tuberculosis
    • Journal Title

      87

      Pages: S10-S13

    • Peer Reviewed
  • [Journal Article] 抗結核防御免疫と結核菌による免疫制御2007

    • Author(s)
      河村伊久雄、光山正雄
    • Journal Title

      実験医学25(20)増刊 105(3183)-111(3189)

  • [Journal Article] 病原体の感染戦略の新知見化学療法の領域2007

    • Author(s)
      光山正雄
    • Journal Title

      結核菌の病原性発現機構はどこまでわかったか化学療法の領域 23(11)

      Pages: 1750-1756

    • Peer Reviewed
  • [Journal Article] Dependence of the lethal effect of pore-forming cytolysins on the cytolytic activity2006

    • Author(s)
      Isao Watanabe, Takamasa Nomura, Takanari Tominaga, Kazuhiro Yamamoto, Chikara Kohda, Ikuo Kawamura, and Masao Mitsuyama
    • Journal Title

      Journal of Medical Microbiology 55

      Pages: 505-510

    • Peer Reviewed
  • [Journal Article] Limited contribution of Toll-like receptor (TLR) 2 and TLR4 to the host response to a fungal infectious pathogen, Cryptococcus neoformans2006

    • Author(s)
      Kiwamu Nakamura, Kazuya Miyagi, Yoshinobu Koguchi, Yuki Kinjo, Kaori Uezu, Takeshi Kinjo, Morikazu Akamine, Jiro Fujita, Ikuo Kawamura, Masao Mitsuyama, Yoshiyuki Adachi, Naohito Ohno, Kiyoshi Takeda, Shizuo Akira, Akiko Miyazato, Mitsuo Kaku, and Kazuyoshi Kawakami
    • Journal Title

      FEMS Immunology and Medical Microbiology 47(1)

      Pages: 148-154

    • Peer Reviewed
  • [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands: Involvement of masct cells and dendritic cells2006

    • Author(s)
      Kazuhiro Yamamoto, Ikuo Kawamura, Juichi Ito, and Masao Mitsuyama
    • Journal Title

      Clinical and Experimental Allergy 36(6)

      Pages: 760-769

    • Peer Reviewed
  • [Journal Article] Listeriolysin 0 derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice2006

    • Author(s)
      Kazuhiro Yamamoto, Ikuo Kawamura, Takanari Tominaga, Takamasa Nomura, Junichi Ito, and Masao Mitsuyama
    • Journal Title

      Clinical and Experimental Immunology 144

      Pages: 475-484

    • Peer Reviewed
  • [Journal Article] Sustained-release vancomycin sheet may help to prevent prosthetic graft methicillin-resistant Staphylococcus aureus infection2006

    • Author(s)
      Hirose K, Marui A, Arai Y, Nomura T, Inoue S, Kaneda K, Kamitani T, Fujita M, Mitsuyama M, Tabata Y, and Komeda M
    • Journal Title

      J. Vasc. Surg 44

      Pages: 377-382

    • Peer Reviewed
  • [Journal Article] Involvement of Toll-like receptor 2 signaling in the phagocytosis of Listeria monocytogenes by murine macrophages

    • Author(s)
      Yanna Shen, Ikuo Kawamura*, Takamasa Nomura, Kohsuke Tsuchiya, Hideki Hara, Huixin Qu, Sita R. Dewamitta, Takeshi Yamamoto, Sylvia Daim, Shunsuke Sakai, Takeshi Kurenuma and Masao Mitsuyama
    • Journal Title

      Submitted to Cellular Microbiology

    • Peer Reviewed
  • [Journal Article] Listeriolysin O, a major virulence factor of Listeria monocytogenes, is a novel Toll-like receptor (TLR) agonist recognized by TLR2 and TLR4

    • Author(s)
      Ikuo Kawamura, Chikara Kohda, Kohsuke Tsuchiya, Yutaka Ito, Terumi Kimoto, Takamasa Nomura and Masao Mitsuyama
    • Journal Title

      submitted to J. Exp. Med. Submitted to J Immunol (in revision)

    • Peer Reviewed
  • [Presentation] 細胞内寄生性リステリア病原因子による宿主免疫応答の誘導機構-生菌免疫の作用機序へのアプローチ-2009

    • Author(s)
      光山正雄
    • Organizer
      第82回日本細菌学会
    • Place of Presentation
      名古屋市国際会議場
    • Year and Date
      2009-04-13
  • [Presentation] Mycobacterium tuberculosis, Listeria monocytogenes and the host cytokine response2008

    • Author(s)
      Masao Mitsuyama
    • Organizer
      The 11st Kyoto University International Symposium in Shanghai
    • Place of Presentation
      復旦大学上海医科大学ホール 中国
    • Year and Date
      2008-10-11
  • [Presentation] 細菌感染に対する免疫応答2008

    • Author(s)
      光山正雄
    • Organizer
      第38回日本耳鼻咽喉科感染症研究会
    • Place of Presentation
      島根県民会館ホール 松江市
    • Year and Date
      2008-09-05
  • [Presentation] Critical involvement of bacterial virulence factor in the host immune response to Listeria monocytogenes2008

    • Author(s)
      Masao Mitsuyama
    • Place of Presentation
      国際微生物学会議2008イスタンブール トルコ
    • Year and Date
      2008-08-08
  • [Presentation] 感染症を巡る最近の進歩2008

    • Author(s)
      光山正雄
    • Organizer
      第134回日本医学会シンポ
    • Place of Presentation
      日本医師会館大ホール 東京
    • Year and Date
      2008-07-17
  • [Presentation] 細胞内寄生性細菌リステリアの病原因子と宿主免疫応答2008

    • Author(s)
      光山正雄
    • Organizer
      日本乳酸菌学会2008年度年次大会
    • Place of Presentation
      京都大学百周年時計台記念館ホール
    • Year and Date
      2008-07-14
  • [Presentation] 細胞内寄生性細菌の細胞内生存戦略と感染宿主の免疫応答-細菌の病原因子と宿主防御機構のせめぎあい-2008

    • Author(s)
      光山正雄
    • Organizer
      第42回大分感染症研究会
    • Place of Presentation
      大分全日空ホテル
    • Year and Date
      2008-02-07
  • [Presentation] Mechanism in the requirement of listeriolysin O (LLO) for the induction of caspase-dependent cytokines in macrophages infected with Listeria monocytogenes2007

    • Author(s)
      Masao Mitsuyama
    • Organizer
      In Minisymposium 73- Mechanism of protection against intracellular pathogens, Symposium Chair and oral presentation at 13th International Congress of Immunology
    • Place of Presentation
      Rio de Janeiro, Brazil
    • Year and Date
      2007-08-25
  • [Presentation] Interaction of intracellular parasitic bacteria andhost defense system for the generationof Th1-dependent acquired immunity2007

    • Author(s)
      Masao Mitsuyama
    • Organizer
      COE International Symposium 2007
    • Place of Presentation
      Shiran-kaikan, Kyoto
    • Year and Date
      2007-06-02
  • [Presentation] 医学(系学)部における微生物学教育のレーゾン・デートル2007

    • Author(s)
      光山正雄
    • Organizer
      第80回日本細菌学会総会シンポジウムSS1 : 微生物学教育のあり方を考える
    • Place of Presentation
      大阪市 アジア太平洋トレードセンターATC ホール
    • Year and Date
      2007-03-28
  • [Book] In Handbook of Listeria monocytogenes Ed by Dongyou Liu2008

    • Author(s)
      Masao Mitsuyama
    • Total Pages
      540
    • Publisher
      Adaptive Immunity to Listeria monocytogenes
  • [Book] 生体防御医学事典2007

    • Author(s)
      光山正雄
    • Total Pages
      365
    • Publisher
      朝倉書店
  • [Book] 結核第1章A 結核菌の基礎(泉孝英監修 冨岡洋海編集)2006

    • Author(s)
      光山正雄(分担執筆)
    • Total Pages
      458(3-17)
    • Publisher
      医学書院(第1刷発行)
  • [Book] メルクマニュアル2006

    • Author(s)
      光山正雄
    • Total Pages
      1524-1575
    • Publisher
      日経BP 社
  • [Remarks]

    • URL

      http://bisei15.mb.med.kyoto-u.ac.jp/index.html

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Published: 2010-06-10   Modified: 2016-04-21  

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