2007 Fiscal Year Final Research Report Summary
Resistance of oppotunistic pathogens via enviromental respsonse systems and xenobiotics efflux systems to human innate immunosystems
| Project/Area Number |
18390137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
GOTOH Naomasa Kyoto Pharmaceutical University, 薬学部, Professor (30121560)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Jun Akita Prefectural University, 生物資源科学部, Associate Professor (00181256)
皆川 周 京都薬科大学, 薬学部, 助教 (50445962)
FUKUSHIMA Jun Kyoto Pharmaceutical University, Assitant Professor (00181256)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Pseudomonas aerurinosa / Serracia marcescens / pathogen / Seum resistance / quorum sensing / efflux system / complements / lipopolysaccharide |
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| Research Abstract |
Resistance to a human natural immunomechanism by Pseudomonas aeruginosa and Serracia marcescens that were the Gram-negative opportunistic bacteria, and the following result was obtained.
1) Pseudomonas aeruginosa: When the appearance of elastase synthesized gene (lasB) and Rhamunolipid synthesized gene (rhlA) with the acyl-homoserine lactones (HSLs) was compared, 3-oxo-C12-HSL was the highest. The deficiency of mexB has increased the appearance of lasB that responds to 3-oxo-Cn-HSL of C8 to C14 at a different rate. Each manifestation of a gene, the pyocyanine, and the change in the amount of the elastase of the production life were corresponding. The xenobiotic efflux system MexAB-OprM of P. aeruginosa not only took part in the multidrug-resistance but also selecting acyl-HSLs that had to respond, controlling quorum sensing system, and contributing to pathogenic expression to the human.
2) Serracia marcescens. Tn insertion mutant library of about 8,000 clones was constructed, and the serum susceptibility of all clones was examined. Then, 79 serum susceptible mutants were obtained. When Tn inserted genes in the these serum susceptible mutants was analyzed, it was shown that Tn insertion in genes encoding to core region of the lipopolysaccharide and concerning the synthesis of O antigen increased the serum susceptibility. It was suggested that the conformational change on the cell surface plays an important role to the serum resistance of S. marcescens against human complements.
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[Journal Article] Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan.2007
Author(s)
Ohara, M., Kouda, S., Onodera, M., Fujiue, Y., Sasaki, M., Kohara, T., Kashiyama, S., Hayashida, S., Kadono, M., Komatsuzawa, H., Gotoh, N., Usui, T., Itaha, H., Kuwabara, M., Yokoyama, T., Sugai, M.
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Journal Title
Microbiol. Immunol. 51
Pages: 271-277
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Quorum sensing system lactones do not increase invasiveness of a MexAB-OprM efflux mutant but do play a partial role in Pseudomonas aeruginosa invasion.2006
Author(s)
Kondo, A., Hirakata, Y., Gotoh, N., Fukushima, K., Yanagihara, K., Ohno, H., Higashiyama, Y., Miyazaki, Y., Nishide, K., Node, M., Yamada, Y, Kohno, S., Kamihira, S.
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Journal Title
Microbiol. Immunol 50
Pages: 395-401
Description
「研究成果報告書概要(欧文)」より
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