2009 Fiscal Year Final Research Report
Development of specific immunoglobulin against environmental sensitizing chemicals and actual survey on sensitizing status
| Project/Area Number |
18390187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
KAWAMOTO Toshihiro University of Occupational and Environmental Health, Japan, 医学部, 教授 (60177748)
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| Co-Investigator(Kenkyū-buntansha) |
OYAMA Tsunehiro 産業医科大学, 医学部, 准教授 (00309965)
ISSE Toyohi 産業医科大学, 医学部, 准教授 (80341494)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 環境 / 感作 / 化学物質 / アレルギー / 免疫グロブリン / アルデヒド脱水素酵素 / ノックアウトマウス / 共有結合 |
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| Research Abstract |
Structural changes of our body proteins caused by the binding of chemical substances are one of the most important mechanisms of allergy onset. When human serum albumin (HSA) was mixed with sensitizing chemical substances, the fluorescence (280nm excitation and 340nm emission) of HSA decreased dose-dependently. The reduction of HSA fluorescence by treatment with chemicals was by the following order ; commonly designated sensitizers> possible sensitizers> non-sensitizers. HSA fluorescence after treatment with test chemicals may be a good predictor for their sensitizing potentials. The antigenicity of chemical-HSA adducts toward sera from sensitized patients was varied according to the condition in which the adducts were formed. An effective method to detect antibody against chemical-HSA adducts were developed. Acetaldehyde inhalation caused more DNA adducts at upper respiratory tracts in Aldh2 knockout mice more than in wild type mice.
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[Journal Article] Cholinergic alterations following alcohol exposure in the frontal cortex of Aldh2-deficinet mice models.2009
Author(s)
Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K-I, Ijiri I, Kinoshita H
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Journal Title
Brain Research 1295
Pages: 37-43
Peer Reviewed
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[Journal Article] ALDH-2 deficiency increases vascular oxidative stress-Evidence for indirect antioxidative properties.2008
Author(s)
Wenzel P, Muller J, Zurmeyer S, Schumacher S, Schulz E, Oelze M, Pautz A, Kawamoto T, Wojnowski L, Kleinert H, Munzel T.
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Journal Title
Biochemical and Biophysical Research Communications 367
Pages: 137-143
Peer Reviewed
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[Journal Article] Increased formation of hepatic N2-ethylidene-2'-deoxyguanosine DNA adduct in aldehyde dehydrogenase 2 knockout mice treated with ethanol.2007
Author(s)
Matsuda T, Matsumoto A, Uchida M, Kanaly R, Misaki K, Shinya Shibutani S, Kawamoto T, Kitagawa K, Nakayama K-I, Tomokuni K, Ichiba M
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Journal Title
Carcinogenesis 28(11)
Pages: 2363-2366
Peer Reviewed
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[Book] Enzymology and Molecular Biology of Carbonyl Metabolism-12.(Weiner, H., Plapp, B., Lindahl, R., Maser, E. (Eds), Actalodehyde elimination changes in transgenic mice lacking aldehyde dehydrogenase 2 activity.)2006
Author(s)
Isse T, Oyama T, Matsuno K, Kitagawa K, Ogawa M, Kinaga T, Suzuki-Narai R, Yamaguchi T, Uchiyama I, Kawamoto T
Total Pages
33-41
Publisher
Purdue University Press
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