2007 Fiscal Year Final Research Report Summary
Production of interferon stimulated gene library and screening of new therapeutic drug for hepatitis viruses
| Project/Area Number |
18390218
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Hiroshima University |
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Principal Investigator |
CHAYAMA Kazuaki Hiroshima University, Graduate School of Biomedical Sciences, Professor (00211376)
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| Project Period (FY) |
2006 – 2007
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| Keywords | interferon / hepatitis C virus / interferon stimulated genes / expression profile / microarray / mRNA / human hepatocyte chimeric mouse / therapeutic drug |
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| Research Abstract |
We inflicted human hepatocyte chimerie mice with hepatitis B and hepatitis C viruses by injecting serum samples obtained from serum samples of chronic hepatitis B and hepatitis C virus carriers. After confirming the development of viremia in mice, we administered daily 7000 U/g of alpha interferon intramusculary. A rapid decrease in hepatitis C virus RNA levels was observed in all treated mice In contrast, only faint reduction of hepatitis B virus DNA was observed in mice infected with hepatitis B virus. We also administered the same amount of interferon to some human hepatocyte chimeric mice. We sacrificed and collected liver samples from each mice and extracted mRNA from each maim. Using them mRNA samples we performed microarray analysis. We obtained expression profiles from control, non-infected and non-interferon treated mice. We compared expression profiles of mice liver obtained from hepatitis B virus and hepatitis C virus infected mouse as well as interferon treated mice inflicted with hepatitis B and C viruses and treated with interferon. Up regulation of interferon stimulated genes ware observed in hepatitis C virus infected mice The up regulation of them genes was not so eminent in mice infected with hepatitis B virus. Interestingly, up regulation of some interferon stimulated genes were diminished in mice infected with hepatitis C virus compared with non-infected mouse. Further research is now in progress to analyze a mechanism underlining this suppression of interferon stimulated gene production.
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[Journal Article] Eicosapentaenoic acid could permit maintenance of the original ribavirin dose in chronic hepatitis C virus patients during the first 12 weeks of combination therapy with pegylated interferon-alpha and ribavirin. A prospective randomized controlled trial2007
Author(s)
Takaki, S., Kawakami, Y., Imamura, M., Aikata, H., Takahashi, S., Ishihara, H., Tsuji, Chayama, K., et. al.
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Journal Title
Intervirology 50(6)
Pages: 439-46
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Pretreatment predictor of response, time to progression, and survival to intraarterial 5-fluorouracil/ interferon combination therapy in patients with advanced hepatocellular carcinoma2007
Author(s)
Uka, K., Aikata, H., Takaki, S., Miki, D., Kawaoka, T., Jeong, S.C., Chayama, K., et. al.
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Journal Title
J Gastroenterol 42(10)
Pages: 845-53
Description
「研究成果報告書概要(欧文)」より
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