2007 Fiscal Year Final Research Report Summary
Identification of molecular target genes for therapy in hepatocellular carcinoma.
| Project/Area Number |
18390223
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YASUI Kohichiroh Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (30323695)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hepatocelluar carcinoma / genome / gene amplification / deletion |
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| Research Abstract |
High-density single nucleotide polymorphism (SNP) array analysis revealed novel amplification at 1q21 in cell lines derived from hepatocellular carcinomas (HCCs). Fluorescence in situ hybridization and real-time quantitative polymerase chain reaction (PCR) studies verified amplification at 1q21. An increase in copy-number at the region was detected in 32 (89%) of the 36 primary HCC tumors. To identify the targets for amplification, we examined 19 HCC cell lines for expression levels of all 26 genes located within the 700-kb amplified region. Five genes were over-expressed in cell lines with amplification at 1q21. Among these, CREB3L4 (cyclic AMP responsive element binding protein 3-like 4), INTS3 (integrator complex subunit 3) and SNAPAP(snare-associated protein snapin)were significantly over-expressed in tumors from 18 HCC patients when compared with counterpart nontumorous tissues. Our findings suggest that CREB3L4, INTS3 and SNAPAP are probable targets for the amplification mechanism and may therefore be involved, together or separately, in the development and/or progression of HCCs.
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[Journal Article] CREB3L4, INTS3, and SNAPAP are targets for the 1q21 amplicon frequently detected in hepatocellular carcinoma2008
Author(s)
Inagaki, Y, Yasui, K, Endo, M, Nakajima, T, Zen K, Tsuji, K, Minami, M, Tanaka, S, Taniwaki, M, Itoh, Y, Arii, S, Okanoue, T
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Journal Title
Cancer Genet Cytogenet 180
Pages: 30-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Guidelines for the antiviral therapy of hepatitis C virus carriers with normal serum aminotransferase based on platelet counts. 2008;382008
Author(s)
Okanoue, T, Itoh, Y, Minami, M, Hashimoto, H, Yasui, K, Yotsuyanagi, H, Takehara, T, Kumada, T, Tanaka, E, Nishiguchi, S, Izumi, N, Sata, M, Onji, M, Yamada, G, Okita, K, Kumada, H
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Journal Title
Hepatol Res 38
Pages: 27-36
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evidence of oxidative stress as a cofactor in the development of insulin resistance in patients with chronic hepatitis C. Hepatol Res2008
Author(s)
Mitsuyoshi, H, Itoh, Y, Sumida, Y, Minami, M, Yasui, K, Nakashima, T, Okanoue, T
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Journal Title
Hepatol Res 38
Pages: 348-353
Description
「研究成果報告書概要(欧文)」より
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[Presentation] MAPK7Identified as a Probable Target for Amplification at 17p11 in Hepatocellular Carcinoma2007
Author(s)
Keika, Zen, Kohichiroh, Yasui, Tomoaki, Nakajima, Yoshikazu, Inagaki, Shoji, Mitsufuji, Shinji, Tanaka, Masafumi, Taniwaki, Yoshito, Itoh, Shigeki, Arii, Takeshi, Okanoue
Organizer
The liver meeting 2007 (AASLD)
Place of Presentation
Boston, USA
Year and Date
20071102-06
Description
「研究成果報告書概要(欧文)」より
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