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2007 Fiscal Year Final Research Report Summary

Host defense system in the lung using pulmonary surfactant proteins and Toll-like reosptors

Research Project

Project/Area Number 18390241
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionSapporo Medical University

Principal Investigator

KUROKI Yoshio  Sapporo Medical University, School of Medicine, Professor (70161784)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroki  Sapporo Medical University, School of Medicine, Professor (60231396)
TAECAHASHI Motoko  Sapporo Medical University, School of Medicine, Associate Professor (00303941)
SHIMIZU Takeyuki  Sapporo Medical University, School of Medicine, Assistant Professor (10339137)
MITSUZAWA Hiroaki  Sapporo Medical University, School of Medicine, Research Associate (40325874)
NISHITANI Chiaki  Sapporo Medical University, School of Medicine, Research Associate (30381255)
Project Period (FY) 2006 – 2007
Keywordspulmonary surfactant / collectin / SP-A / SP-D / Toll-like receptor / pulmonary inflammation / endotoxin / MD-2
Research Abstract

The purposes of this project were to investigate the mechanisms by which pulmonary collections, surfactant proteins A and D (SP-A and SP-D), and Toll-like receptor (TISO function against infection and inflammation, and to establish the molecular basis for the clinical application of these proteins. The research results are summarized below.
1. We have found that SP-A and SP-D bind to TLR2, TLR4 and MD-2. SP-A inhibited the binding of smooth LPS on the cells expressing TLR4 and MD-2 and suppresses inflammation elicited by smooth LPS. The functional domain was the carbohydrate recognition domain (CRD) of SP-A, but the collagenase-resistant fragment of SP-A (CRF) in which the collagenous domain was removed bound to TLR4 very weakly and did not inhibit LPS-induced inflammation, indicating that the oligometric structure composed of SP-A octadecamers is important for expressing its function. Unlike SP-A SP-D inhibits inflammation elicited by different serotypes of LPS, rough LPS and smooth LPS. The experiments with SP-A/SP-D chimeric proteins revealed that the cruciform structure of SP-D composed of long collagenous domain was crucial for suppressing inflammation.
2. We have also found that the amino-terminal region of TLR4, Glu24-Lys47, is a site for MD-2 binding. Recombinant soluble forms of the extracellular TLR4 domain and MD-2 were demonstrated to dampen endotoxin-induced pulmonary inflammation in mice.
3. The experiments with TLR4 mutant, TLR4C88A, revealed that MD-2 possesses crucial abilities to express cell surface expression of TLR4 and its N-linked complex type glyoosylation.
4. SP-A and SP-D bound to Mycobacterium avium and induced bacrerial aggregation. SP-D possesses a potent ability to aggregate M. avium compared with SP-A The ligand for SP-D on M. avium was lipoarabinomannan (LAM) and that for SP-Aexisted in the lipid fraction extracted M. avium.

  • Research Products

    (58 results)

All 2008 2007 2006

All Journal Article (29 results) (of which Peer Reviewed: 12 results) Presentation (28 results) Book (1 results)

  • [Journal Article] 肺サーファクタント蛋白質(SP・A、SP-D)の免疫調節機能2008

    • Author(s)
      黒木 由夫
    • Journal Title

      臨床検査 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A2007

    • Author(s)
      Sano H
    • Journal Title

      J Biol Chem 282

      Pages: 7973-7981

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] SP-Dは糖鎖認識領域を介してTLR2およびTLR4に結合する2007

    • Author(s)
      西谷 千明
    • Journal Title

      分子呼吸器病 11

      Pages: 53-55

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 肺コレクチンによる自然免疫機構-臨床との接点2007

    • Author(s)
      黒木 由夫
    • Journal Title

      The Lung 15

      Pages: 41-46

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pulmonary collectins in innate immunity of the lung2007

    • Author(s)
      Kuroki Y
    • Journal Title

      Cell Micmbiol 9

      Pages: 1871-1879

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Comparative sequence analysis of leucine-rich repeats(LRRs)within vertebrate toll-like receptors2007

    • Author(s)
      Matsushima N
    • Journal Title

      BMC Genomics 8

      Pages: 124-143

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Autoantibody to alanyl-tRNA synthetase in patients with idiopathic pulmonary fibrosis2007

    • Author(s)
      Takahashi T
    • Journal Title

      Respirology 12

      Pages: 642-653

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Pulmonary collectins in innate immunity of the lung2007

    • Author(s)
      Kuroki, Y
    • Journal Title

      CeU Microbiol 9

      Pages: 1871-1879

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors2007

    • Author(s)
      Matsushima, N
    • Journal Title

      BMC Genomics 8

      Pages: 124-143

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Autoantibody to alanyl-tRNA synthetase in patients with idiop athic pulmonary fibrosis2007

    • Author(s)
      Takahashi, T
    • Journal Title

      Respirology 12

      Pages: 642-653

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunomodulatory functions of pulmonary collectins2007

    • Author(s)
      Mitsuzawa, H
    • Journal Title

      In Collagen-Related Lectins in Innate Immunity (Edited by Dave Kirkpatrick) 237

      Pages: 179-190

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Alloiococcus otitidis is a ligand for collectins and Toll-like receptor 2,and its phagocytosis is enhanced by collectins2006

    • Author(s)
      Konishi M
    • Journal Title

      Eur J Immunol 36

      Pages: 1527-1536

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Human pulmonary surfactant protein D binds the extracellular domain of Toll-like receptors 2 and 4 through the carbohydrate recognition domain by a mech-anism different from its binding to phosphatidylinositol and lipopolysaccharide2006

    • Author(s)
      Ohya M
    • Journal Title

      Biochemistry 45

      Pages: 8657-8664

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Surfactant protein A directly interacts with TLR4 and N-D-2,and regulates inflammatory cellular response:importance of supratrimeric oligomerization2006

    • Author(s)
      Yamada C
    • Journal Title

      J Biol Chem 281

      Pages: 21771-21780

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Mannose-binding lectin augments the uptake of lipid A,and Staphylococcus aureus,and Fschericia coli by Kupffer cells through increased cell surface expression of scavenger receptor A2006

    • Author(s)
      Ono K
    • Journal Title

      J Immunol 177

      Pages: 5517-5523

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Recombinant soluble forms of extracellular Toll-like receptor 4 domain and MD-2 inhibit LPS binding on cell surface and dampen LPS-induced pulmonary inflammation mice2006

    • Author(s)
      Mitsuzawa H
    • Journal Title

      J Immunol 177

      Pages: 8133-8139

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Surfactant protein A without the interruption of Gly-X-Y repeats loses a kink of oligomeric structure and exhibits impaired phospholipid liposome aggregation ability2006

    • Author(s)
      Uemura T
    • Journal Title

      Biochemishy 45

      Pages: 14543-14551

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Toll-like receptor 4 region Glu24-Lys47 is a site MD-2 binding: importance of Cys29 and Cys402006

    • Author(s)
      Nishitani C
    • Journal Title

      J Biol Chem 281

      Pages: 38322-38329

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] コレクチンを用いたDDSの可能性をみる2006

    • Author(s)
      黒木 由夫
    • Journal Title

      分子呼吸器病 10

      Pages: 50-53

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Crfluoro-8-methoxy-quinolone,gatifloxacin,down-regulates Il-8 production in rostate cell line PC-32006

    • Author(s)
      Takeyama K
    • Journal Title

      Antimicrobial Agents and Chemotherapy 51

      Pages: 162-168

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Alloiococcus otitidis is a ligand for collectins and Toll-like receptor 2, and its phagocytosis is enhanced by collectins.2006

    • Author(s)
      Konishi, M
    • Journal Title

      Eur J Immunol 36

      Pages: 1527-1536

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human pulmonary surfactant protein D binds the extracellular domain of Toll-like receptors 2 and 4 through the carbohydrate recognition domain by a mechanism different from its binding to phosphatydulinositol and lipopolysaccharide2006

    • Author(s)
      Ohya, M
    • Journal Title

      Biochemistry 45

      Pages: 8657-8664

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surfactant protein A interacts with TLR4 and MD-2, and regulates inflammatory cellular response : importance of suprametric ologomerization2006

    • Author(s)
      Yamada, C
    • Journal Title

      J Biol Chem 281

      Pages: 21771-21780

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mannose-binding lectin augments the uptake of lipid A, Staphylococcus aureus, and Escherichia coli by Kupffer cells through, increased cell surface expression of scavenger receptor A2006

    • Author(s)
      Ono, K
    • Journal Title

      J Immunol 177

      Pages: 8133-8139

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surfactant protein A without the interruption of Gly-X-Y repeats loses a kink of oligomeric structure and exhibits impaired liposome aggregation.ability2006

    • Author(s)
      Uemura, T
    • Journal Title

      Biochemistry 45

      Pages: 14543-14551

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recombinant soluble forms of extracellular Toll-like receptor 4 domain and MD-2 inhibits LPS binding on cell surface and dampen LPS-induced pulmonary inflammation in mice2006

    • Author(s)
      Mitsuzawa, H
    • Journal Title

      J Immunol 177

      Pages: 8133-8139

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Toll-like receptor 4 region Glu24-Lys47 is a site for MD-2 binding : importance of Cys29 and Cys402006

    • Author(s)
      Nishitani, C
    • Journal Title

      J Biol Chem 281

      Pages: 38322-38329

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The 6-fluoro-8-methoxy-quinolone, gatiflbxacin, down-regulates IL-8 production in prostate cell line PC-32006

    • Author(s)
      Takeyama, K
    • Journal Title

      Anti-microbial Agents and Chemotherapy 51

      Pages: 162-168

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A2006

    • Author(s)
      Sano, H
    • Journal Title

      J Biol Chem 282

      Pages: 7973-7981

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The importance of the kink region of surfactant protein A in oligomer formation and lipid interaction2008

    • Author(s)
      Uemura T
    • Organizer
      第79回日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      20080600
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Pulmonary collecrins bind Legionella pneumophila2007

    • Author(s)
      Sawada K
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Pulmonary collectins bind Legionella pneumophila2007

    • Author(s)
      Sawada, K
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Human pulmonary surfactant protein D binds to MD-22007

    • Author(s)
      Nie X
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Pulmonary surfactant protein D suppresses inflammatory cellular responses stimulated with lipopolysaccharide in alveolar macrophages2007

    • Author(s)
      Yamazoe M
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Interaction of LRR-containing proteins with ligands2007

    • Author(s)
      Mikami T
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Toll-like receptor 4 region Glu24-Lys47 is a site for MD-2 binding2007

    • Author(s)
      Nislutani C
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Lung collectins and mannose binding lectin interact with Toll-like receptors by different mechanisms2007

    • Author(s)
      Shimizu T
    • Organizer
      第80回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Human pulmonary surfactant protein D binds to MD-22007

    • Author(s)
      Nie, X
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Pulmonary surfactant protein D suppresses inflammatory cellular responses stimulated with lipolysaccharide in alveolar macrophages2007

    • Author(s)
      Yamazoe, M
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Interaction of LRR-containing proteins with ligands2007

    • Author(s)
      Mikami, T
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Toll-like receptor 4 region Glu24-Lys47 is a site for MD-2 binding2007

    • Author(s)
      Nishitani, C
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Lung collectins and mannose binding lectin interact with Toll-like receptors by different mechanism2007

    • Author(s)
      Shimizu, T
    • Organizer
      The 80th Japan Biochemistry Society Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Pulmonary collectins in innate immunity of the lung2007

    • Author(s)
      Kurold Y
    • Organizer
      The 20th Naito Conference
    • Place of Presentation
      葉山(神奈川)
    • Year and Date
      2007-10-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Pulmonary collectins in innate immunity of the lung2007

    • Author(s)
      Kuroki, Y
    • Organizer
      The 20th Naito Conference
    • Place of Presentation
      Hayama, Kanagawa
    • Year and Date
      2007-10-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Surfactant protein directly interacts with TLR4 and MD-2 and regulates inflammatory cellular reponse2007

    • Author(s)
      Nishitani C
    • Organizer
      第7回あわじしま感染症・免疫フォーラム
    • Place of Presentation
      淡路島(兵庫)
    • Year and Date
      2007-09-02
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Surfactant protein directly interacts with TLR4 and MD-2 and regulates inflammatory cellular response.2007

    • Author(s)
      Nishitani, C
    • Organizer
      The 7th Awaji International Conference on Infection and Immunity
    • Place of Presentation
      Awaji Island, Hyogo
    • Year and Date
      2007-09-02
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Anionic surfactant pliospholipids inhibit inflammation from alveolar macrophages and U937 cells2007

    • Author(s)
      Kumnuma K
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      San Francisco,USA
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Anionic surfactant phospholipids inhibit inflammation from alveolar macrophages and U937 cells2007

    • Author(s)
      Kuronuma, K
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cys^<29> and Cys^<40> within the amino-terminal toll-like receptor 4 region are critical for interaction with MD-22006

    • Author(s)
      Nishitani C
    • Organizer
      第79回日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The importance of the kink region of surfactant protein A in oligomer formation and lipid interaction2006

    • Author(s)
      Uemura, T
    • Organizer
      The 79th Japan Biochemistry Society Meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cys29 and Cys40 within the amino-terminal toll-like receptor 4 region are critical for interaction with MD-22006

    • Author(s)
      Nishitani, C
    • Organizer
      The 79th Japan Biochemistry Society Meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Collectins,innate immunity and inflammation2006

    • Author(s)
      Kuroki Y
    • Organizer
      North American Cystic Fibrosis Meeting Symposium
    • Place of Presentation
      Denver,USA
    • Year and Date
      2006-11-05
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Collectins, innate inmmunity and inflammation2006

    • Author(s)
      Kuroki, Y
    • Organizer
      North American Cystic Fibrosis Meeting Symposium
    • Place of Presentation
      Denver, USA
    • Year and Date
      2006-11-05
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Surfactant protein A(SP-A)and D(SP-D)directly bind to the extracellular domains of TLR2 and TLR4 through the carbohydrate recognition domain2006

    • Author(s)
      Nishitani C
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Snowmass,USA
    • Year and Date
      2006-08-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Recombinant soluble forms of extraellular Toll-like receptor 4 domain and MD-2 inhibit LPS binding on cell surface and dampen LPS-induced pulmonary inflammation in mice2006

    • Author(s)
      Mitsuzawa H
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Snowmass,USA
    • Year and Date
      2006-08-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Surfactant proteins A (SP-A) and D (SP-D) directly bind to the extracellular domains of TLR2 and TLR4 through the carbohydrate recognition domain.2006

    • Author(s)
      Nishitani, C
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Snowmass, USA
    • Year and Date
      2006-08-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Recombinant soluble forms of extracellular Toll-like receptor 4 domain and MD-2 inhibit LPS binding on cell surface and dampen LPS-induced pulmonary inflammation in mice2006

    • Author(s)
      Mitsuzawa, H
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Snowmass, USA
    • Year and Date
      2006-08-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Immunomodulatory funcfions of pulmonary collectins In Collagen-Related Innate Immunity(Edited by Dave Kirkpatrik)2007

    • Author(s)
      Mitsuzawa H
    • Total Pages
      237(p179-190)
    • Publisher
      Reseazch Signpost
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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