Development and clinical application of cDNA microarray using formalin fixed paraffin embedded specimen

2009 Fiscal Year Final Research Report

• Project/Area Number: 18390362
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: UETAKA Hiroyuki Tokyo Medical and Dental University, 医歯学総合研究科, 寄附講座教員 (60311651)
• Co-Investigator(Kenkyū-buntansha): SUGIHARA Kenichi 東京医科歯科大学, 医歯学総合研究科, 教授 (10171167) ; HIGUCHI Teturo 東京医科歯科大学, 医歯学総合研究科, 助教 (90334416) ; IIDA Satoshi 東京医科歯科大学, 医歯学総合研究科, 助教 (20361694) ; ISHIKAWA Tosiaki 東京医科歯科大学, 医歯学総合研究科, 寄附講座教員 (00422478)
• Project Period (FY): 2006 – 2009
• Keywords: 大腸小腸肛門外科学

Research Abstract

Microarray technology is potentially a powerful approach to identify genes expression signatures that predict the effects of chemotherapeutic drugs. The aim of this study is to develop cDNA microarray using RNA isolated from laser-capture microdisssected formalin fixed paraffin embedded (FFPE) specimens.

Research Products

• [Journal Article] Over-and under-expressed microRNAs in human colorectal cancer (2009)
  • Author(s): Motoyama K, Inoue H, Takatsuno Y, Tanaka F, Mimori K, Uetake H, Sugihara K, Mori M.
  • Journal Title: Int J Oncol 34 Pages: 1069-1075
• [Journal Article] 【大腸癌治療の現在化学療法と外科手術のコンビネーション】病期 (ステージ) と大腸癌術後補助化学療法の適応 (2009)
  • Author(s): 植竹宏之, 杉原健一
  • Journal Title: Pharma Medica 27 Pages: 11-14
• [Journal Article] 【大腸がん化学療法のNEW STRATEGY】切除不能進行・再発大腸がんに対する化学療法 大腸がん化学療法におけるベバシズマブの位置付けとその効果 (2009)
  • Author(s): 植竹宏之, 石川敏昭, 杉原健一
  • Journal Title: Mebio Pages: 66-71
• [Journal Article] Efficacy of laser capture microdissection plus RT-PCR technique in analyzing gene expression levels in human gastric cancer and colon cancer (2008)
  • Author(s): Makino H, Uetake H, Danenberg K, Danenberg PV, Sugihara K.
  • Journal Title: BMC Cancer 8 Pages: 210
• [Journal Article] Lactate dehydrogenase-5 (LDH-5) expression in human gastric cancer : Association with hypoxia-inducible factor (HIF-1 alpha) pathway, angiogenic factors production and poor prognosis (2008)
  • Author(s): Kolev Y, Uetake H, Takagi Y, Sugihara K.
  • Journal Title: Ann Surg Oncol 15 Pages: 2336-2344
• [Journal Article] Clinical Significance of High Mobility Group A2 in Human Gastric Cancer and Its Relationship to let-7 MicroRNA Family (2008)
  • Author(s): Motoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M.
  • Journal Title: Clin Cancer Res 14 Pages: 2334-2340
• [Journal Article] Messenger RNA expression of vascular endothelial growth factor and its receptors in primary colorectal cancer and corresponding liver metastasis (2008)
  • Author(s): Kobayashi H, Sugihara K, Uetake H, Higuchi T, Yasuno M, Enomoto M, Kuramochi H, Lenz HJ, Danenberg KD, Danenberg PV.
  • Journal Title: Ann Surg Oncol 15 Pages: 1232-1238
• [Journal Article] 大腸癌の発生から転移までのメカニズム (2007)
  • Author(s): 石川敏昭, 植竹宏之, 杉原健一
  • Journal Title: 薬局 58(5) Pages: 1855-1860
• [Journal Article] コンセンサス術後補助療法 大腸癌 (2007)
  • Author(s): 石川敏昭, 植竹宏之, 杉原健一
  • Journal Title: コンセンサス癌治療 6(2) Pages: 72-75
• [Journal Article] 下部消化管疾患における最近の話題 大腸癌の化学療法の進歩と限界 (2007)
  • Author(s): 植竹宏之, 石川敏昭, 杉原健一
  • Journal Title: 日本医師会雑誌 136(3) Pages: 535-538
• [Journal Article] 侵襲をめぐるQ&A血管新生 癌に対する血管新生阻害薬の臨床効果について教えて下さい (Q&A) (2007)
  • Author(s): 植竹宏之, 石川敏昭, 杉原健一
  • Journal Title: Surgery Frontier. 14(4) Pages: 463-465
• [Presentation] 進行再発大腸がん化学療法の新たな治療戦略 (2009)
  • Author(s): 植竹宏之
  • Organizer: 第20回日本消化器癌発生学会総会ランチョンセミナー
  • Place of Presentation: 広島
  • Year and Date: 2009-11-27
• [Presentation] 大腸癌肝転移に対する Bevacizumab 併用化学療法後肝切除の有用性 (2009)
  • Author(s): 植竹宏之, 石川敏昭, 光法雄介, 有井滋樹, 杉原健一
  • Organizer: 第17回 日本消化器関連学会週間
  • Place of Presentation: 京都
  • Year and Date: 2009-10-16
• [Presentation] Current status of chemotherapy for CRC in Japan (2009)
  • Author(s): Hiroyuki Uetake
  • Organizer: CURSO INTERNACIONAL DE ENDOSCOPIA DIAGNOSTICA Y TERAPEUTICA
  • Place of Presentation: Santiago, Chile
  • Year and Date: 2009-08-27
• [Presentation] Quantitative analysis of genes using FFPE sample was dependent to dilution of a formalin solution and soaking duration : A report of the SACURA Study Group (2009)
  • Author(s): Hiroyuki Uetake, Toshiaki Ishikawa, Hideki Ueno, Kenichi Sugihara, Naohiro Tomita
  • Organizer: The 101st annual meeting of AACR
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 2009-04-21
• [Presentation] Danenberg^1Molecular Signatures of Gastric Cancers Derived from Microarray Analysis of Paraffin-Embedded Specimens Predict the Degree of Response to CPT11/S-1 Chemotherapy (2008)
  • Author(s): Shinji Morita, Mikito Inokuchi, Hiroyuki Uetake, Andreas C. Hoffmann, Janine Cooc, Akiko Omori, Kenichi Sugihara, Kathleen D. Danenberg, Peter V.
  • Organizer: The annual meeting of ASCO
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2008-06-01