2007 Fiscal Year Final Research Report Summary
Clinical evaluation of efficacy and safety of dendritic cell-based vaccine therapy using with HLA-A2 restricted IL-13 alpha receptor peptide in patients with malignant glioma managed by international organization for standardization
| Project/Area Number |
18390395
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nagoya University |
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Principal Investigator |
WAKABAYASHI Toshihiko Nagoya University, University Hospital, Associate Professor (50220835)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Jun Nagoya University, Graduate School of Medicine, Professor (40158449)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Dendritic cell / Brain tumor / Molecular target / IL-13 / ISO / Cell therapy / CTL / Glioma |
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| Research Abstract |
As for dendritic cell therapy for malignant brain tumor using with specified antigen peptide, pre-clinical research had been evaluated until 2006 Since 2007, Clinical trial has started. with the support of the center for Genetic and Regenerative Medicine (CGRM) in our Hospital, which is an institute to put advanced medicine into practice in Nagoya University Hospital, gained ISO (International Organization for Standardization)9001 : 2000 and 13485 : 2003 Accreditation. It is the first academic institute in Japan to achieve the ISO Quality. Under this, it has been planed to work with clients in industry and academic centers in Japan, providing assistance in process development and validation, and GMP/GTP (Good Tissue Practice) production. There it has been responsible for process development, validation, and GMP (Good Manufacturing Practice) production of a broad range of novel cell including dendritic cell vaccines, antigen-specific CTLs (cytotoxic T lymphocytes).
In order to improve the prognosis of malignant glioma, dendritic cell therapy vaccinated with IL13R alpha 2 peptide, which reported to be abundantly and specifically overexpressed in glioma, has recently emerged. Patients positive for human leukocyte antigen (BLA) -A2 with histologically confirmed malignant glioma were enrolled. After accumulation of peripheral blood monocyte, CD14 positive cells were collected by Clini MACS. After one week incubation with cytokines (IL-4 and GM-CSF), matured DCs were pulsed by IL13R alpha 2 peptide, and patients were inoculated intradermally four times at 2-week intervals. Although there is small number of clinical trial (four patients), this DC therapy has shown safety; and patients were stable during treatment period. This study suggests the feasibility and safety of dendritic cell therapy vaccinated with IL13R alpha 2 peptide for clinical trial, which may become a new treatment options for patients with malignant gliomas.
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[Journal Article] The DNA demetbylating agent 5-aza-2-deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma2008
Author(s)
A. Natsume, T. Wakabayashi, K. Tsujimura, S. Shimato, M. Ito, K. Kuzushima, Y. Kondo, Y. Sekido, H. Kawatsura, Y. Narita, J. Yoshida
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Journal Title
International Journal of Cancer (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibition of Aurora-B function increases formation of multinucleated cell in p53 gene deficient cells and enhances anti-tumor effect of temozolomide in human glioma cells2007
Author(s)
T. Tsuno, A. Natsume, Katsumata, S.M. Mizuno, M. Fujita, H. Osawa, N. Nakahara, T. Wakabayashi, Y. Satoh, M. Inagaki, J. Yoshida
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Journal Title
J Neurooncol 83
Pages: 249-258
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intravenously transplanted human neural stem cells migrate Qto the injured spinal cord in adult mice in an SDF-1 and HGF-dependent manner2007
Author(s)
H. Takeuchi, A. Natsume, T. Wakabayashi, C. Aoshima, S. Shimato, M. Ito,J. Ishii, Y. Maeda, M. Hara, S.U. Kim, J. Yoshida
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Journal Title
Neurosci Lett 426
Pages: 69-74
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Brain metastases from apocrine carcinoma of the scalp : case report2006
Author(s)
S. Shimato, T. Wakabayashi, M. Mizuno, N. Nakahara, H. Hatano, A. Natsume, D. Ishii, Y. Hasegawa, I. Hyodo, T. Nagasaka, J. Yoshida
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Journal Title
J Neuro-OnceL 77(3)
Pages: 285-289
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] EGFR mutation in patients with brain metastases from lung cancer : correlation with the efficacy of gefitinib2006
Author(s)
S. Shimato, T. Mitsadomi, T. Kosaka, Y. Yatabe, T. Wakabayashi, M. Mizuno, N. Nakahara, H. Hatano, A. Natsume, D. Ishii, J. Yoshida
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Journal Title
Neuro-Oncol 8(2)
Pages: 137-144
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Human gene therapy for malignant gliomas2006
Author(s)
T. Wakabayashi, J. Yoshida, M. Mizuno, M. Fujii, Y. Kajita, N. Nakahara, H. Hatano
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Journal Title
Kanno T (Ed) Mimmally Invasive Neurosurgery and Multidisciplinary Neurotraumatology Springer-Verlag(Tokyo)
Pages: 119-123
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Neurosurgical procedure using the integrated system of intraoperative MRI and neuronavigation.(Invited speaker)2008
Author(s)
Toshihiko, Wakabayashi, Atsushi, Natsume, Masazumi, Fujii, Satoshi, Maezawa, Jun, Yoshida
Organizer
2008 Neurosurgery update in Korea University
Place of Presentation
Seonl, Korea
Year and Date
2008-03-01
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Interferon-beta gene therapy for high-grade glioma. (Invited speaker)2007
Author(s)
Toshihiko, Wakabayashi, Atsushi, Natsume, Masaaki, Mazuno, Masazumi, Fujii, Jun, Yoshida
Organizer
World federation of neurosurgical society, 13th Interim meeting/ the 12th Asian-Australasian congress of neurological surgeons
Place of Presentation
Nagoya, Japan
Year and Date
20071118-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Neurosurgical procedure using the integrated system of intraoperative MRI and neuronavigation(Luncheon semnmr : Invited speaker)2007
Author(s)
Toshihiko, Wakabayashi, Masazumi, Fujii, Yasukazu, Kajita, Satoshi, Maezawa, Jun, Yoshida
Organizer
World federation of neurosurgical society, 13th Interim meeting/ the 12th Asian-Australasian congress of neurological surgeons
Place of Presentation
Nagoya, Japan
Year and Date
20071118-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Design, development and manufacture of processed cells, tissues and gene products for clinical research at the center for genetic and regenerative medicine, Nagoya University Hospital.(Invited speaker)2007
Author(s)
Toshihiko, Wakabayashi, Atsushi, Natsume, Masazumi, Fujii, Masaaki, Mizuno, Jun, Yoshida
Organizer
13th Annual meeting of Japan Society of Gene Therapy
Place of Presentation
Nagoya, Japan
Year and Date
20070628-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Dendritic cell therapy vaccinated. with HLA-A2 restricted IL13R alpha 2 peptide in patients with malignant glioma : clinical trial and assessment of combination theranv with interferon-beta gene therapy2007
Author(s)
Toshihiko, Wakabayashi, Atsushi, Natsume, Masazumi, Fujii, Masaaki, Mizuno, Yasukazu, Kajita, Norimoto, Nakahara, Jun, Yoshida
Organizer
13th Annual meeting of Japan Society of Gene Therapy
Place of Presentation
Nagoya, Japan
Year and Date
20070628-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A diniral trial of cationic liposome conteining interferon-beta gene for patients with malignant glioma : autopsy and gene expression profiling2006
Author(s)
Toshihiko, Wakabayashi, Masaaki, Mizuno, Atsushi, Natsume, Masazumi, Fujii, Yasukazu, Kajita, Norimoto, Nakahnra, Jun, Yoshida
Organizer
12th Annual meeting of Japan Society of Gene Therapy
Place of Presentation
Tokyo, Japan
Year and Date
20060824-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 左側頭部腫瘍の一例2006
Author(s)
若林俊彦
Organizer
第21回東海脳腫瘍病理検討会
Place of Presentation
名古屋
Year and Date
2006-09-02
Description
「研究成果報告書概要(和文)」より
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[Presentation] A case of left temporal tumor2006
Author(s)
Toshihiko, Wakabayashi, Masazami, Fujii, Jun, Yoshida
Organizer
21th Tokai Brain Tumor Pathology Conference
Place of Presentation
Nagoya, Japan
Year and Date
2006-09-02
Description
「研究成果報告書概要(欧文)」より
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[Book] 家族性グリオーマ2006
Author(s)
若林俊彦
Total Pages
274
Publisher
シュプリンガーフェアラーク東京
Description
「研究成果報告書概要(和文)」より
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