Study on the treatment of dural arteriovenous fistula by the inhibition of angiogenic growth factors

2009 Fiscal Year Final Research Report

• Project/Area Number: 18390402
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: SAKAKI Toshisuke Nara Medical University, 医学部附属病院, その他 (20118029)
• Co-Investigator(Kenkyū-buntansha): NAKASE Hiroyuki 奈良県立医科大学, 医学部, 教授 (10217739)
• Project Period (FY): 2006 – 2009
• Keywords: 脳硬膜動静脈瘻 / 脳虚血 / 静脈梗塞 / 血管新生因子 / 遺伝子治療

Research Abstract

The precise mechanisms responsible for the development and growth of dural arteriovenous fistula (DAVF) remain unclear, but it has been hypothesized that vascular angiogenic growth factors might be involved in the pathogenesis. Also, it remains unknown that Brain-Derived Neurotrophic Factor (BDNF) effect on regional Cerebral Blood Flow (rCBF) and has neuroprotective effect in cerebral venous ischemia. The present study was undertaken to investigate whether intraventricular BDNF infusion effects on infarct size and suppression of apoptosis in cerebral venous ischemic lesion in a rat 2-vein occlusion (2-VO) model and effect on rCBF. As the results, there were no statistical differences in rCBF between BDNF-treated and control groups. It was found that the mean infarct volumes after 2-VO were significantly smaller in BDNF-treated animals than controls at 2 days (1.49±1.44% v.s.3.66±1.51%, P<0.05) and 7 days (0.93±0.47% v.s. 1.69±0.58%, P<0.05). The number of TUNEL positive cells detected at 2 days after 2-VO was significantly fewer in BDNF-treated animals (17.0±15.1) than controls (39.0±19.6, P<0.05). These results suggest that continuous intraventricular administration of BDNF protects apoptotic change of brain cortex and reduced infarct size without effect on rCBF in rat venous infarction model.

Research Products

• [Journal Article] Expression of angiogenic growth factor in the rat DAVF model. (2007)
  • Author(s): S hin Y, Nakase H, Nakamura M, Shimada K, Konishi N, Sakaki T
  • Journal Title: Neural Res 29(7) Pages: 727-33
• [Presentation] (2009)
  • Author(s): 堀内薫, 中瀬裕之, 田村健太郎, 竹島靖浩, 三宅仁, 榊寿右, LV Q., Heimann A., Li H., Kempski O.
  • Organizer: Stroke2009
  • Place of Presentation: 松江
  • Year and Date: 2009-03-22
• [Presentation] ペナンブラ領域でのシロスタゾール投与における梗塞巣及びアポトーシス発現に与える影響について (2008)
  • Author(s): 堀内薫, 三宅仁, 竹島靖浩, 乾登史孝, 田村健太郎, 平林秀裕, 中瀬裕之, 榊寿右
  • Organizer: 第20回日本脳循環代謝学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] ラット脳静脈虚血モデルを用いた脳虚血penumbraの基礎的研究～脳由来神経栄養因子の脳保護効果を中心に～ (2008)
  • Author(s): 竹島靖浩, 中瀬裕之, 木村僚太, 三宅仁, 堀内薫, 榊寿右
  • Organizer: 第20回日本脳循環代謝学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-06
• [Presentation] ラット脳静脈虚血モデルにおけるBDNFの神経保護作用について (2007)
  • Author(s): 竹島靖浩, 中瀬裕之, 西岡利和, 玉置亮, 乾登史孝, 三宅仁, 平林秀裕, 榊寿右
  • Organizer: 第19日本脳循環代謝会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2007-10-25
• [Presentation] ラット脳静脈虚血モデルにおけるBDNFの神経保護作用について (2007)
  • Author(s): 竹島靖浩, 中瀬裕之, 西岡利和, 田村健太郎, 玉置亮, 乾登史孝, 三宅仁, 堀内薫, 榊寿右
  • Organizer: 第66回日本脳神経外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-03
• [Presentation] ラット脳静脈虚血モデルにおけるBDNFの梗塞巣縮小効果 (2007)
  • Author(s): 竹島靖浩, 中瀬裕之, 西岡利和, 田村健太郎, 玉置亮, 乾登史孝, 三宅仁, 堀内薫, 榊寿右
  • Organizer: 第32回日本脳卒中学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2007-03-23
• [Presentation] (2007)
  • Author(s): 堀内薫, 西岡利和, 木村僚太, 田村健太郎, 玉置亮, 乾登史孝, 竹島靖浩, 三宅仁, 中瀬裕之, 榊寿右
  • Organizer: 第32回日本脳卒中学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2007-03-23