Molecular pathological approach to mechanism of cell invasion In pituitary adenoma

2008 Fiscal Year Final Research Report

• Project/Area Number: 18390405
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nippon Medical School
• Principal Investigator: TERAMOTO Akira Nippon Medical School, 大学院・医学研究科, 教授 (60231445)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Daizo 日本医科大学, 医学部, 准教授 (30210701) ; KIM Kyonsong 日本医科大学, 医科部, 助教 (30339387)
• Project Period (FY): 2006 – 2008
• Keywords: 下垂体腺腫 / 細胞浸潤 / 3-D invasion assay / RECK / SDF-1 / siRNA

Research Abstract

研究代表者による下垂体腺腫の手術標本と培養下垂体腺腫細胞を用いた。低酸素状態に陥った状態での研究を行った。その結果細胞表面に発現するRECK蛋白が低酸素刺激によりMMPへの刺激を介在し、その発現を促進させることで細胞浸潤を亢進させていることが明らかになった。さらに血管内皮細胞の前駆細胞を骨髄から呼び寄せる因子SDF-1も低酸素刺激によって産生され、浸潤傾向の強い下垂体腺腫ほど高く発現していることが明らかとなった。

Research Products

• [Journal Article] Trilostane-induced inhibition of cortisol secretion results in reduced negative feedback at the hypothalamic-pituitary axis (2009)
  • Author(s): Teshima T, Hara Y, Takekoshi S, Nezu Y, Harada Y, Yogo T, Teramoto A, Osamura RY, Tagawa M
  • Journal Title: Domest Anim Endocrinol 36(1) Pages: 32-44
• [Journal Article] MExpression of genes related to corticotropin production and glucocorticoid feedback in corticotroph adenomas of dogs with Cushing's disease (2009)
  • Author(s): Teshima T, Hara Y, Takekoshi S, Teramoto A, Osamura RY, Tagawa M
  • Journal Title: Domest Anim Endocrinol 36(1) Pages: 3-12
• [Journal Article] Transient uptake of thallium-201 into a cerebral infarction : a case report (2008)
  • Author(s): Morimoto D, Yoshida D, Noha M, Sasaki M, Takahashi H, Teramoto A
  • Journal Title: J Nippon Med Sch 75(6) Pages: 344-6
• [Journal Article] Expression of Wnt4 in human pituitary adenomas regulates activation of the beta-catenin-independent pathway (2008)
  • Author(s): Miyakoshi T, Takei M, Kajiya H, Egashira N, Takekoshi S, Teramoto A, Osamura RY
  • Journal Title: Endocr Pathol(Winter) 19(4) Pages: 261-73
• [Journal Article] Elevation of growth hormone-releasing hormone receptor messenger ribonucleic acid expression in growth hormone-secreting pituitary adenoma with Gsalpha protein mutation (2008)
  • Author(s): Sakai N, Kim K, Sanno N, Yoshida D, Teramoto A, Shibasaki T
  • Journal Title: Neurol Med Chir(Tokyo) 48(11) Pages: 481-7
• [Journal Article] Expansile neurenteric cyst arising in the frontal lobe associated with status epilepticus : report of a case and discussion of epileptogenesis (2008)
  • Author(s): Takumi I, Mori O, Mizutani N, Akimoto M, Kobayashi S, Teramoto A
  • Journal Title: Brain Tumor Pathol 25(2) Pages: 97-101
• [Journal Article] Prognosis of glioma patients bcombined immunostaining for survivin, Ki-67 and epidermal growth factor receptor (2008)
  • Author(s): Kogiku M, Ohsawa I, Matsumoto K, Sugisaki Y, Takahashi H, Teramoto A, Ohta S
  • Journal Title: J Clin Neurosci 15(11) Pages: 1198-203
• [Journal Article] Cushing's disease complicated with thrombosis in a dog (2008)
  • Author(s): Teshima T, Hara Y, Taoda T, Koyama H, Takahashi K, Nezu Y, Harada Y, Yogo T, Nishida K, Osamura RY, Teramoto A, Tagawa M
  • Journal Title: J Vet Med Sci 70(5) Pages: 487-91
• [Journal Article] Regulation of cell invasion and signalling pathways in the pituitary adenomaline, HP-75, by reversion-inducing cysteine-rich protein with kaza(RECK) (2008)
  • Author(s): Yoshida D, Nomura R, Teramoto A
  • Journal Title: J Neurooncol 89(2) Pages: 141-50
• [Journal Article] ACTH and alpha-Subunit are Co-expressed in Rare Human Pituitary Corticotroph CAdenomas Proposed to Originate fromACTH-Committed Early Pituitary Progenitor Cells (2008)
  • Author(s): Suzuki M, Egashira N, Kajiya H, Minematsu T, Takekoshi S, Tahara S, Sanno N, Teramoto A, Osamura RY
  • Journal Title: Endocr Pathol Pages: 29
• [Journal Article] Expression of pituitary tumour-derived, N-terminally truncated isoform of fibroblast growth factor receptor 4(ptd-FGFR4)correlates with tumour invasiveness but not with G-protein alphsubunit(gsp)mutation in human GH-secreting pituitary adenomas (2008)
  • Author(s): Morita K, Takano K, Yasufuku-Takano J, Yamada S, Teramoto A, Takei M, Osamura RY, Sano T, Fujita T.
  • Journal Title: Clin Endocrinol(Oxf) 68(3) Pages: 435-41
• [Journal Article] mmunohistochemical detection of somatostatin receptor(SSTR)subtypes 2A and 5 in pituitary adenoma from acromegalic patients : good correlation with preoperative response to octreotide (2007)
  • Author(s): Takei M, Suzuki M, Kajiya H, Ishii Y, Tahara S, Miyakoshi T, Egashira N, Takekoshi S, Sanno N, Teramoto A, Osamura RY
  • Journal Title: Endocr Pathol(Winter) 18(4) Pages: 208-16
• [Journal Article] Bilateral traumatic abducens nerve palsy associated with skull base fracture--case report (2007)
  • Author(s): Katsuno M, Yokota H, Yamamoto Y, Teramoto A
  • Journal Title: Neurol Med Chir(Tokyo) 47(7) Pages: 307-9
• [Journal Article] PTTG is a secretory protein in human pituitary adenomas and in mouse pituitary tumor cell lines (2007)
  • Author(s): Minematsu T, Egashira N, Kajiya HTakei M, Takekoshi S, Itoh Y, Tsukamoto H, Itoh J, Sanno N, Teramoto A, Osamura RY
  • Journal Title: Endocr Pathol 18(1) Pages: 8-15
• [Journal Article] The use of 3-D culture in peptide hydrogel for analysis of discoidin domain receptor 1-collagen interaction (2007)
  • Author(s): Yoshida D, Teramoto A
  • Journal Title: Cell Adh Migr 1(2) Pages: 92-8
• [Journal Article] Elevated cell invasion is induced by hypoxia in a human pituitary adenoma cell line (2007)
  • Author(s): Yoshida D, Teramoto A
  • Journal Title: Cell Adh Migr 1(1) Pages: 43-51
• [Journal Article] Enhancement of pituitary adenoma cell invasion and adhesion is mediated by discoidin domain receptor-1 (2007)
  • Author(s): Yoshida D, Teramoto A
  • Journal Title: J Neurooncol 82(1) Pages: 29-40
• [Journal Article] Analyses of the mechanism of intracellular transport and secretion of pituitary hormone, with an insight of the subcellular localization of pituitary hormone and its mRNA (2006)
  • Author(s): Matsuno A, Mizutani A, Takekoshi S, Itoh J, Okinaga H, Nishina Y, Takano K, Nagashima T, Osamura RY, Teramoto A
  • Journal Title: Brain Tumor Pathol 23(1) Pages: 1-5
• [Journal Article] Expression of laminin beta2 : a novel marker of hypoxia in pituitary adenomas (2006)
  • Author(s): Bao Y, Yoshida D, Morimoto D, Teramoto A
  • Journal Title: Endocr Pathol 17(3) Pages: 251-61
• [Journal Article] PTTG overexpression is correlated with angiogenesis in human pituitary adenomas (2006)
  • Author(s): Minematsu T, Suzuki M, Sanno N, Takekoshi S, Teramoto A, Osamura RY
  • Journal Title: Endocr Pathol 17(2) Pages: 143-53
• [Presentation] 下垂体腺腫におけるCXCR7発現の細胞内シグナル伝達における意義 (2008)
  • Author(s): 吉田大蔵、野村竜太郎、寺本明
  • Organizer: 第67回日本脳神経外科学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 20081000
• [Presentation] 下垂体腺腫におけるSDF-1発現の意義 (2008)
  • Author(s): 野村竜太郎、吉田大蔵、寺本明
  • Organizer: 第67回日本脳神経外科学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 20081000
• [Presentation] 下垂体腺腫におけるStemcell-derived factorとCXCR7を介したautocrine signaling (2008)
  • Author(s): 吉田大蔵、野村竜太郎、田原重志、石井雄道、寺本明
  • Organizer: 第18回日本間脳下垂体腫瘍学会
  • Place of Presentation: 福井
  • Year and Date: 20080000
• [Presentation] Signaling pathway bySDF-1/CXCR4 in pituitary adenoma (2008)
  • Author(s): Daizo Yoshida, Ryutaro Nomura, Akira Teramoto
  • Organizer: Annual Meeting of American Association of Neurological Surgeons
  • Place of Presentation: Chicago, USA
  • Year and Date: 2008-08-28
• [Presentation] 下垂体腺腫細胞のHP-75におけるRECKを介した細胞内シグナルの制御 (2007)
  • Author(s): 吉田大蔵、野村竜太郎、寺本明
  • Organizer: 第66回日本脳神経外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 20070000
• [Presentation] 下垂体腺腫細胞におけるRECKを介したsignal pathway (2007)
  • Author(s): 吉田大蔵、野村竜太郎、高橋弘、寺本明
  • Organizer: ウォーターフロントカンファレンス
  • Place of Presentation: 横浜
  • Year and Date: 20070000
• [Presentation] 下垂体腺腫細胞HP-75におけるRECKを介した細胞内シグナルの制御に関する研究 (2007)
  • Author(s): 吉田大蔵、野村竜太郎、寺本明
  • Organizer: 第39回日本臨床分子形態学会総会、第48回日本組織細胞学会総会合同学術集会
  • Place of Presentation: 甲府
  • Year and Date: 20070000
• [Presentation] Regulation of cell invasiand signalling pathways in the pituitary adenoma cell line, HP-75, reversion-inducing cysteine-rich protein with kazal motifs (RECK) (2007)
  • Author(s): Daizo Yoshida, Ryutaro Nomura, Akira Teramoto
  • Organizer: 2007 Congress of Neurosurgeons Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20070000
• [Presentation] ペプタイドハイドロゲルを用いた下垂体腺腫細胞の3D culture modelの意義 (2006)
  • Author(s): 吉田大蔵、森本大二郎、包義君、北川亮、寺本明
  • Organizer: 第16回日本間脳下垂体腫瘍学会
  • Place of Presentation: 神戸
  • Year and Date: 20060000
• [Presentation] Expression of β2 ; A Novel Marker of Hypoxia in Pituitary Adenomas (2006)
  • Author(s): Yijun Bao, Daizo Yoshida, Daijiro Morimoto, Yoshiyuki Osamura, , and Akira Teramoto
  • Organizer: 第16回日本間脳下垂体腫瘍学会
  • Place of Presentation: 神戸
  • Year and Date: 20060000
• [Presentation] 下垂体腺腫におけるDiscoidin Domain Receptor 1(DDR1)の腫瘍浸潤における生物学的意義に関する研究 (2006)
  • Author(s): 吉田大蔵、寺本明
  • Organizer: 第10回日本内分泌病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 20060000
• [Presentation] Cell Invasion and Adhesion of Pituitary Adenoma Cell Mediated by Discoidin Domain Receptor-1 to MatrMetalloproteinase-2 and -9 pathway (2006)
  • Author(s): Daizo Yoshida, Daijiro Morimoto Akira Teramoto
  • Organizer: 2006 AANS Annual Meeting
  • Place of Presentation: San Fransisco
  • Year and Date: 20060000
• [Presentation] 下垂体腺腫細胞HP-75のRECK発現の細胞浸潤における意義 (2006)
  • Author(s): 吉田大蔵、野村竜太郎、Hu shaoshan、寺本明
  • Organizer: 第17回日本間脳下垂体腫瘍学会
  • Place of Presentation: 山形
  • Year and Date: 20060000
• [Presentation] 下垂体腺腫におけるDiscoidin Domain Receptor 1(DDR1)の細胞浸潤に果たす意義に関する研究 (2006)
  • Author(s): 吉田大蔵, 森本大二郎, 包義君, 金景成, 饒波正博, 寺本明, 鈴木雅規, 長村義之
  • Organizer: 第37回日本臨床分子形態学会
  • Place of Presentation: 大阪
  • Year and Date: 20060000