2007 Fiscal Year Final Research Report Summary
Quantitative analysis in neurotrophic factor genes of spinal cord with complementary DNA microarray
| Project/Area Number |
18390411
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Fukui |
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Principal Investigator |
BABA Hisatoshi University of Fukui, Faculty of Medicine, Professor (00165060)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shigeru University of Fukui, Hospital, Associate Professor (80234821)
UCHIDA Kenzo University of Fukui, Hospital, Associate Professor (60273009)
FURUKAWA Shoei Gifu Pharmaceutical University, Faculty of pharmacy, Professor (90159129)
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| Project Period (FY) |
2006 – 2007
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| Keywords | spinal cord injury / gene transfer / regeneration / microarrav / neurotrophin |
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| Research Abstract |
Chronic mechanical compression of the spinal cord causes neural tissue damage, including loss of anterior horn cells around the level of injury. Exogenous delivery of neurotrophins to neuronal cells could provide neuroprotection to a spinal cord subjected to mechanical injury. We investigated the efficacy of retrograde gene delivery of adenoviral vector (AdV) carrying neurotrophin-3 (NT-3) gene into twy (twy/twy) mouse spinal cord anterior horn neurons with chronic and progressive mechanical compression at C1-C2 level. AdV-NT-3 was used for retrograde delivery via the sternomastoid muscle to the cervical spinal accessory motoneurons in 16-week-old adult twy mice with relatively mild spinal cord compression. Four weeks after the AdV-NT-3 or AdV-beta-galactosidase cDNA (LacZ) as a marker gene injection, the compressed cervical spinal cord was examined histologically, immunohistologically, and by immunoblot analysis. Immunoreactivity to NT-3 was significantly enhanced in the AdV-NT-3-injected twy mice compared with the AdV-LacZ-injected mice. The numbers of anterior horn neurons of Nissl-, choline acetyltransferase (ChAT)-, and trkC-stained and wheat germ agglutinin-horseradish peroxidase(WGA-HRP)-labeled neurons at the spinal cord level with maximum compression were significantly higher in AdV-NT-3-transfected than in AdV-LacZ-transfected twy mice. Retrograde NT-3 gene transfer to twy mouse anterior horn neurons increased neurite axonal length and arborization of WGA-HRP-labeled neurons. Our results suggest that targeted retrograde NT-3 gene delivery is feasible in the intact animal and that it enhances neuronal survival even under chronic mechanical compression of the spinal cord. (c) 2008Wiley-Liss, Inc.
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[Journal Article] Adenovirus-mediated retrograde transfer of neurotrophin-3 gene enhances survival of anterior horn neurons of twy/twy mice with chronic mechanical compression of the spinal cord2008
Author(s)
Uchida, K., Nakajima, H., Inukai, T., Takamura, T., Kobayashi, S., Furukawa, S., Baba, H
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Journal Title
J Neurosci Res 86(8)
Pages: 1789-1800
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Target muscles for retrograde gene delivery to specific spinal cord segments2008
Author(s)
Nakajima, H., Uchida, K., Kobayashi, S., Inukai, T., Yayama, T., Sato, R., Mwaka, E., Baba, H
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Journal Title
Neurosci Lett 435(1)
Pages: 1-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Rescue of rat anterior horn neurons ifter spinal cord injury by retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene2007
Author(s)
Nakajima, H., Uchida, K., Kobayashi, S., Inukai, T., Horiuchi, Y., Yayama, T., Sato, R., Baba, H
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Journal Title
J Neurotrauma 24(4)
Pages: 703-712
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Motor neuron involvement in experimental lumbar nerve root compression : a light and electron microsc opicstudy2007
Author(s)
Kobayashi, S., Uchida, K., Yayama, T., Takeno, K., Miyazaki, T., Shimada, S., Kubota, M., Nomura, E., Meir, A., Baba, H
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Journal Title
Spine 32(6)
Pages: 627-634
Description
「研究成果報告書概要(欧文)」より
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