2009 Fiscal Year Final Research Report
Analysis of ion channels associated with auditory sensation and construction of a model for hereditary hearing impairment
| Project/Area Number |
18390460
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
SHIMADA Shoichji Nagoya City University, 大学院・医学系研究科, 教授 (20216063)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Shingo 名古屋市立大学, 大学院・医学研究科, 教授 (80157750)
UGAWA Shinya 名古屋市立大学, 大学院・医学研究科, 教授 (20326135)
ISHIDA Yusuke 大阪大学, 大学院・医学系研究科, 講師 (30381809)
|
|---|
| Project Period (FY) |
2006 – 2009
|
| Keywords | 聴覚 / 内耳 / 有毛細胞 / イオンチャネル / ノックアウトマウス |
|---|
| Research Abstract |
We examined whether amiloride-sensitive proton-gated cation channels ASICs (acid-sensing ion channels) are expressed in the stereocilia of mouse cochlear hair cells. In situ hybridization studies revealed that ASIC1b was expressed in the inner and outer hair cells. Fluorescent immunohistochemistry and immunogold electron microscopic studies demonstrated that ASIC1b was located at the insertions of stereocilia into the hair cells. Our findings provide a novel molecular key to the understanding of cochkear physiology and pathophysiology.
|
