2007 Fiscal Year Final Research Report Summary
Craniofecial bone reganeration by mesenchymal stem cells of the craniofacial origin
| Project/Area Number |
18390478
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
HIRANO Akiyoshi Nagasaki University, Graduate School of Biomedical Sciences, Professor (90208835)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Mutsuhisa Nagasaki University, Graduate School of Engineering, Professor (20039689)
AKITA Sadanori Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (90315250)
NAGAYAMA Yuji Nagasaki University, Graduate School of Biomedical Sciences, Professor (30274632)
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| Project Period (FY) |
2006 – 2007
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| Keywords | transfection / human bone marrow-derived mesenchvmal stem cells / tissue regeneration and healing / congenital anomaly / life-style oriented diseases / bone maturation marker / vessel and nerve regeneration / facial bone growth |
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| Research Abstract |
Human bone marrow derived mesenchymal stem cell, hMSCs were tested for post-grafting kinetics in the craniofacial regions, human Green Fluorescent Protein (hGFP)CDNA was trasfected and then the cell-homing and differentiation were investigated. The hGFP-transfected hMSCs were comparatively tested from 1 to 4 passages in vitro. The efficiency of the cell-transfection was followed and 3-hour and 6-hour transfections in the 1 passage were similar to non-transfected cells for the first 3 days. These cells were used for post-irradiated, craniofacial-, and head and neck-related tissue regeneration. And the keloid clinical series were also tested in terms of human mesenchymal stem cells. In the post-irradiation in vivo, there was remarkable efficiency of mesenchymal stem cells. Also, hMSCs demonstrated to the relationship to the fibroproliferative disease, FPD. These cells may be used for emergency radiation diseases as stockpiles since these cells are frozen and hGFP-labeling are consistent after thawing. The human mesenchymal stem cells are now used for cell-bank cells in the Western countries. Thus, the extensions of the cells in this direction should be considered in Japan as well. hGFP-labeled hMSCs are in vivo tested in the decreased blood flows and impaired nerves demonstrated the significant recovery as well as the cranial bone-defect models temporally and spaciously. Therefore, further preclinical and clinical tests should be planned with using autologous tissue-derived stem cells.
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[Journal Article] PAX9 and TGFB3 are linked to susceptiblility to nonsyndromic cleft lip with or without cleft palate in the Japanese : Population-based and family-based candidate gene analyses2006
Author(s)
Ichikawa, E., Watanabe, A., Nakano, Y., Akita, S., Hirano, A., Kinoshita, A., Kondo, S., Kishino, T., Uchiyama, T., Niikawa, N., Yoshiura, KI
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Journal Title
J Hum Genet 51
Pages: 38-46
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Elevated circulating leukemia inhibitory factor levels in burn patients : marker of mortality2006
Author(s)
Akita, S., Akino, K., Ren, SG., Melmed, S., Imaizumi, T., Hirano, A
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Journal Title
J Burn Care Res(formerly J Burn Care Rehabil) 27
Pages: 221-225
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Attenuation of cysteinyl leukotrienes induces human mesenchymal stem cells differentiation2006
Author(s)
Akino, K., Mineda, T., Mori, N., Hirano, A., Imaizumi, T., Akita, S
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Journal Title
Wound Repair Regen 14
Pages: 343-349
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Human mesenchymal stem cells may involve in keloid pathogenesis
Author(s)
Akino, K., Akita, S., Yakabe, A., Mineda, T., Hayashi, T., Hirano, A
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Journal Title
Int J Dermatol. (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Emergency reconstructive and regenerative plastic surgery for systemic and local radiation injury2006
Author(s)
Akita, S., Akino, K., Ohtsuru, A., Hirano, A., Yamashita, S
Organizer
11th Coordination and planning meeting of the WHO REMPAN collaborating centers and liazon institutions
Place of Presentation
Special Lecture, Kiev, Ukraine
Year and Date
20060400
Description
「研究成果報告書概要(欧文)」より
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