• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

Craniofecial bone reganeration by mesenchymal stem cells of the craniofacial origin

Research Project

Project/Area Number 18390478
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionNagasaki University

Principal Investigator

HIRANO Akiyoshi  Nagasaki University, Graduate School of Biomedical Sciences, Professor (90208835)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Mutsuhisa  Nagasaki University, Graduate School of Engineering, Professor (20039689)
AKITA Sadanori  Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (90315250)
NAGAYAMA Yuji  Nagasaki University, Graduate School of Biomedical Sciences, Professor (30274632)
Project Period (FY) 2006 – 2007
Keywordstransfection / human bone marrow-derived mesenchvmal stem cells / tissue regeneration and healing / congenital anomaly / life-style oriented diseases / bone maturation marker / vessel and nerve regeneration / facial bone growth
Research Abstract

Human bone marrow derived mesenchymal stem cell, hMSCs were tested for post-grafting kinetics in the craniofacial regions, human Green Fluorescent Protein (hGFP)CDNA was trasfected and then the cell-homing and differentiation were investigated. The hGFP-transfected hMSCs were comparatively tested from 1 to 4 passages in vitro. The efficiency of the cell-transfection was followed and 3-hour and 6-hour transfections in the 1 passage were similar to non-transfected cells for the first 3 days. These cells were used for post-irradiated, craniofacial-, and head and neck-related tissue regeneration. And the keloid clinical series were also tested in terms of human mesenchymal stem cells. In the post-irradiation in vivo, there was remarkable efficiency of mesenchymal stem cells. Also, hMSCs demonstrated to the relationship to the fibroproliferative disease, FPD. These cells may be used for emergency radiation diseases as stockpiles since these cells are frozen and hGFP-labeling are consistent after thawing. The human mesenchymal stem cells are now used for cell-bank cells in the Western countries. Thus, the extensions of the cells in this direction should be considered in Japan as well. hGFP-labeled hMSCs are in vivo tested in the decreased blood flows and impaired nerves demonstrated the significant recovery as well as the cranial bone-defect models temporally and spaciously. Therefore, further preclinical and clinical tests should be planned with using autologous tissue-derived stem cells.

  • Research Products

    (19 results)

All 2007 2006 Other

All Journal Article (11 results) (of which Peer Reviewed: 3 results) Presentation (6 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Acceleration of sensory neural regeneration and would healing with human mesenchymal stem cells in immunodeficient rats2007

    • Author(s)
      Imaizumi T, Akita S, Akino K, Hirano A
    • Journal Title

      Stem Cells 25

      Pages: 2956-2963

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Acceleration of sensory neural regeneration and would healing with human mesenchyraal stem cells in immunodeficient rats2007

    • Author(s)
      Iraaizumi, T., Akita, S., Akino, K., Hirano, A
    • Journal Title

      Stem Cells 25

      Pages: 2956-2963

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Usefulness of simultaneous premaxillary osteotomy and bone grafting in the bilateral clefts2006

    • Author(s)
      Akita S, Hirano A.
    • Journal Title

      J Craniofac Surg 17

      Pages: 291-296

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Sleep disturbances detected by a sleep apnea monitor in craniofacial surgical patients2006

    • Author(s)
      Akita S, Anraku K, Tanaka K, Yano H, Hirano A
    • Journal Title

      J Craniofac Surg 17

      Pages: 44-49

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Sleep disturbances detected by a sleep apnea monitor in craniofacial surgical patients2006

    • Author(s)
      Akita, S., Anraku, K., Tanaka, K., Yano, H., Hirano, A
    • Journal Title

      J Craniofac Surg 17

      Pages: 44-49

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Usefulness of simultaneous premaxillary osteotomy and bone grafting in the bilateral clefts2006

    • Author(s)
      Akita, S., Hirano, A
    • Journal Title

      J Craniofac Surg 17

      Pages: 29-296

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] PAX9 and TGFB3 are linked to susceptiblility to nonsyndromic cleft lip with or without cleft palate in the Japanese : Population-based and family-based candidate gene analyses2006

    • Author(s)
      Ichikawa, E., Watanabe, A., Nakano, Y., Akita, S., Hirano, A., Kinoshita, A., Kondo, S., Kishino, T., Uchiyama, T., Niikawa, N., Yoshiura, KI
    • Journal Title

      J Hum Genet 51

      Pages: 38-46

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Elevated circulating leukemia inhibitory factor levels in burn patients : marker of mortality2006

    • Author(s)
      Akita, S., Akino, K., Ren, SG., Melmed, S., Imaizumi, T., Hirano, A
    • Journal Title

      J Burn Care Res(formerly J Burn Care Rehabil) 27

      Pages: 221-225

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Lower extremity reconstruction after necrotizing fasciitis and necrotic skin lesions using a porcine-derived skin substitute2006

    • Author(s)
      Akita, S., Tanaka, K., Hirano, A
    • Journal Title

      J Plast Reconstr Aesthet Surg(formerly Br J Plast Surg) 59

      Pages: 759-763

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Attenuation of cysteinyl leukotrienes induces human mesenchymal stem cells differentiation2006

    • Author(s)
      Akino, K., Mineda, T., Mori, N., Hirano, A., Imaizumi, T., Akita, S
    • Journal Title

      Wound Repair Regen 14

      Pages: 343-349

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human mesenchymal stem cells may involve in keloid pathogenesis

    • Author(s)
      Akino, K., Akita, S., Yakabe, A., Mineda, T., Hayashi, T., Hirano, A
    • Journal Title

      Int J Dermatol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Stem cell therapy and Plastic surgical management for local radiation2007

    • Author(s)
      Akita, S
    • Organizer
      2nd International Seminar-"Radiation Medicine in Research and Practive" Wuerzburg
    • Place of Presentation
      Germany, Invited Symposium
    • Year and Date
      20071004-05
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 当科における片側唇・顎・口蓋裂の治療-今日の治療法に至った変遷と長期成績2007

    • Author(s)
      平野 明喜
    • Organizer
      第50回日本形成外科学会総会・学術集会(シンポジウム)
    • Place of Presentation
      東京
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Emergency reconstructive and regenerative plastic surgery for systemic and local radiation injury2006

    • Author(s)
      Akita, S., Akino, K., Ohtsuru, A., Hirano, A., Yamashita, S
    • Organizer
      11th Coordination and planning meeting of the WHO REMPAN collaborating centers and liazon institutions
    • Place of Presentation
      Special Lecture, Kiev, Ukraine
    • Year and Date
      20060400
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] basic fibroblast growth factor in scar management2006

    • Author(s)
      Akita, S
    • Organizer
      1st Scar Meeting, Montpellier, France, Guest speaker
    • Year and Date
      20060300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Indication of maxillo-mandibular osteotomies for Class III Malocclusion2006

    • Author(s)
      Akiyoshi, Hirano
    • Organizer
      The 1st joint meeting of the Japanese orthodontic society and the Korean Association of orthodontics
    • Place of Presentation
      Sapporo Japan
    • Year and Date
      2006-09-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Trans-facial approach to skull base tumors - The role of plastic surgeons2006

    • Author(s)
      Akiyoshi, Hirano
    • Organizer
      The 16th China-Japan Joint Meeting on Plastic Surgery
    • Place of Presentation
      Harbin, China
    • Year and Date
      2006-08-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Radiation Risk and Perspectives2007

    • Author(s)
      Akita S, Akino K, Hirano A, Ohtsuru A, Yamashita S
    • Total Pages
      298
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.med.nagasaki-u.ac.jp/plastics/

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi