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2008 Fiscal Year Final Research Report

Establishment of organ culture system for tooth regeneration using pharyngeal teeth of medaka and its use for the exploration of dental stem cells

Research Project

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Project/Area Number 18390485
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TAKANO Yoshiro  Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (90126425)

Co-Investigator(Kenkyū-buntansha) TABATA Makoto  東京医科歯科大学, 大学院・医歯学総合研究学科, 准教授 (20243248)
BABA Otto  東京医科歯科大学, 大学院・医歯総合研究学科, 助教 (90251545)
ISEKI Hachiro  東京医科歯科大学, 技術専門, 職員 (00401373)
Project Period (FY) 2006 – 2008
Keywordsメダカ / 咽頭歯 / 器官培養 / 歯の再生 / 実験モデル
Research Abstract

本研究は常時脱落と交換を繰り返すメダカ咽頭歯を用いた歯の再生実験モデルの確立を目的に計画されたものであり、咽頭歯骨部の構造基盤と歯の発生・発達・交換の全容を初めて光顕、電顕レベルで明らかにした。更に同部の器官培養系を立ち上げて歯胚とその関連細胞の分化・機能維持が可能な培養条件を決定した。本メダカ咽頭歯骨部器官培養系は歯の発生・発達に伴う機能分子の消長とvivoでの確認が困難な咽頭歯脱落・交換のリアルタイム観察が可能であり、歯の再生研究への活用が期待される。

  • Research Products

    (38 results)

All 2009 2008 2007 2006 Other

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (14 results) Book (4 results)

  • [Journal Article] Phosphatase actions at the site of appositional mineralization in bisphosphonate-affected bones of the rat2008

    • Author(s)
      Yi Li, Hiroto Nakayama, Takuya Notani, Masud Ahmad, Makoto J. Tabata and Yoshiro Takano
    • Journal Title

      J Medical and Dental Sciences 55(3,4)

      Pages: 255-265

    • Peer Reviewed
  • [Journal Article] An immunohistochemical and ultrastructural study of the pericellular matrix ofuneroded hypertrophic chondrocytes in the mandibular condyle of aged c-src-deficient mice2008

    • Author(s)
      Shunichi Shibata, Otto Baba, Tsuyoshi Oda, Tamaki Yokohama-Tamaki, Chunlin Qin, William T. Butler, Yasunori Sakakura and Yoshiro Takano
    • Journal Title

      Arch Oral Biol 53(3)

      Pages: 220-230

    • Peer Reviewed
  • [Journal Article] E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro2008

    • Author(s)
      Ken-ichi Katsube, Naruhito Matsuda, Kazuo Kishi, Yoshiro Takano, Hachiro Iseki, Yuko Katsuki, Akira Yamaguchi, Hideo Nakajima, Shuji Mikami, Makio Mukai, Tatsuo Nakajima
    • Journal Title

      J Dermatol Sci

    • Peer Reviewed
  • [Journal Article] Multinucleated osteoclasts in medaka as evidence of active bone remodeling2007

    • Author(s)
      Nemoto Y, Higuchi K, Baba O, Kudo A, Takano Y
    • Journal Title

      Bone 40

      Pages: 399-408

    • Peer Reviewed
  • [Journal Article] Diverse effects of C-src deficiency on molar tooth development and eruption in mice2007

    • Author(s)
      Miyata A, Baba O, Oda T, Ishikawa I, Takano Y
    • Journal Title

      Arch Histol Cytol 70

      Pages: 63-78

    • Peer Reviewed
  • [Journal Article] Glycogen Phosphorylase in Glycogen-Rich Cells Is Involved in Energy Supply for Ion Regulation in Fish Gill Epithelia2007

    • Author(s)
      Tseng Y-C, Huang C-J, Chang J, Teng W-Y, Baba O, Fann M-J, Hwang P-P
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol 293

      Pages: 482-491

    • Peer Reviewed
  • [Journal Article] Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia(Oreochromis mossambicus)during acclimation to seawater2007

    • Author(s)
      Chang J C-H, Wu S-M, Tseng Y-C, Lee Y-C, Baba O, Hwang P-P
    • Journal Title

      J Exp Biol 210

      Pages: 3494-3504

    • Peer Reviewed
  • [Journal Article] The teleost intervertebral region acts asa growth center of the centrum : in vivo visualization of osteoblasts and their progenitors in transgenic fish2007

    • Author(s)
      Inohaya K, Takano Y, Kudo A
    • Journal Title

      Dev Dyn 236

      Pages: 3031-3046

    • Peer Reviewed
  • [Journal Article] Effect of vibration on osteoblastic and osteoclastic activities : Analysis of bone metabolism using goldfish scale as a model for bone2007

    • Author(s)
      Suzuki N, Kitamura, K, Nemoto T Shimizu N, Wada S, Kondo T, Tabata MJ, Sodeyama F, Ijiri K, Hattori A
    • Journal Title

      Adv Space Res 40

      Pages: 1711-1721

    • Peer Reviewed
  • [Journal Article] 魚鱗2007

    • Author(s)
      田畑 純, 鈴木信雄, 服部淳彦
    • Journal Title

      硬組織研究と再生研究のフロンティア細胞 39(2)

      Pages: 55-57

  • [Journal Article] Remodeling of Acellular Bone in Japanese Medaka(Oryzias latipes)and its Possible Contribution to Mineral Regulation of the Body2006

    • Author(s)
      Takano Y, Nakano Y, Katogi R, Nemoto Y, Kudo A
    • Journal Title

      Proceedings of 8th International Conference of the Chemistry and Biology of Mineralized Tissues

      Pages: 13-15

    • Peer Reviewed
  • [Journal Article] Immunoloclization of dentprotein 1(DMP1)fragments in rat molar2006

    • Author(s)
      Baba 0, Qin C, Brunn JC, Terashima T, Bonewald L, Butler WT
    • Journal Title

      Proceedings of 8th International Conference ofChemistry and Biology of MineralizTissues

      Pages: 143-146

    • Peer Reviewed
  • [Journal Article] Unique Similarities between Dentin Matrix Protein 1 and Dentin Sialophosphoprotein2006

    • Author(s)
      Qin C, Baba O, Brunn JC, McKee MD, Bonewald L, Butler WT
    • Journal Title

      Proceedings of 8th International Conference of the Chemistry and Biology of Mineralized Tissues

      Pages: 174-177

    • Peer Reviewed
  • [Journal Article] Proteolysis on maturing enamel surface as shown by gel coating methods2006

    • Author(s)
      Takano Y, Nakano Y, Yamamoto-Shuda Y, Baba O, Terashima T
    • Journal Title

      Eur J Oral Sci 114

      Pages: 52-58

    • Peer Reviewed
  • [Journal Article] Formation of acellular cementum-like layers with and without extrinsic fiber insertion along inert bone surfaces of aging c-Src gene knockout mice2006

    • Author(s)
      Baba O, Miyata A, Abe T, Shibata S, Nakano Y, Terashima T, Oda T, Kudo A, Takano Y
    • Journal Title

      Eur J Oral Sci 114

      Pages: 524-534

    • Peer Reviewed
  • [Journal Article] 宇宙におけるメダカ骨代謝2006

    • Author(s)
      工藤明, 石岡憲昭, 内田智子, 大森克徳, 河野靖, 高野吉郎, 永松愛子, 夏井坂誠, 西川和香, 藤本信義, 益川充代, 村上敬司
    • Journal Title

      Space Utiliz Res 22

      Pages: 241-243

    • Peer Reviewed
  • [Journal Article] A Nerve-epithelium Interrelation in the Periodontal Ligament of Guinea pig Teeth2006

    • Author(s)
      Jayawardena CK, Takano Y
    • Journal Title

      Arch Oral Biol 51

      Pages: 587-595

    • Peer Reviewed
  • [Journal Article] Structural features of incremental line-oike striations in mandibular condylar cartilage of c-src-deficient mice2006

    • Author(s)
      Shibata S, Oda T, Abe T, Yamashita Y, Takano Y
    • Journal Title

      Arch Oral Biol 51

      Pages: 951-959

    • Peer Reviewed
  • [Journal Article] The existence of CD11c' sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties2006

    • Author(s)
      Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M
    • Journal Title

      International Immunol 18

      Pages: 1375-1384

    • Peer Reviewed
  • [Journal Article] Development and Innervation of Subcutaneously Transplanted Molar Tooth Germs in the Rat

    • Author(s)
      Higuchi K, Peerapong S, Terashima T, Nakayama H, Notani T, Iseki H, Baba O and Takano Y
    • Journal Title

      Eur J Oral Sci 116

      Pages: 324-333

    • Peer Reviewed
  • [Presentation] Histological evaluation of osteoconductivity and fate of anorganic bovine bone fragments(Bio-Oss[○!R]) applied for sinus augmentation preceding implant2009

    • Author(s)
      Tamaki Hitoshi, Nakayama Hiroto, Takano Yoshiro
    • Organizer
      Academy of Osseointegration Annual Meeting
    • Place of Presentation
      San Diego, CA
    • Year and Date
      20090226-28
  • [Presentation] Teeth in Edar deficient medaka fish : an animal model of anhidrotic ectodermal dysplasia2009

    • Author(s)
      A.D.S. ATUKORALA, Kazunori HIGUCHI, Masayuki YOSHIMI, Makoto J TABATA, Otto BABA, Yoshiro TAKANO
    • Organizer
      114^<th> Japanese Association of Anatomists
    • Place of Presentation
      Okayama
    • Year and Date
      2009-05-28
  • [Presentation] Cement lines and bone quality-A lesson from studies of long-lasting cement lines in osteopetrotic bones of aging c-src deficient mice2009

    • Author(s)
      Yoshiro Takano
    • Organizer
      Invited lecture, Material Research Society Spring Meeting 2009
    • Place of Presentation
      San Francisco
    • Year and Date
      2009-04-14
  • [Presentation] エナメル上皮細胞と歯髄細胞を用いた三次元・重層培養にける組織再構築と細胞相互誘導2009

    • Author(s)
      田畑純、野谷拓也、井関八郎、中山博登、馬場麻人、高野吉郎
    • Organizer
      第114回日本解剖学会シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-30
  • [Presentation] Cell Dynamics in Calcium Regulating Epithelium of the Enamel Organ during Tooth Enamel Formation2008

    • Author(s)
      Takano Y
    • Organizer
      The 7th Federation Meeting of Korean Basic Dental Scientists
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      20081202-03
  • [Presentation] Impaired Tooth Development In Reduced Scale Medaka Mutant2008

    • Author(s)
      Atukorala ADS, Higuchi K, Tabata MJ, Baba O, Mitani H , Takano Y
    • Organizer
      56th General Session of the JADR
    • Place of Presentation
      Nagoya,Japan
    • Year and Date
      20081129-30
  • [Presentation] Three-dimensional & layered culture method for tooth induction and development2008

    • Author(s)
      Notani T, Tabata MI, Baba O, Takano Y
    • Organizer
      56th General Session of the JADR
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20081129-30
  • [Presentation] Provisional mineralization layer in the predentin of murine teeth2008

    • Author(s)
      Ahmad M, Iseki H, Baba O, Tabata MJ, Takano Y
    • Organizer
      56th General Session of the JADR
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20081129-30
  • [Presentation] ラット歯髄におけるFGF18の発現 : 加読召RT-PCRによる検出2008

    • Author(s)
      馬場麻人、太田正人、寺島達夫、田畑純、高野吉郎
    • Organizer
      第50回歯科基礎医学会
    • Place of Presentation
      東京
    • Year and Date
      20080923-25
  • [Presentation] Enamel matrix derivative(EMD)の歯根吸収抑制活性はアメロゲニンが担う2008

    • Author(s)
      八木優子、須田直人、森山啓司、山越康雄、馬場麻人
    • Organizer
      第67回日本矯正歯科学会大会
    • Place of Presentation
      千葉
    • Year and Date
      20080916-18
  • [Presentation] 脊椎骨融合を示すダルマメダカ変異体の原因遺伝子のAT FA Analysis of the Dharma- medaka mutant, which shows a defect in formation of vertebral column2008

    • Author(s)
      猪早敬二、高野吉郎、工藤明
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学
    • Year and Date
      20080905-07
  • [Presentation] Analysis of the Dharma-medaka mutant, which shows a defect in formation and/or maintenance of intervertebral region2008

    • Author(s)
      Inohaya K, Takano Y, Kudo A
    • Organizer
      第41回日本発生生物学会
    • Place of Presentation
      徳島
    • Year and Date
      20080528-30
  • [Presentation] 石灰化機構から象牙芽細胞と骨芽細胞の異同を探る、日本解剖学会シンポジウム象牙芽細胞と骨芽細胞の違いを考える2008

    • Author(s)
      高野吉郎
    • Organizer
      第113回日本解剖学会学術大会
    • Place of Presentation
      大分大学
    • Year and Date
      20080327-29
  • [Presentation] 東京医科歯科大学大学院硬組織構造生物学分野 : 小型硬骨魚メダカ無細胞骨格系における骨形成と骨吸収2006

    • Author(s)
      高野吉郎
    • Organizer
      第114回日本解剖学会シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2006-03-28
  • [Book] 新・う蝕の科学(浜田茂幸, 大島隆)2006

    • Author(s)
      高野吉郎
    • Publisher
      医歯薬出版
  • [Book] 口腔組織・発生学2006

    • Author(s)
      高野吉郎
    • Publisher
      医歯薬出版
  • [Book] 染色・バイオイメージングの実験ハンドブック2006

    • Author(s)
      高野吉郎
    • Publisher
      羊土社
  • [Book] リープコット歯科学のための解剖学2006

    • Author(s)
      高野吉郎
    • Publisher
      西村書店

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Published: 2010-06-10   Modified: 2016-04-21  

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