2007 Fiscal Year Final Research Report Summary
Development of gene delivery system using nanocoloid and its application for gene targeting therapy
| Project/Area Number |
18390531
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHINDOH Masanobu Hokkaido University, Graaduate School of Dental Medicine, Professor (20162802)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masanobu Hokkaido University, Institute for Genetic Medicine, Associate Professeor (80241321)
FUGETSU Bunshi Hokkaido University, Graduate School of Environmental Science & Faculty of Environmetal Earth Science, Professor (40281844)
HIGASHINO Fumihiro Hokkaido University, Graduate School of Dental Medicine, Associate Professor (50301891)
HIDA Kyoko Hokkaido University, Graduate School of Dental Medicine, Assistant Professor (40399952)
KITAMURA Tetsuya Hokkaido University, Graduate School of Dental Medicine, Research Associate (00451451)
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| Project Period (FY) |
2006 – 2007
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| Keywords | nanocoloid / cell-specific gene transfer / adrenomedullin |
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| Research Abstract |
Trials of gene therapies have been carried out for malignant tumors and congenital gene disorders,; however, there are problems such as the low efficiency and risk for applying human being, and ideal methods of gene therapy are not yet developed.. We designed nanocoloid that enclosed expression plasmid inside and, ligand peptide outside of small particle for development of a specific gene delivery system. When We searched expression of EGFR in oral squamous cell carcinoma cell lines (9.22, HSC2, HSC3, HSC4, Ca SAS) by Western blot, and all cell lines expressed EGFR We examined the binding affinity of EGFR and synthesized EGF peptide by BireCore X. As a result, it became clear that synthesized peptide bound to EGFR. Next, we examined the induction effect of nanocoloid to oral carcinoma cell lines. The efficiency of transfection was approximately 30%, and we aimed to improve the efficiency. We next examined the effects of adrenomedullin antagonist that has the ability to inhibit angiogenesis. The vector that has the sequence for inhibitory effects on aderenomedullin reduced the Growth of tumor xenografts in nude mice.
These results imply that specific gene delivery for tumor environment could be the therapeutic agents for solid cancers.
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[Journal Article] Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonist.2007
Author(s)
Miseki T, Kawakami H, Natsuizaka M, Demanin S, Cui H Y, Chen J, Fu Q, Okada F, Shindoh M, Higashino F, Asaka M, Hamuro J, Kobayashi M
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Journal Title
Cancer Gene Ther 14
Pages: 39-44
Description
「研究成果報告書概要(欧文)」より
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