Mechanism of gene expression of bone sialoprotein and transcription factors which control periodontal regeneration

2008 Fiscal Year Final Research Report

• Project/Area Number: 18390563
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Nihon University
• Principal Investigator: OGATA Yorimasa Nihon University, 松戸歯学部, 教授 (90204065)
• Co-Investigator(Kenkyū-buntansha): NAKAO Sumi 日本大学, 松戸歯学部, 助手 (20102577) ; MASUNAGA Hiroshi 日本大学, 松戸歯学部, 講師 (40229381)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Yohei 日本大学, 松戸歯学部, 講師 (30434088) ; TAKAI Hideki 日本大学, 松戸歯学部, 助教 (30453898)
• Project Period (FY): 2006 – 2008
• Keywords: 遺伝子プロモーター / 転写調節 / 石灰化 / 骨芽細胞 / 遺伝子発現 / 転写因子 / 成長因子 / ホルモン

Research Abstract

骨シアロタンパク質(BSP)は、アパタイト結晶形成能を有することから、歯周組織再生に重要な役割を果たすと考えられる。骨芽細胞様細胞を副甲状腺ホルモン(PTH; 10nM)で刺激すると、3時間後にBSPmRNA, タンパク質量が増加した。ヒトBSP遺伝子プロモーターの転写活性はPTH刺激(10 nM、3~6時間)で上昇し、プロモーター中の2つのCRE配列への核内タンパク質の結合は、PTH(10 nM)刺激3時間後に増加した。以上の結果は、PTHがBSPの遺伝子発現を増加させ、歯周組織再生を増強する可能性を示唆すると思われる。

Research Products

• [Journal Article] Regulation of human bone sialoprotein gene transcription by platelet-derived growth factor-BB (2009)
  • Author(s): M. Mezawa, S. Araki, H. Takai, Y. Sasaki, S. Wang, X. Li, D. Kim, Y. Nakayama, Y. Ogata
  • Journal Title: Gene 435 Pages: 80-87
  • Peer Reviewed
• [Journal Article] Parathyroid hormone regulation of the human bone sialoprotein gene transcription is mediated through two cAMP response elements (2009)
  • Author(s): S. Araki, M. Mezawa, Y. Sasaki, L. Yang, Z. Li, H. Takai, Y. Nakayama, Y. Ogata
  • Journal Title: J. Cell. Biochem 106 Pages: 618-625
  • Peer Reviewed
• [Journal Article] 骨シアロタンパク質(BSP)の転写調節に対するCO2レーザーの効果 (2008)
  • Author(s): 佐々木庸子, 荒木正大, 目澤優, ZhitaoWang, 金子博寿, 小方頼昌
  • Journal Title: 日本歯周誌 50 Pages: 176-184
  • Peer Reviewed
• [Journal Article] AP1 Binding Site is Another Target of FGF2 Regulation of Bone Sialoprotein Gene Transcription (2008)
  • Author(s): H. Takai, S. Araki, M. Mezawa, D. Kim, X. Li, L. Yang, Z. Li, Z. Wang, Y. Nakayama and Y. Ogata
  • Journal Title: Gene 410 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Bone sialoprotein and its transcriptional regulatory mechanism (2008)
  • Author(s): Y. Ogata
  • Journal Title: J Perio Res 43 Pages: 127-135
  • Peer Reviewed
• [Journal Article] Androgen Receptor stimulates Bone Sialoprotein (BSP) Gene Transcription via cAMP response element and activator protein 1/glucocorticoid response elements (2007)
  • Author(s): H. Takai, Y. Nakayama, D. Kim, M. Arai, S. Araki, M. Mezawa, Y. Nakajima, N. Kato, H. Masunaga and Y. Ogata
  • Journal Title: Journal of Cellular Biochemistry 102 Pages: 240-251
  • Peer Reviewed
• [Journal Article] Effects of Quercetin and Quercetin 3-Glucuronide on the Expression of Bone Sialoprotein Gene (2007)
  • Author(s): D. Kim, H. Takai, M. Arai, S. Araki, M. Mezawa, Y. Kawai, K. Murota, J. Terao and Y. Ogata
  • Journal Title: Journal of Cellular Biochemistry 101 Pages: 790-800
  • Peer Reviewed
• [Journal Article] 骨シアロタンパク質(BSP)遺伝子の転写に対するアンドドゲン受容体の効果 (2007)
  • Author(s): 高井英樹, 小方頼昌
  • Journal Title: 日本歯周誌 49 Pages: 27-36
  • Peer Reviewed
• [Journal Article] ブレストロンを用いた口臭と臨床パラメーターの関係について (2007)
  • Author(s): 大橋顕二郎, 鈴木桃子, 今荘雅秀, 小山朱美, 齋藤綾一朗, 若林健史, 増永浩, 小方頼昌
  • Journal Title: 日本歯周誌 49 Pages: 191-197
  • Peer Reviewed
• [Journal Article] Effects of Reactive Oxygen Species (ROS) on Antioxidant System and Osteoblastic Differentiation in MC3T3-E1 cells (2007)
  • Author(s): M. Arai, Y. Shibata, K. Pugdee, Y. Abiko and Y. Ogata
  • Journal Title: IUBMB Life 59 Pages: 27-33
  • Peer Reviewed
• [Presentation] Androgen Receptor Regulates Bone Sialoprotein Gene Expression (2008)
  • Author(s): H, Takai, Y, Nakayama, D, Kim, S, Araki, M, Mezawa, N, Kato, Y, Ogata
  • Organizer: IADR General Session
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080702-05
• [Presentation] cAMP Regulates BSP Expression in Human Breast Cancer Cells (2008)
  • Author(s): D. Kim, H. Takai, N. Kato, Y. Sasaki, S. Wang, Y. Nakayama, Y. Ogata
  • Organizer: IADR General Session
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080702-05
• [Presentation] Insulin-like growth Factor-I regulates bone sialoprotein gene expression (2007)
  • Author(s): Y. Nakayama, N. Kato, H. Takai, D. Kim, M. Arai, S. Araki, M.Mezawa, Y. Ogata
  • Organizer: 9th International Conference of Chemistry and Biology of Mineralized Tissues
  • Place of Presentation: Austin USA
  • Year and Date: 20071104-08
• [Presentation] Regulation of human bone sialoprotein gene transcription by platelet-derived growth factor (2007)
  • Author(s): M. Mezawa, S. Araki, D. Kim, H. Takai, Z. Wang, X. Li, N. Kato, Y.Nakayama, Y. Ogata
  • Organizer: 9th International Conference of Chemistry and Biology of Mineralized Tissues
  • Place of Presentation: Austin USA
  • Year and Date: 20071104-08
• [Presentation] Regulation of BSP Gene Transcription by Amelogenins and Sheath proteins (2007)
  • Author(s): Y. Ogata, Y. Nakajima, L. Yang, Z. Li, Z. Wang, X. Li, M. Mezawa, S. Araki, Y. Nakayama, S. Oida, M. Fukae
  • Organizer: 9th International Conference of Chemistry and Biology of Mineralized Tissues
  • Place of Presentation: Austin USA
  • Year and Date: 20071104-08