2007 Fiscal Year Final Research Report Summary
Functional and morphological analysis of a novel peptide relaxin 3 in the central nervous system
| Project/Area Number |
18500268
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TANAKA Masaki Kyoto Prefectural University of Medicine, Dep. Cell Biol, Instit for Neurological Diseases and Geriatrics, Associate Professor (80264753)
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| Co-Investigator(Kenkyū-buntansha) |
IIJIMA Norio Kyoto Prefectural Univ, Med, Dep. Anatomy, Research Associate (00285248)
WATANABE Yoshihisa Kyoto Prefectural Univ, Med, Dep. Cell Biol, Instit for Neurological Diseases and Geriatrics, Associate (50363990)
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| Project Period (FY) |
2006 – 2007
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| Keywords | relaxin3 / INSL7 / development / serotonin / gene expression / cAMP / neuro2a / Corticotropin releasing factor |
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| Research Abstract |
We have been investigating physiological function of a newly identified brain peptide at 2002 called, relaxin3 or insulin like peptide 7 (INSL7) which belongs to the insulin super family. Already we reported that relaxin 3 is dominantly expressed in neurons of the brainstem called nucleus incertus (NI) in the median dorsal pons (Tanaka M, et. al. Eur J Neurosci. 2005).
1. First year, we studied the developmental and aged expression of relaxin 3 in the NI. Relaxin 3 mRNA was detected from embryonic day 18 by RT-PCR and in situ hybridization histochemistry. It was found at peptide level from birth. In aging, the number of relaxin 3 expressing neurons and level of expression at 18 month were decreased compared with those at 2 month. We also investigated the influence of serotonin (5-HT) because NI was innervated by 5-HT fibers and dorsal raphe nucleus, which contains abundant 5-HT neurons, is located just dorsal to the NI. Relaxin 3 neurons in the NI coexpressed 5-HT1A receptor and the tre
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atment of 5-HT depletor PCPA increased the relaxin 3 mRNA level. These results suggest 5-HT negatively regulate the relaxin 3 expression in the NI. The outcome of this study was published in the journal (Miyamoto et al, Regul Pept, 2008).
2. Second year, as relaxin 3 mRNA was increased in various conditions such as stress and PCPA treatment, we started to study the mechanism of relaxin 3 transcription. We found that relaxin 3 was expressed in a mouse neuroblastoma cell line, Neuro2a, and investigated the intracellular signaling that activated relaxin 3 gene transcription in vitro. Using a clone stably transfected with a relaxin 3 promoter-EGFP gene, we observed that dibutyryl cyclic AMP and forskolin increased the relaxin 3 promoter activity. These increases were inhibited by pretreatment with a specific PKA inhibitor, H89. Moreover, the promoter activity was enhanced by CRF treatment after expression of CRF-Rl receptor on the cells. Taken together, these results indicate that relaxin 3 transcription is activated via the cAMP-PKA pathway in the downstream of CRF-Rl. The above results are now submitted to J Neurosci Res. Less
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Research Products
(31 results)
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[Journal Article] Cleavage of normal and pathological forms of alpha-synuclein by neurosin in vitro2008
Author(s)
Kasai, T., Tokuda, T., Yamaguchi, N., Watanabe, Y., Kametani, F., Nakagawa, M., Mizuno, T
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Journal Title
Neurosci Lett 436
Pages: 52-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induction of CB1 cannabinoid receptor by inflammation in primary afferent neurons facilitates antihyperalgesic effect of peripheral CB1 agonist2006
Author(s)
Amaya. F. Shimosato, G., Kawasaki, Y., Hashimoto, S., Tanaka, Y., Ji, R., Tanaka, M
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin2006
Author(s)
Mitsui, S., Nakamura, T., Okui, A., Kominami, K., Uemura, H., Yamaguchi, N
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Journal Title
FEBS J 273
Pages: 3678-3686
Description
「研究成果報告書概要(欧文)」より
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