2007 Fiscal Year Final Research Report Summary
Analysis of living synaptic proteins in the plastic modification of synapse.
| Project/Area Number |
18500272
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
EBIHARA Tatsuhiko National Institute of Advanced Industrial Science and Technology, Neuroscience Research Institute, Researcher (00344119)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Neural regeneration / Neural plasticity / Post synaptic density (PSD) |
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| Research Abstract |
Before this research term, I made some transgenic mice that visualize some synaptic proteins. In hippocampal pyramidal cells. From the analysis of these mice, I need some transgenic mice that express synaptic marker proteins in the restricted portion of pyramidal cells for the purpose to analyze a living hippocampal circuit or a brain.
I made some transgene DNAs that have promoter-loxP sequence-marker protein, and made some Tg mice. These Tg mice can express restrictively when some portion of pyramidal cells accept Cre recombinase expression and are recombined with loxP sequence. In the "promoter-loxP-FGFP transgeinc mice, I saw restricted expression by Adeno wirus Cre recombinase infection. It visualized GA1-CA3 connection. Next, I made loxP-PSD95-EGFP, loxP-PSD95-Venus Tg mice.
For expression of living mice, adeno virus needs P2A grade mice institute, I tuned in utero electro poration method(P1A) for expressing cre recombinase in restricted pyramidal cells. In this research term, I saw restricted expression using this IUEP-loxP Tg system.
I also tuned analyzing system around 2 photon microscope.
Next step, I want to analyze synaptic dynamism by using these system and methods that fixed by this research grant.
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Research Products
(2 results)
