2007 Fiscal Year Final Research Report Summary
The functional analysis of Sox-6 in neural stem cells for potential application in regenerational medicine
| Project/Area Number |
18500299
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Keio University |
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Principal Investigator |
OHTA Shigeki Keio University, School of Medicine, Instructor (20365406)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Msahiro Keio University, School of Medicine, Assistant Professor (20217508)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Sox6 / Neural Stem Cell / Regenerational medicine |
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| Research Abstract |
The detailed function of Sox6 in the CNS including neural stem/progenitor cells (NSPCs) has been unclear. In this study, we analyzed the expression of Sox6 in the mouse developing brain and the function in NSPCs. By immunohistochemical analysis, we found that Sox6 was expressed in the ventricular zone of mouse telencephalon at 14.5 dpc, co-localizing with NSPCs's markers, nestin and Sox2. The expression of Sox6 in undifferentiated neurospheres also showed the co-localization with nestin, suggesting that Sox6 may play some roles in NSPCs. To evaluate the function of Sox6 in NSPCs, we performed a neurosphere forming assay in vitro. Over-expression of Sox6 using retrovirus vector in neurospheres caused an increase in the number of secondary neurospheres without changing the proliferation ability as determined by BrdU incorporation assay. Retrovirally over-expressed Sox6 also increased the expression of Hes-1, Bcl-2 and phosphorylated-Akt in the neurospheres. These results suggest that Sox6 is expressed in NSPCs in the developing CNS and involved in the maintenance and/or survival of NSPCs and may contribute to the development of neural degenerative disease treatments based on the activation of endogenous NSPCs.
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Research Products
(13 results)
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[Journal Article] Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies2007
Author(s)
Ohta S, Ueda Y, Yaguchi M, Matsuzaki Y, Nakamura M, Toyama Y, Tanioka Y, Tamaoki N, Nomura T, Okano H, Kawakami Y, Toda M
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Journal Title
Immunology Nov.14(Epub ahead of print)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Identification of a neuron-specific human gene,KIAA1110,that is a guanine nucleotide exchange factor for ARF12007
Author(s)
Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M
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Journal Title
Biochem Biophys Res Commun 364・4
Pages: 737-742
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Preferential expression and frequent IgG responses of a tumor antigen, SOX5, in glioma patients2007
Author(s)
Ueda, R., Yoshida, K., Kawase, T., Kawakami, Y., Toda, M
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Journal Title
Int, J Cancer 120(8)
Pages: 1704-1711
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies2007
Author(s)
Ohta, S., Ueda, Y., Yaguchi, M., Matsuzaki, Y., Nakamura, M., Toyama, Y., Tanioka, Y., Tamaoki, N., Nomura, T., Okano, H., Kawakami, Y., Toda, M
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF12007
Author(s)
Hattori, Y., Ohta, S., Hamada, K., Yamada-Okabe, H., Kanemura, Y., Matsuzaki, Y., Okano, H., Kawakami, Y., Toda, M
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Journal Title
Biochem Biophys Res Commun 364(6)
Pages: 737-742
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Analysis of functional recovery by the implantation of Dendritic cells in non-human primate2007
Author(s)
Masahiro, Toda, Masae, Yaguchi, Masanao, Tabuse, Shigeki, Ohta, Kozo, Tsukada, Jun, Yamane, Masaya, Nakamura, Yumi, Matsuzaki, Yoshikuni, Tanioka, Takeshi, Kawase, Yoshiaki, Toyama, Hideyuki, Okano
Organizer
The Japanese society of Inflammation and regeneration, 28th meeting
Place of Presentation
Tokyo
Year and Date
2007-08-03
Description
「研究成果報告書概要(欧文)」より
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