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2007 Fiscal Year Final Research Report Summary

Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate in Purkinje cell synapses

Research Project

Project/Area Number 18500308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionGunma University

Principal Investigator

IINO Masae  Gunma University, Graduate School of Medicine, Assistant professor (20008329)

Project Period (FY) 2006 – 2007
KeywordsGlutamatergic synapse / GLT-1 / GLAST / Purkinje cell / Bergmann glia / Climbing fiber / EPSC / Transporter current
Research Abstract

In this study, I aimed to clarify roles of glial glutamate transporter GLT-1 in glutamatergic synapses. The results are summarized as follows: 1. We compared the decay kinetics of excitatory postsynaptic currents (EPSCs) in cerebellar Purkinje cells (PCs) evoked by parallel fiber (CF) stimulation among wild-type (WT), GLAST (-/-) and GLT-1 (-/-) mice. The decay time constant of CF-EPSCs (τ_w) in GLT-1 (-/-) mice was slower than that in WT mice. However, the degree of this prolongation of τ_w was less prominent compared to that in GLAST (-/-) mice. 2. The values of τ_w in GLT-1 (-/-) mice and GLAST (-/-) mice were comparable to those estimated in WT mice in the presence of a potent blocker of glial glutamate transporters (2S,3S)-3- [3- (4-methoxybenzoylamino) benzyloxy] aspartate (PMB-TBOA) at 10 nM and 100 nM, which reduced the amplitudes of glutamate transporter currents elicited by CF stimulation in BG to ?81% and ?28%, respectively. We conclude that GLT-1 plays a minor role compared to GLAST in clearing synaptically released glutamate at CF-PC synapses. 3. Pharmacological blockage of glial glutamate transporter functions caused a spillover of synaptically released glutamate from a single CF terminal over to neighboring PCs, indicating that glial glutamate transporters are indispensable to maintain one-to-one relationship at CF-PC synapses by preventing glutamate spillover. 4. In hippocampal CA1 neurons of newborn rats in which the glutamate synapses are not formed yet, pharmacological blockage of glial glutamate transporters caused a spontaneous depolarizing oscillations mediated by activation of NMDA receptors, suggesting that glial transporters play a pivotal role in clearing non-synaptically released glutamate to maintain its extracellular concentration at a low level.

  • Research Products

    (10 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (4 results)

  • [Journal Article] Contribution of glutamate transporter GLT-1, to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses2007

    • Author(s)
      Yusuke Takatsuru, Masae Iino, Kohichi Tanaka, and Seiji Ozawa.
    • Journal Title

      Neuroscience Letters 420

      Pages: 85-89

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Facilitated activation of metabotropic glutamate receptors in cerebell ar Purkinje cells in glutamate transporter EAAT4-deficient mice2007

    • Author(s)
      Osamu Nikkuni, Yukihiro Takayasu Masae Iino, Kohichi Tanaka, and Seiji Ozawa.
    • Journal Title

      Neuroscience Research 59

      Pages: 296-303

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses2007

    • Author(s)
      Yusuke, Takatsuru
    • Journal Title

      Nuroscience Letters 420

      Pages: 85-89

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Facilitated activation of metabotropic glutamate receptors in cerebellar Purkinje cells in glutamate transporter EAAT4-dificient mice2007

    • Author(s)
      Osamu, Nikkuni
    • Journal Title

      Neuroscience Research 59

      Pages: 296-303

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glial glutamate transporters maintain one-to-one relationship at the climbing fiber-Purkinje cell synapse by preventing glutamate spillover2007

    • Author(s)
      Yukihiro, Takayasu
    • Journal Title

      The Journal of Neuroscience 26

      Pages: 6563-6572

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glial glutamate transporters maintain one-to-one relationship at the climbing fiber-Purkinje cell synapse by preventing glutamate spillover2006

    • Author(s)
      Yukihiro Takayasu, Masae Iino, Keiko Shimamoto, Kohichi Tanaka, and Seiji Ozawa.
    • Journal Title

      The Journal of Neuroscience 26(24)

      Pages: 6563-6572

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Presentation] 小脳プルキンエ細胞シナプスにおけるグルタミン酸トランスポーターの機能的役割2008

    • Author(s)
      飯野昌枝、高安幸弘、小澤瀞司
    • Organizer
      第85回 日本生理学会大会
    • Place of Presentation
      京王プラザホテル東京
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional role of glutamate transporters in cerebellar Purkinje cell synapses2008

    • Author(s)
      Masae, Iimo
    • Organizer
      The 85th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Enhanced activation of metabotropic glutamate receptors in cerebellar Purkinje cells in EAAT4-decifient mice2006

    • Author(s)
      Yukihiro Takayasu, Osamu Nikkuni, Masae Iino, and Seiji Ozawa.
    • Organizer
      第36回 北米神経科学大会
    • Place of Presentation
      アトランタ
    • Year and Date
      2006-10-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Enhanced activation of metabotropic glutamate receptors in cerebellar Purkinje cells in EAAT4-decifient mice2006

    • Author(s)
      Yukihiro, Takayasu
    • Organizer
      Society for Neuroscience 36th Annual Meeting
    • Place of Presentation
      Atlanta, Georgia, USA
    • Year and Date
      2006-10-16
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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