2007 Fiscal Year Final Research Report Summary
Establishment of human hematopoietic system model mouse for understanding the regulatory mechanisms of human hematopoiesis.
| Project/Area Number |
18500332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
YAHATA Takashi Tokai University, School of Medicine, Assistant Professor (10398753)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Kiyoshi Tokai University, School of Medicine, Professor (70176014)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hematopoietic stern cells / niche / humanized mouse / mesehcnymal stem cells / intra-bone marrow transplantation / cord blood / cell cycle |
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| Research Abstract |
Hematopoiesis is maintained by specificinteractions between both hematopoietic and non-hematopoietic cells. While hematopoietic stem cells (HSCs) have been extensively studied both in vitro and in vivo, little is known about the in vivo characteristics of stem cells of the non-hematopoietic component, known as mesenchymal stem cells (MSCs). Here we have visualized and characterized human MSCs in vivo following intramedullary transplantation of enhanced green fluorescent protein-marked human MSCs (eGFP-MSCs) into the bone marrow (BM) of nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice. During 4 to 10 weeks after transplantation, eGFP-MSCs that engrafted in murine BM integrated into the hematopoietic microenvironment (HME) of the host mouse. They differentiated into pericytes, myofibroblasts, BM stromal cells, osteocytes in bone, bone-lining osteoblasts, and endothelial cells, which constituted the functional components of the BM HME. The presence of human MSCs in murine BM resulted in increase in functionally and phenotypically primitive human hematopoietic cells. Human MSC-derived cells that reconstituted the hematopoietic microenvironment appeared to contribute to the maintenance of human hematopoiesis by actively interacting with primitive human hematopoietic cells.
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