2007 Fiscal Year Final Research Report Summary
Establishment of preclinical study system for regenerative medicine using somatic nuclear transferred embryonic stem cells
| Project/Area Number |
18500336
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Central Institute for Experimental Animals |
|---|
Principal Investigator |
SASAKI Erika Central Institute for Experimental Animals, Marmoset Research Departmenet, Laboratory of Applied Developmental Biology, Lab Head (70390739)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SOTOMARU Yusuke Hiroshima University, Natural Science Center for Basic research and Deverlopment, Associate professor (90309352)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | Nuclear transfer / Developmental Biology / Primates |
|---|
| Research Abstract |
The nuclear transfer technique has been applied to various mammals to produce clone animals, but a standardized method is not applicable to all species. We examined here the optimum procedure for somatic cell cloning in common marmosets. In a preliminary experiment, we confirmed that parthenogenetic activation of in vitro matured oocytes was successfully induced by electrical stimulation (3 cycles of 150 V/mm, 50 μsec x 2, 20min intervals), and this condition was applied to the following experimental procedure. Using marmoset embryonic fibroblast cells as donor nuclei, nuclear transfer to recipient enucleated oocytes was performed 1h before, immediately after or 1h after activation treatment. After in vitro culture for 10 days, development to blastocyst stage was observed in only oocytes of which nuclei were transferred 1h before activation (4.3%), and in no other cases. We then examined the influence of the derivation of donor cells on development to blastocyst stage. Clone embryos were produced from donor myeloid cells from male and female adults and skin-derived fibroblasts from male adults. As a result, development to blastocyst stage was observed in 9.4% (myeloid from female), 14.5% (myeloid from male) and 9.3% (fibroblast from female) of clone embryos. Somatic cell cloned embryo with developmental ability to blastocyst stage is constructed when the donor cells have been transferred into the recipient, prior to activation.
|
-
[Journal Article] A novel embryonic stem cell line derived from the common marmoset monkey(Callithrix jacchus)exhibiting germ cell-like characteristics.2009
Author(s)
Muller,T., Fleischmann,G., Eildermann,K., Matz-Rensing,K., Horn,PA., Sasaki,E., Behr,R.
-
Journal Title
Human Reproduction 24
Pages: 1359-1372
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Growth characteristics of the non-human primate embryonic stem cell line cjes001 depending on feeder cell treatment.2009
Author(s)
Fleischmann,G., Muller,T., Behr,R., Blasczyk,R., Sasaki,E., Horn,P.A.
-
Journal Title
Cloning and Stem cells 11
Pages: 225-233
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Pre-implantation Development of Somatic Cell Cloned Embryos in the Common Marmoset(Callithrix jacchus).2009
Author(s)
Sotomaru,Y., Hirakawa,R., Shimada,A., Shiozawa,S., Sugawara,A., Oiwa,R., Nobukiyo,A., Okano,H., Tamaoki,N., Nomura,T., Hiyama,E., Sasaki,E.
-
Journal Title
Cloning and Stem cells 11
Pages: 575-583
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] A novel embryonic stem cell line derived from the common marmoset monkey(Callithrix jacchus)exhibiting germ cell-like characteristics2009
Author(s)
Muller, T., Fleischmann, G., Eildermann, K., Matz-Rensing, K., Horn, PA., Sasaki, E., Behr, R.
-
Journal Title
Hum.Repr. 24:13
Pages: 59-72
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Growth characteristics of the non-human primate embryonic stem cell line cjes001 depending on feeder cell treatment2009
Author(s)
Fleischmann, G., Muller, T., Behr, R., Blasczyk, R., Sasaki, E., Horn, P.A.
-
Journal Title
Cloning and Stem cells 11:2
Pages: 25-33
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Pre-implantation Development of Somatic Cell Cloned Embryos in the Common Marmoset(Callithrix jacchus)2009
Author(s)
Sotomaru, Y., Hirakawa, R., Shimada, A., Shiozawa, S., Sugawara, A., Oiwa, R., Nobukiyo, A., Okano, H., Tamaoki, N., Nomura, T., Hiyama, E., Sasaki, E.
-
Journal Title
Cloning and Stem cells 11:5
Pages: 75-83
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Journal Article] Tall/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset(Callithrix jacchus)ES cells2006
Author(s)
Kurita,R., Sasaki,E., Yokoo,T., Hiroyama,T., Takasugi,K., Izawa,K., Dong,Y., Hashiguchi,T., Soda,Y., Maeda,T., Suehiro,Y., Tanioka,Y., Nakazaki,Y., Tani,K.
-
Journal Title
Stem Cells 24
Pages: 2014-2022
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
-
[Journal Article] Tal1/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset(Callithrix jacchus)ES cells2006
Author(s)
Kurita, R., Sasaki, E., Yokoo, T., Hiroyama, T., Takasugi, K., Izawa, K., Dong, Y., Hashiguchi, T., Soda, Y., Maeda, T., Suehiro, Y., Tanioka, Y., Nakazaki, Y., Tani, K.
-
Journal Title
Stem Cells 24:20
Pages: 14-22
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Gene targeting and recombinase-mediated cassette exchange in ACTB locus of embryonic stem cells of the common marmoset2008
Author(s)
Shiozawa, S., Sasaki, E., Maeda, T., Suemizu, H., Ebukuro, M., Goto, K., Sotomaru, Y., Okano, H.
Organizer
The 31th Annual Meeting of Molecular Biology Society of Japan
Place of Presentation
Hyogo
Year and Date
2008-12-12
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] Establishment of non-invasive embryo collection in Common Marmoset2008
Author(s)
Sasaki, E., Oiwa, R., Hirakawa, R., Shimada, A., Kamioka, M., Hanazawa, K.
Organizer
The 55th Annual Meeting of the Japanese Association for Laboratory Animal Science
Place of Presentation
Miyagi
Year and Date
2008-05-16
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
