2007 Fiscal Year Final Research Report Summary
Development of a feed-back controlled culture system to maximize calcification of tissue-engineered hone promoted by strain-induced fluid flow
| Project/Area Number |
18500343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kanazawa University |
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Principal Investigator |
TANAKA Shigeo Kanazawa University, Institute of Nature and Environmental ental Technology, Associate Professor (20262602)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAKOSHI Ken-ichi Kanazawa University, Graduate School of Natural Sience and Technology, Professor (40014310)
KITAMURA Keiichiro Kanazawa University, Graduate School of Medical Science, Associate Professor (80283117)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Tissue engineering / Regenerative medicine / Bone / Osteoblast / Mechanical stimulation / Biomechanics / Cell / Calcification |
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| Research Abstract |
Stem cells have a potential to differentiate to specific organ cells and could be utilized to regenerate a lost organ with an appropriate scaffold in culture. This technology called tissue engineering or regenerative medicine is a promising strategy with a high biocompatibility avoiding the ethical problems in cadaveric donor transplantation and living donor transplantation Tissue-engineered bone composed of osteoblasts, a scaffold, and simulative hums was studied and developed in the past years, however it is still difficult to obtain sufficient calcification in vitro, comparing with natural bones. With this low mechano-compatibility, in particular a shortage of initial stiffness and toughness, tissue-engineered bone is not applicable to implantation to loaded bone sites. The purpose of this project is to development of a feed-back controlled culture system to maximize the calcification of tissue-engineered bone promoted by mechanical stimulation in fain of strain-induced fluid flow. This culture system is expected to provide better mechano-compatibility to an engineered bone before implantation. In this two-year project (2006-2007), two elemental technologies for the system were developed. In 2006, an optical device for non-destructive monitoring of in vitro calcification was developed using near-infrared light Using this device, the calcification of tissue-engineered bone composed of a type I collagen sponge and rat-primary cultured osteoblasts was monitored for 42 days in culture. In 3307, in the purpose of determination of initial condition of mechanical stimulation, a compact optical device was developed to observe concentration changes in intracellular calcium in osteoblasts populated in an engineered bone just after mechanical stimulation. These achievements should contribute to completion of the feed-hack controlled culture system for tissue-engineered bone.
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[Presentation] 再生骨の光センシング2008
Author(s)
田中茂雄、垣尾雅文、杉浦直樹、山越憲一
Organizer
第20回バイオエンジニアリング講演会
Place of Presentation
芝浦工業大学
Year and Date
20080125-26
Description
「研究成果報告書概要(和文)」より
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[Presentation] Development of optical monitoring System of osteoblastic calcification in vitro using near-infrared light2007
Author(s)
Masafumi, Kakio, Masamichi, Nogawa, Shinobu, Tanaka, Kenichi, Yamakoshi, Shigeo, Tanaka
Organizer
The 36th Graduate Student Conference, The Japan Society of Mechanical Engineering Hokuriku-Shinetsu Branch
Place of Presentation
Ishikawa
Year and Date
2007-03-07
Description
「研究成果報告書概要(欧文)」より
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