Comprehensive analysis of the time series of gene expressions caused by mechanical stretch of human pulmonary arterial endothelial cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 18500348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kitasato University
• Principal Investigator: NOSHIRO Makoto Kitasato University, 医療衛生学部, 教授 (80014231)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Hirosuke 北里大学, 医療衛生学部, 教授 (70153632) ; HARADA Yoshiteru 北里大学, 医療衛生学部, 教授 (20050677)
• Co-Investigator(Renkei-kenkyūsha): FUKUOKA Yutaka 東京医科歯科大学, 大学院・疾患生命科学研究部, 准教授 (30242217)
• Project Period (FY): 2006 – 2008
• Keywords: 人工呼吸関連肺損傷 / 伸展刺激 / 肺動脈内皮細胞 / 網羅的遺伝子発現解析 / 時系列

Research Abstract

機械的伸展によって, 肺動脈内皮細胞では加えた歪と等しい歪が, 肺胞上皮細胞では加えた歪より小さい歪が生じる. 繰り返し機械的伸展によって, 肺動脈内皮細胞から産生されるIL-6およびIL-8が伸展時間とともに増加する. 繰り返し機械的伸展によって生じる遺伝子発現変化は, 適応閾値によってより正確に検出できる. 繰り返し機械的伸展によって, 機械刺激受容体やカルシウム信号伝達などに関連する遺伝子やSELE, PPP1CBなどの発現が変化した

Research Products

• [Journal Article] The roll of nitric oxide in reducing deformability of Lewis lung tumor cell stimulated by inflammatory cytokines (2008)
  • Author(s): Kokubo K, Igawa S, Fukuda A, Shinbo T, Hirose M, Masuda N, Kobayashi H.
  • Journal Title: Nitric Oxide 19 Pages: 312-319
  • Peer Reviewed
• [Journal Article] A new implementation of the reverse engineering algorithm to elucidate gene functional dependencies (2007)
  • Author(s): H. Inaoka, Y. Fukuoka, A. Ueno, I.S. Kohane
  • Journal Title: IEEJ Transactions on Electronics, Information and Systems 127 Pages: 1712-1718
  • Peer Reviewed
• [Journal Article] Evidence of spatially-bound gene regulation in Mus Musculus : Decreased gene expression proximal to microRNA genomic location (2007)
  • Author(s): H Inaoka, Y Fukuoka, I S Kohane
  • Journal Title: Proc Natl Acad Sci USA 104, 12 Pages: 5020-5025
  • Peer Reviewed
• [Journal Article] Interaction between cyclooxygenase (COX)-1- and COX-2-products modulates COX-2 expression in the late phase of acute inflammation (2007)
  • Author(s): Nakano M, Denda N, Matsumoto M, Kawamura M, Kawakubo Y, Hatanaka K, Hiramoto Y, Sato Y, Noshiro M and Harada Y
  • Journal Title: Eur. J. Pharmacol 559 Pages: 210-218
  • Peer Reviewed
• [Journal Article] Macrophages that survive hyperoxia exposure have higher superoxide dismutase activities in their mitochondria
  • Author(s): K Kokubo, S Soeda, T Shinbo, M Hirose, N Fuku, Y Nishigaki, M Tanaka, H Kobayashi
  • Journal Title: Adv Exp Med Biol(in press)
  • Peer Reviewed
• [Presentation] Strain on the normal human pulmonary artery endothelial cells stretched mechanically (2008)
  • Author(s): K. Kobayashi, A. Kisi, M. Tanaka, H. Inaoka, S. Nebuya, Y. Fukuoka, H. Kobayashi, M. Noshiro
  • Organizer: Proc. Internat. Symp. Biol. Physiol. Enging.(311-312)
  • Place of Presentation: Harbin, China
  • Year and Date: 20080000
• [Presentation] シリコン膜の機械的伸展による細胞の伸展率 (2008)
  • Author(s): 小林こず恵、岸亜由美、田中真澄、稲岡秀検、根武谷吾、福岡豊、小林弘祐、野城真理
  • Organizer: 第47回日本生体医工学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Presentation] 適応閾値による遺伝子発現解析 (2008)
  • Author(s): 稲岡秀検, 福岡豊, 野城真理
  • Organizer: 第23回生体・生理工学シンポジウム論文集(157-159)
  • Place of Presentation: 名古屋
  • Year and Date: 20080000
• [Presentation] 肺動脈内皮細胞の機械的伸展による形状変化の測定 (2008)
  • Author(s): 小林こず恵, 岸亜由美, 田中真澄, 稲岡秀検, 根武谷吾, 小林弘祐, 野城真理
  • Organizer: 第23回生体・生理工学シンポジウム論文集(345-346)
  • Place of Presentation: 名古屋
  • Year and Date: 20080000
• [Presentation] Adaptive threshold to detect biologically meaningful changes in microarray data (2008)
  • Author(s): Y Fukuoka, H Inaoka, M Noshiro
  • Organizer: Proc. 30th Ann Int IEEE EMBS Conf(5708-5711)
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20080000
• [Presentation] 適応閾値を用いた発現解析 (2007)
  • Author(s): 稲岡秀検, 福岡豊, 野城真理
  • Organizer: システム・情報部門学術講演会2007講演論文集(41-42)
  • Place of Presentation: 東京
  • Year and Date: 20070000
• [Presentation] 機械伸展による細胞の伸展率 (2007)
  • Author(s): 小林こず恵, 岸亜由美, 田中真澄, 稲岡秀検, 根武谷吾, 小林弘祐, 野城真理
  • Organizer: システム情報部門学術講演会2007 講演論文集(177-178)
  • Place of Presentation: 東京
  • Year and Date: 20070000
• [Presentation] Adaptive threshold for detecting significant fold changes in microarray data (2006)
  • Author(s): H Inaoka, Y Fukuoka
  • Organizer: The 17th International Conference on Genome Informatics
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20061200
• [Presentation] 遺伝子発現に基づく細胞機能評価の試み (2006)
  • Author(s): 福岡豊, 稲岡秀検, 野城真理
  • Organizer: 第21回生体・生理工学シンポジウム (鹿児島)
  • Year and Date: 20061100